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The genotypic and phenotypic interaction of wheat and rye storage proteins in primary triticale

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Abstract

The creation of new triticale germplasm from its wheat and rye progenitors is an important source of potentially beneficial alleles. This research focused on the inheritance of glutenin and secalin alleles in triticale and their interaction in the hybrid offspring in terms of both subunit expression and gluten strength. Multiple crosses between five durum lines and two inbred rye lines were performed to create a set of 23 fertile amphidiploid lines. The SDS-PAGE banding patterns of the denatured HMW glutenins, LMW glutenins, HMW secalins and 75k γ-secalins (termed secaloglutenin) were compared to their exact parental plants, and their SDS-sedimentation was evaluated. Secaloglutenin subunits were simply inherited and expressed in all primary triticales. The few observed variations in banding patterns were accounted for within measured heterogeneity of the exact rye parental plants, for which the secalin alleles were thoroughly described. However, the possibility remains that mixed protein oligomers were formed between polymeric and monomeric storage proteins at the ultrastructure level. Furthermore, significant differences in the gluten strength of primaries derived from different durum parents were observed. This research suggests triticale of high gluten strength can be obtained by selection of parents with favourable glutenin alleles.

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References

  • Altpeter F, Popelka JC, Wieser H (2004) Stable expression of 1Dx5 and 1Dy10 high-molecular-weight glutenin subunit genes in transgenic rye drastically increases the polymeric glutelin fraction in rye flour. Plant Mol Biol 54:783–792

    Article  PubMed  CAS  Google Scholar 

  • Amiour N, Bouguennec A, Marcoz C, Sourdille P, Bourgoin M, Khelifi D, Branlard G (2002) Diversity of seven glutenin and secalin loci within triticale cultivars grown in Europe. Euphytica 123:295–305

    Article  CAS  Google Scholar 

  • Balatero CH, Darvey NL (1993) Influence of selected wheat and rye genotypes on the direct synthesis of hexaploid triticale. Euphytica 66:179–185

    Article  Google Scholar 

  • Bernard M, Bernard S, Joudrier P, Autran JC (1990) Biochemical characterization of lines descended from 8x triticale × 4x triticale cross. Theor Appl Genet 79:646–653

    Article  CAS  Google Scholar 

  • Branlard G, Dardevet M (1985) Diversity of grain protein and bread wheat quality. II. Correlation between high molecular weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354

    Article  CAS  Google Scholar 

  • Chen CH, Bushuk W (1970) Nature of proteins in triticale and its parental species. I. Solubility characteristics and amino acid composition of endosperm proteins. Can J Plant Sci 50:9–14

    Article  CAS  Google Scholar 

  • Ciaffi M, Lan Q, Lafiandra D, Tomassini C, Porfiri O, Giorgi B (1991) Relationship between electrophoretic patterns of storage proteins and breadmaking quality in triticale. In: Proceedings of the 2nd international triticale symposium, Passo Fundo, Rio Grande do Sul, Brazil, 1–5 Oct, 1990

  • Cooper KV, Driscoll CJ (1985) The production of primary triticales and the concept of adaptation to marginal conditions. In: Bernard B, Bernard S (eds) Proceedings of the 3rd EUCARPIA meeting on genetics and breeding of triticale, Clermont-Ferrand, France, 2–5 July 1984. EUCARPIA, France

  • De Bustos A, Jouve N (2003) Characterisation and analysis of new HMW-glutenin alleles encoded by the Glu-R1 locus of Secale cereale. Theor Appl Genet 107:74–83

    PubMed  Google Scholar 

  • Dick JW, Quick JS (1983) A modified screening test for rapid estimation of gluten strength in early-generation durum wheat breeding lines. Cereal Chem 60:315–318

    Google Scholar 

  • Dreisigacker S, Kishii M, Lage J, Warburton M (2008) Use of synthetic hexaploid wheat to increase diversity for CIMMYT bread wheat improvement. Aust J Agric Res 59:413–420

    Article  Google Scholar 

  • Field JM, Shewry PR (1987) Prolamin subunit interactions in hexaploid and octaploid triticales and their wheat and rye parents. J Cereal Sci 6:199–210

    Article  CAS  Google Scholar 

  • Gellrich C, Schieberle P, Wieser H (2003) Biochemical characterization and quantification of the storage protein (secalin) types in rye flour. Cereal Chem 80:102–109

    Article  CAS  Google Scholar 

  • McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris CF, Somers DJ, Appels R, Devos KM Catalogue of gene symbols for wheat. In: Appels R, Eastwood R, Lagudah E et al. (eds) 11th international wheat genetics symposium, Brisbane, QLD, Australia, 24–29 August 2008. Sydney University Press, Sydney

