Skip to main content

Advertisement

SpringerLink
Log in

Mapping of QTLs associated with cadmium tolerance and accumulation during seedling stage in rice (Oryza sativa L.)

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Cadmium (Cd) is a non-essential element and toxic to plants. To investigate the genetics of Cd tolerance and accumulation in rice, quantitative trait loci (QTL) associated with Cd tolerance and accumulation at the seedling stage were mapped using a doubled haploid (DH) population derived from a cross between a japonica JX17 and an indica ZYQ8. A total of 22 QTLs were found to be associated with shoot height (SH), root length (RL), shoot dry weight (SDW), root dry weight (RDW), total dry weight (TDW) and chlorophyll content (CC), and 10 and 12 QTLs were identified under the control and Cd stress conditions, respectively. For Cd tolerant coefficient (CTC), 6 QTLs were detected on chromosomes 1, 3, 5, 8 and 10. Under Cd stress, 3 QTLs controlling root and shoot Cd concentrations were mapped on chromosome 6 and 7. One QTL for shoot/root rate of Cd concentration was identified on chromosome 3. The results indicated that Cd tolerance and accumulation were quantitatively inherited, and the detected QTLs may be useful for marker-assistant selection (MAS) and identification of the genes controlling Cd tolerance and accumulation in rice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Arao T, Ae N (2003) Genotypic variation in cadmium levels of rice grain. Soil Sci Plant Nutr 49:473–479

    CAS  Google Scholar 

  • Chakravarty B, Srivastava S (1992) Toxicity of some heavy metals in vivo and in vitro in Helianthus annuus. Mutat Res 283:287–294

    Article  PubMed  CAS  Google Scholar 

  • Chen SL, Kao CH (1995) Cd induced changes in proline level and peroxidase activity in roots of rice seedlings. Plant Growth Regul 17:67–71

    CAS  Google Scholar 

  • Cheng WD, Zhang GP, Yao HG, Wu W, Xu M (2006) Genotypic and environmental variation in cadmium, chromium, arsenic, nickel and lead concentrations in rice grains. J Zhejiang Univ Sci B 7:565–571

    Article  PubMed  CAS  Google Scholar 

  • Dasgupta T, Hossain SA, Meharg AA, Price AH (2004) An arsenate tolerance gene on chromosome 6 of rice. New Phytol 163:45–49

    Article  CAS  Google Scholar 

  • Deniau AX, Pieper B, Bookum WT, Lindhout P, Aarts MGM, Schat H (2006) QTL analysis of cadmium and zinc accumulation in the heavy metal hyperaccumulator Thlaspi caerulescens. Theor Appl Genet 113:907–920

    Article  PubMed  CAS  Google Scholar 

  • Dong Y, Ogawa T, Lin D, Koh H, Kamiunten H, Matsuo M, Cheng S (2006) Molecular mapping of quantitative trait loci for zinc toxicity tolerance in rice seedling (Oryza sativa L.). Field Crops Res 95:420–425

    Article  Google Scholar 

  • Hassan MJ, Wang ZQ, Zhang GP (2005) Sulfur alleviates growth Inhibition and oxidative stress caused by cadmium toxicity in rice. J Plant Nutr 28:1785–1800

    Article  CAS  Google Scholar 

  • Hu YT, Kao CH (2003) Changes in protein and amino acid contents in two cultivars of rice seedlings with different apparent tolerance to cadmium. Plant Growth Regul 40:147–155

    Article  Google Scholar 

  • Ishikawa S, Ae N, Yano M (2005) Chromosomal regions with quantitative trait loci controlling cadmium concentration in brown rice (Oryza sativa). New Phytol 168(20):345–350

    Article  PubMed  CAS  Google Scholar 

  • Krupa Z (1988) Cadmium-induced changes in the composition and structure of the light-harvesting complex II in radish cotyledons. Physiol Plant 73:518–524

    Article  CAS  Google Scholar 

  • Lagerwerff JV (1972) Lead, mercury and cadmium as environmental contaminants. In: Mortvedt JJ, Giordano PM, Lindsay WL (eds) Micronutrient in agriculture. Soil Science and Society of America, Madison, WI, pp 593–636

    Google Scholar 

  • Larsson EH, Bordman JF, Asp H (1998) Influence of UV-B radiation and Cd2+ on chlorophyll fluorescence, growth and nutrient content in Brassica napus. J Exp Bot 49:1031–1039

    Article  CAS  Google Scholar 

  • Li ZK, Pinson SRM, Stansel JW, Park WD (1995) Identification of quantitative trait loci (QTL) for heading date and plant height in rice using RFLP markers. Theor Appl Genet 91:374–381

    CAS  Google Scholar 

  • Liu JG, Zhu QS, Zhang ZJ, Xu JK, Yang JC, Wong MH (2005) Variations in cadmium accumulation among rice cultivars and types and the selection of cultivars for reducing cadmium in the diet. J Sci Food Agric 85:147–153

    Article  CAS  Google Scholar 

  • Lu C, Shen L, Tan Z, Xu Y, He P, Chen Y, Zhu L (1996) Comparative mapping of QTLs for agronomic traits of rice across environments using a doubled haploid population. Theor Appl Genet 93:1211–1217

    Article  CAS  Google Scholar 

  • Ma JF, Shen R, Zhao Z, Wissuwa M, Takeuchi Y, Ebitani T, Yano M (2002) Response of rice to Al stress and identification of quantitative trait loci for Al tolerance. Plant Cell Physiol 43:652–659

    Article  PubMed  CAS  Google Scholar 

  • McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M (1997) Report on QTL nomenclature. Rice Genet Newsl 14:11–13

    Google Scholar 

  • Moya JL, Ros R, Picazo I (1993) Influence of cadmium and nickel on growth, net photosynthesis and carbohydrate distribution in rice plants. Photosynth Res 36:75–80

