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The osteogenic differentiation of dog bone marrow mesenchymal stem cells in a thermo-sensitive injectable chitosan/collagen/β-glycerophosphate hydrogel: in vitro and in vivo

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Abstract

Type I collagen was added to the composite chitosan solution in a ratio of 1:2 to build a physical cross-linked self-forming chitosan/collagen/β-GP hydrogel. Osteogenic properties of this novel injectable hydrogel were evaluated. Gelation time was about 8 min which offered enough time for handling a mixture containing cells and the subsequent injection. Scanning electronic microscopy (SEM) observations indicated good spreading of bone marrow mesenchymal stem cells (BMSCs) in this hydrogel scaffold. Mineral nodules were found in the dog-BMSCs inoculated hydrogel by SEM after 28 days. After subcutaneous injection into nude mouse dorsum for 4 weeks, partial bone formation was observed in the chitosan/collagen/β-GP hydrogel loaded with pre-osteodifferentiated dog-BMSCs, which indicated that chitosan/collagen/β-GP hydrogel composite could induce osteodifferentiation in BMSCs without exposure to a continual supply of external osteogenic factors. In conclusion, the novel chitosan/collagen/β-GP hydrogel composite should prove useful as a bone regeneration scaffold.

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Acknowledgment

This work was partly supported by the National Science Foundation of China (NSFC: 50901088).

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Authors and Affiliations

  1. Institute of Stomatology, Chinese PLA General Hospital, Fuxing Road 28, Haidian District, Beijing, 100853, China

    Bin Sun, Fang Su, Yi Wang, Jiaqiang Liu, Dongshen Wang & Hongchen Liu

  2. Department of Oral Implant, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, 710032, China

    Wei Ma

Authors
  1. Bin Sun
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  2. Wei Ma
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  3. Fang Su
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  4. Yi Wang
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  5. Jiaqiang Liu
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  6. Dongshen Wang
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  7. Hongchen Liu
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Corresponding author

Correspondence to Hongchen Liu.

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Bin Sun and Wei Ma have contributed equally to this work.

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Sun, B., Ma, W., Su, F. et al. The osteogenic differentiation of dog bone marrow mesenchymal stem cells in a thermo-sensitive injectable chitosan/collagen/β-glycerophosphate hydrogel: in vitro and in vivo. J Mater Sci: Mater Med 22, 2111–2118 (2011). https://doi.org/10.1007/s10856-011-4386-4

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  • DOI: https://doi.org/10.1007/s10856-011-4386-4

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