  • Mergoum M, Gomez Macpherson H (2004) Triticale improvement and production. Food and Agriculture Organization of the United Nations, Rome

  • Oettler G (1983) Crossability and embryo development in wheat-rye hybrids. Euphytica 32:593–600

    Article  Google Scholar 

  • Oettler G (1984) Parental effects on crossability, embryo differentiation and plantlet recovery in wheat × rye hybrids. Euphytica 33:233–239

    Article  Google Scholar 

  • Oettler G (1985) The effect of wheat cytoplasm on the synthesis of wheat X rye hybrids. Theor Appl Genet 71:467–471

    Article  Google Scholar 

  • Orth RA, Dronzek BL, Bushuk W (1974) Studies of glutenin. VII. Inheritance of its physicochemical factors in triticale. Cereal Chem 51:281–288

    CAS  Google Scholar 

  • Peña RJ, Pfeiffer WH, Amaya A, Zarco-Hernandez J (1991) High molecular weight glutenin subunit composition in relation to the bread making quality of spring triticale. In: Martin EJ, Wrigley W (eds) Cereals international, Brisbane, Australia, Sept 9–13, 1991. Royal Australian Chemical Institute, North Melbourne

  • Rozynek B, Gunther T, Hesemann CU (1998) Gel electrophoretic investigations of prolamins in eu- and alloplasmatic octoploid primary triticale forms. Theor Appl Genet 96:46–51

    Article  CAS  Google Scholar 

  • Salmanowicz BP, Dylewicz M (2007) Identification and characterization of high-molecular-weight glutenin genes in Polish triticale cultivars by PCR-based DNA markers. J Appl Genet 48:347–357

    Article  PubMed  Google Scholar 

  • Salmanowicz BP, Nowak J (2009) Diversity of monomeric prolamins in triticale cultivars determined by capillary zone electrophoresis. J Agric Food Chem 57:2119–2125

    Article  PubMed  CAS  Google Scholar 

  • Shewry PR, Halford NG (2001) Cereal seed storage proteins: structures, properties and role in grain utilization. J Exp Bot 53:947–958

    Article  Google Scholar 

  • Sirkka A, Immonen T, Varughese G, Pfeiffer WH, Mujeebkazi A (1993) Crossability of tetraploid and hexaploid wheats with ryes for primary triticale production. Euphytica 65:203–210

    Article  Google Scholar 

  • Tikhenko ND, Tsvetkova NV, Voylokov AV (2005) Genetic control of embryo lethality in crosses between common wheat and rye. Russ J Genet 41:877–884

    Article  CAS  Google Scholar 

  • Tikhenko N, Rutten T, Voylokov A, Houben A (2008) Analysis of hybrid lethality in F1 wheat–rye hybrid embryos. Euphytica 159:367–375

    Article  Google Scholar 

  • Tohver M, Kann A, Taht R, Mihhalevski A, Hakman J (2005) Quality of triticale cultivars suitable for growing and bread-making in northern conditions. Food Chem 89:125–132

    Article  CAS  Google Scholar 

  • Varughese G, Pfeiffer WH, Peña RJ (1996) Triticale: a successful alternative crop (Part 2). Cereal Food World 41:635–645

    Google Scholar 

  • Virdi BV, Larter EN (1984) The influence of whea–rye parental species in the protein characteristics of primary triticale. Can J Genet Cytol 26:258–263

    CAS  Google Scholar 

  • Zhang J, Tang Z, Fu S, Yan B, Ren Z (2008) The effect of different rye chromosomes on the expression of storage protein in common wheat. J Triticeae Crops 28:232–237

    CAS  Google Scholar 

Download references

Acknowledgments

This work is funded by the Grains Research and Development Corporation. Many thanks to Mike Sissons (DPI Tamworth) and Britt Kalmeier (AGT) for providing seed, and to Zhao Xiochun, Jeremy Roake, Hassan and Nizam Ahmed (University of Sydney), and Joop van Leur (DPI Tamworth) for their invaluable technical assistance.

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Authors and Affiliations

  1. Plant Breeding Institute, University of Sydney, 12656 Newell Highway, Narrabri, NSW, 2390, Australia

    Angela L. Dennett & Richard M. Trethowan

  2. KV Cooper and MG Elleway, PO Box 689, Stirling, SA, 5152, Australia

    Katharine V. Cooper

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  1. Angela L. Dennett
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  2. Katharine V. Cooper
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  3. Richard M. Trethowan
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Correspondence to Angela L. Dennett.

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Dennett, A.L., Cooper, K.V. & Trethowan, R.M. The genotypic and phenotypic interaction of wheat and rye storage proteins in primary triticale. Euphytica 194, 235–242 (2013). https://doi.org/10.1007/s10681-013-0950-y

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  • DOI: https://doi.org/10.1007/s10681-013-0950-y

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