Abstract
The occurrence of PFD is closely related with elasticity, toughness, and functional changes of the connective tissue of the pelvic support tissue. This study aims to evaluate the effect of mechanical stretch on the differentiation of BMSCs with a co-culture with pelvic ligament fibroblasts. BMSCs was isolated and identified from 7 day SPF SD rats. Rat pelvic ligament fibroblasts were obtained from rat pelvic ligament. The fourth passage of fibroblasts was subjected to 10% deformation with 1 Hz, 12 h periodic one-way mechanical stretch stimulation, and the cells were then co-cultured with BMSCs. The longer co-culture and co-culture with mechanical stretch (i.e. 6 and 12 days) induced more expression of elastin, LOX, and Fibulin-5, compared to the groups without stimulation. Compared to co-culture group each, Co-culture with mechanical stretch stimulation group induced significant expression of elastin, LOX, and Fibulin-5, both in 3, 6 and 12 days co-culture groups (P < 0.05). However, there were no significant differences among 3, 6, and 12 days control groups. These results suggest that in an indirect co-culture system, pelvic ligament fibroblasts with mechanical stretch stimulation can promote BMSCs differentiation, reflecting in the increased expression of elastin, LOX, and Fibulin-5.
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Abbreviations
- BMSCs:
-
Bone mesenchymal stem cells
- LOX:
-
Lysyl oxidase
- PFD:
-
Pelvic floor dysfunction
- POP:
-
Pelvic organ prolapse
- SUI:
-
Stress urinary incontinence
- ECM:
-
Extracellular matrix components
References
Chiquet M (1999) Regulation of extracellular matrix gene expression by mechanical stress. Matrix Biol 18:417–426
Drewes PG, Yanagisawa H, Starcher B, Hornstra I, Csiszar K, Marinis SI, Keller P, Word RA (2007) Pelvic organ prolapse in fibulin-5 knockout mice: pregnancy-induced changes in elastic fiber homeostasis in mouse vagina. Am J Pathol 170:578–589
Fermor B, Gundle R, Evans M, Emerton M, Pocock A, Murray D (1998) Primary human osteoblast proliferation and prostaglandin E2 release in response to mechanical strain in vitro. Bone 22:637–643
Guo Z, Yang J, Liu X, Li X, Hou C, Tang PH, Mao N (2001) Biological features of mesenchymal stem cells from human bone marrow. Chin Med J (Engl) 114:950–953
Hankemeier S, Hurschler C, Zeichen J, van Griensven M, Miller B, Meller R, Ezechieli M, Krettek C, Jagodzinski M (2009) Bone marrow stromal cells in a liquid fibrin matrix improve the healing process of patellar tendon window defects. Tissue Eng Part A 15:1019–1030
Horikawa A, Okada K, Sato K, Sato M (2000) Morphological changes in osteoblastic cells (MC3T3–E1) due to fluid shear stress: cellular damage by prolonged application of fluid shear stress. Tohoku J Exp Med 191:127–137
Juncosa-Melvin N, Shearn JT, Boivin GP, Gooch C, Galloway MT, West JR, Nirmalanandhan VS, Bradica G, Butler DL (2006) Effects of mechanical stimulation on the biomechanics and histology of stem cell-collagen sponge constructs for rabbit patellar tendon repair. Tissue Eng 12:2291–2300
Katsuta Y, Ogura Y, Iriyama S, Goetinck PF, Klement JF, Uitto J, Amano S (2008) Fibulin-5 accelerates elastic fibre assembly in human skin fibroblasts. Exp Dermatol 17:837–842
Kerkhof MH, Hendriks L, Brolmann HA (2009) Changes in connective tissue in patients with pelvic organ prolapse–a review of the current literature. Int Urogynecol J Pelvic Floor Dysfunct 20:461–744
Kjaer M (2004) Role of extracellular matrix in adaptation of tendon and skeletal muscle to mechanical loading. Physiol Rev 84:649–698
Kobak W, Lu J, Hardart A, Zhang C, Stanczyk FZ, Felix JC (2005) Expression of lysyl oxidase and transforming growth factor beta2 in women with severe pelvic organ prolapse. J Reprod Med 50:827–831
