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Semipermeable barrier-assisted electrophoretic deposition of robust collagen membranes

  • Materials for life sciences
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Abstract

Collagen membranes with high robustness and a defined stable biodegradation are of a great interest for dental surgery. Electrophoretic deposition (EPD) is a perspective candidate technology for their production, and in this article, the authors demonstrated the capabilities of its modification, a semipermeable barrier-assisted EPD (SBA-EPD). The SBA-EPD process was carried out at 60 V for 1,2, or 3 cycles 20 min each, after which the obtained membranes were crosslinked with 0.625% genipin. SBA-EPD allowed for the fabrication of membranes with high collagen packing density (0.0012 g/mm3) and high robustness, which depended on the number of SBA-EPD cycles. The highest Young’s modulus, tensile strength, and strain at failure values of non-crosslinked samples corresponded to 3-cycle deposition (15.11 ± 1.78 MPa, 4.2 ± 1.6 MPa, 60 ± 12%, respectively). However, crosslinking diminished the statistical difference of these parameters among membranes of different deposition cycles (reaching for 3-cycle deposition 64 ± 5 MPa, 9.5 ± 1.1 MPa, 23.7 ± 2.0%, respectively). On day 21 after subcutaneous implantation, the non-crosslinked membranes showed a significant degree of resorption higher than that of crosslinked ones by 3.5 times in absolute values. As well, crosslinked membranes elicited a greater peri-implant pro-fibrotic and giant cell response. Overall, the mechanical characteristics of SBA-EPD membranes were mostly superior to commercially available products used for dental applications, while their biodegradation timeframes fitted the optimal window. The authors hope that their research will attract the attention to SBA-EPD as an emerging and perspective technology for the production of robust and defect-free collagen membranes for dental surgery.

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Acknowledgements

The authors would like to thank Anastasia Kurenkova for the help with the proper statistical analysis of the registered data and Viktoria Chernikovich for drawing graphical abstract. The study was carried out using the unique scientific facility Transgenebank. This study was financially supported by the RSF Grant No. 23-15-00481 (membrane production, physicochemical characterization, animal experiments). Research at Sechenov University in the part of the cell experiments and mechanical characterization was funded by the Ministry of Science and Higher Education of the Russian Federation under the grant agreement No. 075-15-2021-596.

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Authors and Affiliations

  1. World-Class Research Center “Digital Biodesign and Personalized Healthcare”, Sechenov University, 8-2 Trubetskaya St., Moscow, Russia, 119991

    Artem Antoshin, Polina Bikmulina, Alexey Fayzullin, Anastasia Shpichka & Peter Timashev

  2. Skolkovo Institute of Science and Technology, 1 Bolshoy Boulevard, 30, Moscow, Russia, 121205

    Oleg Dubinin & Stanislav Evlashin

  3. World-Class Research Center, Advanced Digital Technologies, Saint Petersburg State Marine Technical University, 3 Lotsmanskaya St., Saint-Peterburg, Russia, 190121

    Oleg Dubinin

  4. Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Lei Miao, Xue Qu & Changsheng Liu

  5. Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., Moscow, Russia, 119991

    Elena Istranova, Azat Magdanov, Nastasia Kosheleva, Svetlana Kotova, Yuri Efremov, Anastasia Shpichka & Peter Timashev

  6. Federal State Budgetary Institution of Science “M.M. Krasnov Research Institute of Eye Diseases”, 11A Rossolimo St., Moscow, Russia, 119021

    Marina Kravchik

  7. FSBSI Institute of General Pathology and Pathophysiology, 8 Baltiyskaya, Moscow, Russia, 125315

    Nastasia Kosheleva

  8. N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 4 Kosygin St., Moscow, Russia, 119991

    Anna Solovieva & Svetlana Kotova

  9. Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow, Russia, 119344

    Elena Sadchikova

  10. Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya Street, Moscow, Russia, 119991

    Denis Butnaru

  11. Laboratory of Clinical Smart Nanotechnologies, Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., Moscow, Russia, 119991

    Polina Bikmulina, Nastasia Kosheleva, Anastasia Shpichka & Peter Timashev

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  1. Artem Antoshin
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  2. Oleg Dubinin
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Contributions

AA, OD, LM, CL, and PT outlined the manuscript. EI and AM prepared and characterized the properties of the collagen suspension. OD and LM performed and described the process of SBA-EPD. YE and ASo measured the membranes’ physicochemical parameters and revealed their crosslinking-related changes. PB, NK, ASh performed the cell experiments and interpreted their results. MK carried out the SEM analysis of the membranes and cells. AF, DB carried out the animal experiments and the subsequent histomorphometric analysis. AA, OD, LM, PB, AF, SK, ASo, AM, MK, ES, YE, XQ, SE, CL, and PT contributed to the discussion. AA and OD drafted the manuscript with editing and revision support from ASh, XQ, LM, ES, PB, AF, EI, NK, SK, DB, SE, CL, and PT. AA and PT coordinated the manuscript preparation. All the authors have read and approved the final manuscript.

Corresponding author

Correspondence to Peter Timashev.

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The authors declare no conflict of interest.

Ethical approval

The study was approved by the Local Ethical Committee at the Sechenov University (Moscow, Russia). The experiments on animals were conducted in the vivarium at the Sechenov University in accordance with the European Convention (Strasbourg, 1986) and the World Medical Association Declaration of Helsinki on the human treatment of animals (2000).

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Antoshin, A., Dubinin, O., Miao, L. et al. Semipermeable barrier-assisted electrophoretic deposition of robust collagen membranes. J Mater Sci 58, 9675–9697 (2023). https://doi.org/10.1007/s10853-023-08641-x

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