Abstract
ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene—ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis and contains a potential fusion domain. A full-length cDNA ofADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalianADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268—Asp374) as immunogen. The result of immunohistochemical test on human testis showed thatADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of testis-associated cells.
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Wang, F., Xu, R., Zhu, P. et al. Preliminarily functional analysis of a cloned novel human geneADAM29 . Sci. China Ser. C.-Life Sci. 44, 392–399 (2001). https://doi.org/10.1007/BF02879606
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DOI: https://doi.org/10.1007/BF02879606