Lee IC, Wang JH, Lee YT, Young TH (2007) The differentiation of mesenchymal stem cells by mechanical stress or/and co-culture system. Biochem Biophys Res Commun 352:147–152
Li H, Yu B, Zhang Y, Pan Z, Xu W, Li H (2006) Jagged1 protein enhances the differentiation of mesenchymal stem cells into cardiomyocytes. Biochem Biophys Res Commun 341:320–325
Liu G, Daneshgari F, Li M, Lin D, Lee U, Li T, Damaser MS (2007) Bladder and urethral function in pelvic organ prolapsed lysyl oxidase like-1 knockout mice. BJU Int 100:414–418
Miller MD, Nichols T, Butler CA (1999) Patella fracture and proximal patellar tendon rupture following arthroscopic anterior cruciate ligament reconstruction. Arthroscopy 15:640–643
Omoto M, Miyashita H, Shimmura S, Higa K, Kawakita T, Yoshida S, McGrogan M, Shimazaki J, Tsubota K (2009) The use of human mesenchymal stem cell-derived feeder cells for the cultivation of transplantable epithelial sheets. Invest Ophthalmol Vis Sci 50:2109–2115
Rahn DD, Acevedo JF, Word RA (2008) Effect of vaginal distention on elastic fiber synthesis and matrix degradation in the vaginal wall: potential role in the pathogenesis of pelvic organ prolapse. Am J Physiol Regul Integr Comp Physiol 295:R1351–R1358
Rodriguez JP, Astudillo P, Rios S, Pino AM (2008) Involvement of adipogenic potential of human bone marrow mesenchymal stem cells (MSCs) in osteoporosis. Curr Stem Cell Res Ther 3:208–218
Sarasa-Renedo A, Chiquet M (2005) Mechanical signals regulating extracellular matrix gene expression in fibroblasts. Scand J Med Sci Sports 15:223–230
Smith AN, Willis E, Chan VT, Muffley LA, Isik FF, Gibran NS, Hocking AM (2010) Mesenchymal stem cells induce dermal fibroblast responses to injury. Exp Cell Res 316(1):48–54
Suzme R, Yalcin O, Gurdol F, Gungor F, Bilir A (2007) Connective tissue alterations in women with pelvic organ prolapse and urinary incontinence. Acta Obstet Gynecol Scand 86:882–888
Tsuruga E, Yajima T, Irie K (2004) Induction of fibulin-5 gene is regulated by tropoelastin gene, and correlated with tropoelastin accumulation in vitro. Int J Biochem Cell Biol 36:395–400
Wynn RF, Hart CA, Corradi-Perini C, O’Neill L, Evans CA, Wraith JE, Fairbairn LJ, Bellantuono I (2004) A small proportion of mesenchymal stem cells strongly expresses functionally active CXCR4 receptor capable of promoting migration to bone marrow. Blood 104:2643–2645
Xu WX, Kim SJ, So I, Kim KW (1997) Role of actin microfilament in osmotic stretch-induced increase of voltage-operated calcium channel current in guinea-pig gastric myocytes. Pflugers Arch 434:502–504
Xu YX, Chen L, Hou WK, Lin P, Sun L, Sun Y, Dong QY, Liu JB, Fu YL (2009) Mesenchymal stem cells treated with rat pancreatic extract secrete cytokines that improve the glycometabolism of diabetic rats. Transplant Proc 41:1878–1884
Yamamoto K, Yamamoto M, Akazawa K, Tajima S, Wakimoto H, Aoyagi M (1997) Decrease in elastin gene expression and protein synthesis in fibroblasts derived from cardinal ligaments of patients with prolapsus uteri. Cell Biol Int 21:605–611
Young RG, Butler DL, Weber W, Caplan AI, Gordon SL, Fink DJ (1998) Use of mesenchymal stem cells in a collagen matrix for Achilles tendon repair. J Orthop Res 16:406–413
Acknowledgments
We thank Dr. Zhao Ya and Jiang He (Department of Pathogen Biology, School of Fourth Military Medical University, China) for their assistance with preparation of the experiment.
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Bing, Z., Linlin, L., Jianguo, Y. et al. Effect of mechanical stretch on the expressions of elastin, LOX and Fibulin-5 in rat BMSCs with ligament fibroblasts co-culture. Mol Biol Rep 39, 6077–6085 (2012). https://doi.org/10.1007/s11033-011-1422-x
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DOI: https://doi.org/10.1007/s11033-011-1422-x