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The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats

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Abstract

Background

Alpha-pinene (α-pinene) is a monoterpene with gastroprotective activity. We evaluated the gastroprotective effect of α-pinene in the gastric damage model with ethanol.

Methods

We evaluated the macroscopic evaluation of the stomach cavity, alteration in pH, mRNA expression of nuclear factor erythroid 2- related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), total antioxidant capacity (TAC) levels, and histopathological changes.

Results

Pretreatment with α-pinene (10, 50 and 100 mg/kg i.p.) before oral administration of ethanol reduced gastric mucosal damage by increasing the percentage of ulcer inhibition. Alpha-pinene also increased gastric pH similar to omeprazole. In addition, the histopathological examination showed that in the groups pretreated with α-pinene 50 and 100 mg/kg, and omeprazole20 mg/kg, the lesions were less than the control group. Moreover, α- pinene 10, 50, 100, and omeprazole 20 mg/kg upregulated the NRF2 and HO1.

Conclusions

Our results show that pretreatment with α-pinene is effective in reducing ethanol-induced gastric damage through regulation of Nrf2/HO-1.

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Data availability

All data are available upon request.

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Funding

Funding provided by the Faculty of Veterinarey Medicine University of Shahid Chamran Ahvaz Iran.

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Authors and Affiliations

  1. Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Kaveh Rahimi, Negin Shirvani, Parham Sanaie, Arian Javadi & Mahsa Khademi

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  1. Kaveh Rahimi
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  2. Negin Shirvani
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  3. Parham Sanaie
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  4. Arian Javadi
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  5. Mahsa Khademi
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Contributions

Conceptualization, Methodology, Formal analysis, Investigation, Data Collection, Writing, Review and Editing, KR, NS, PS, AJ, MKh. Project Administration, KR; Funding Acquisition, KR. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Kaveh Rahimi.

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Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This study approved by the Ethics Committee of the Faculty of Veterinarey Medicine University of Shahid Chamran Ahvaz Iran (Code of ethics: EE/1401.2.24.226200/scu.ac.ir).

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Rahimi, K., Shirvani, N., Sanaie, P. et al. The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats. Mol Biol Rep 50, 8615–8622 (2023). https://doi.org/10.1007/s11033-023-08765-y

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  • DOI: https://doi.org/10.1007/s11033-023-08765-y

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