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Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

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Abstract

Background

The river prawn, Macrobrachium americanum (M. americanum), is one of the largest prawns of the genus in Latin America and is an amphidromous species distributed along the Pacific coast of America. This prawn has commercial value due to its size and taste, making it a good option for aquaculture production. Its culture has been attempted in ponds and concrete tanks, but no successful technique can still support commercial production. Understanding the mechanisms that regulate reproduction at the molecular level is very important. This knowledge can provide tools for manipulating transcripts, which could increase the number or size of animals in the culture. Our understanding of the mechanism that regulates the reproduction of M. americanum at the molecular level is limited.

Aim

Perform and analyze the transcriptome assembly of the testes, vas deferens, and terminal ampulla of M. americanum. to provide new molecular information about its reproduction.

Methods and Results

The cDNA library was constructed and sequenced for each tissue to identify novel transcripts. A combined transcriptome with the three tissues was assembled using Trinity software. Unigenes were annotated using BLASTx and BLAST2GO. The transcriptome assembly generated 1,059,447 unigenes, of which 7222 genes had significant hits (e-value < 1 × 10–5) when compared against the Swiss-Prot database. Around 75 genes were related to sex determination, testis development, spermatogenesis, spermiogenesis, fertilization, maturation of testicular cells, neuropeptides, hormones, hormone receptors, and/or embryogenesis.

Conclusions

These results provide new molecular information about M. americanum reproduction, representing a reference point for further genetic studies of this species.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

We thank Consejo Nacional de Ciencia y Tecnología (CONACYT) for financially supporting Miriam Victoria Martín-Manzo, and Maritza Lourdes Soberanes-Yepiz postdoctoral position at the Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR). Manuel Vargas and Omar Almaraz (U de Guadalajara) provided technical support in river prawn recollection.

Funding

This study was funded by Consejo Nacional de Ciencia y Tecnología (CONACYT) (Grant FC2016/2930).

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Authors and Affiliations

  1. Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Playa Palo de Santa Rita Sur, Av. Instituto Politécnico Nacional 195, 23096, La Paz, BCS, Mexico

    Miriam Victoria Martín-Manzo, Maritza Lourdes Soberanes-Yepiz & Edilmar Cortés-Jacinto

  2. Centro de Investigaciones Biológicas del Noroeste Tepic, Investigadoras E Investigadores Por México-CONACYT. Unidad Nayarit, Nayarit, Mexico

    Rosa María Morelos-Castro

  3. Applied Genomics Lab, Av. Gral. Félix Ortega Aguilar, 23000, La Paz, Baja California Sur, Mexico

    Adrian Munguia-Vega

  4. Conservation Genetics Laboratory, The University of Arizona, Tucson, AZ, 85721, USA

    Adrian Munguia-Vega

Authors
  1. Miriam Victoria Martín-Manzo
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  2. Rosa María Morelos-Castro
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  3. Adrian Munguia-Vega
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  4. Maritza Lourdes Soberanes-Yepiz
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  5. Edilmar Cortés-Jacinto
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Contributions

Edilmar Cortés-Jacinto contributed to the study conception and funding acquisition. Rosa María Morelos-Castro and Adrian Munguia-Vega designed, supervised, and advised the methodology. Material preparation and data collection were performed by Miriam Victoria Martín-Manzo and Maritza Lourdes Soberanes-Yepiz. Formal Analysis and investigation were performed, the main manuscript was written by Miriam Victoria Martín-Manzo. All authors reviewed and approved the final manuscript.

Corresponding author

Correspondence to Edilmar Cortés-Jacinto.

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The authors declare no competing interests.

Ethical approval

The study was carried out strictly following the Standard Operating Procedures (SOP) for the Use of Experimental Animals of the Centro de Investigaciones del Noroeste, S.C. (CIBNOR).

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Martín-Manzo, M.V., Morelos-Castro, R.M., Munguia-Vega, A. et al. Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum. Mol Biol Rep 51, 259 (2024). https://doi.org/10.1007/s11033-023-09125-6

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