    Article  CAS  Google Scholar 

  • Nguyen VT, Burow MD, Nguyen HT, Le BT, Le TD, Paterson AH (2001) Molecular mapping of genes conferring aluminum tolerance in rice (Oryza sativa L.). Theor Appl Genet 102:1002–1010

    Article  CAS  Google Scholar 

  • Padmaja K, Prasad DDK, Prasad ARK (1990) Inhibition of chlorophyll synthesis in Phaseolus vulgaris seedlings by cadmium acetate. Photosynthetica 24:399–405

    CAS  Google Scholar 

  • Shao G, Hassan M, Zhang X, Zhang G (2004) Effects of cadmium stress on plant growth and antioxidative enzyme system in different rice genotype. Chin J Rice Sci 18:239–244 (in Chinese)

    CAS  Google Scholar 

  • Shao G, Chen M, Wang W, Mou R, Zhang G (2007) Iron nutrition affects cadmium accumulation and toxicity in rice plants. Plant Growth Regul 53:33–42

    Article  CAS  Google Scholar 

  • Siedlecka A, Baszynski T (1993) Inhibition of electron flow around photosystem I in chloroplasts of cadmium-treated maize plants in due to cadmium-induced iron deficiency. Physiol Plant 87:199–202

    Article  CAS  Google Scholar 

  • Stobart AK, Griffiths WT, Ameen-Bukhari I, Sherwood RP (1985) The effect of Cd2+ on the biosynthesis of chlorophyll of barley. Physiol Plant 63:293–298

    Article  CAS  Google Scholar 

  • Teng S, Qian Q, Zeng D, Kunihiro Y, Fujimoto K, Huang D, Zhu L (2004) QTL analysis of leaf photosynthetic rate and related physiological traits in rice (Oryza sativa L.). Euphytica 135:1–7

    Article  CAS  Google Scholar 

  • Verma S, Dubey RS (2001) Effect of cadmium on soluble sugars and enzymes of their metabolism in rice. Physiol Plant 44:117–123

    CAS  Google Scholar 

  • Wan J, Zhai H, Wan J, Ikehashi H (2003) Detection and analysis of QTLs ferrous iron toxicity tolerance in rice. Euphytica 131:201–206

    Article  CAS  Google Scholar 

  • Wang DL, Zhu J, Li ZK, Paterson AH (1999) Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet 99:1255–1264

    Article  Google Scholar 

  • Wang XY, Wu P, Wu YR, Yan XL (2002) Molecular marker analysis of manganese toxicity tolerance in rice under green house conditions. Plant Soil 238:227–233

    Article  CAS  Google Scholar 

  • Weigel HJ, Jäger HJ (1980) Subcellular distribution and chemical forms of cadmium in bean plants. Plant Physiol 65:480–482

    Article  PubMed  CAS  Google Scholar 

  • Williams CH, David DJ (1973) The effect of superphosphate on the cadmium content of soils and plants. Aust J Soil Res 11:43–56

    Article  CAS  Google Scholar 

  • Wong SC, Li XD, Zhang G, Qi SH, Min YS (2002) Heavy metals in agricultural soil of the Pearl River Delta, South China. Environ Pollut 119:33–44

    Article  PubMed  CAS  Google Scholar 

  • Wu P, Luo A, Zhu J, Yang J, Huang N, Senadhira D (1997) Molecular markers linked to genes underlying seedling tolerance for ferrous iron toxicity. Plant Soil 196:317–320

    Article  CAS  Google Scholar 

  • Wu P, Liao C, Hu B, Yi K, Jin W, Ni J, He C (2000) QTLs and epistasis for aluminum tolerance in rice (Oryza sativa L.) at different seedling stages. Theor Appl Genet 100:1295–1303

    Article  CAS  Google Scholar 

  • Wu F, Zhang G, Jia G, Zheng S (2006) Genotypic difference in the responses of germination and seedling growth to Cd toxicity in rice (Oryza sativa L.). Agric Sci China 5:68–75

    CAS  Google Scholar 

  • Xu YB, Shen LS, McCouch SR, Zhu LH (1998) Extension of the rice DH population genetic map with microsatellite markers. Chin Sci Bull 42:149–152

    Google Scholar 

  • Xu J, Li X, Zhu L (2004) Comparative mapping of rice root traits in seedlings grown in nutrient or non-nutrient solution. Prog Nat Sci 4:327–331

    Article  Google Scholar 

  • Xue Y, Wan JM, Jiang L, Liu LL, Su N, Zhai HQ, Ma JF (2006) QTL analysis of aluminum resistance in rice (Oryza sativa L.). Plant Soil 287:375–383

    Article  CAS  Google Scholar 

  • Yoshida S, Forna DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice. International Rice Research Institute, Los Banos, Philippines, pp 62–63

    Google Scholar 

  • Yu H, Wang J, Fang W, Yuan J, Yang Z (2006) Cadmium accumulation in different rice cultivars and screening for pollution-safe cultivars of rice. Sci Total Environ 370:302–309

    Article  PubMed  CAS  Google Scholar 

  • Zeng FR, Mao Y, Cheng WD, Wu FB, Zhang GP (2007) Genotypic and environmental variation in chromium, cadmium and lead concentrations in rice. Environ Pollut. doi:10.1016/j.envpol.2007.08.022

  • Zhang J, Zhu YG, Zeng DL, Cheng WD, Qian Q, Duan GL (2008) Mapping quantitative trait loci associated with arsenic accumulation in rice (Oryza sativa). New Phytol 177:350–356

    PubMed  CAS  Google Scholar 

  • Zhu LH, Chen Y, Xu YB, Xu JC, Cai HW, Ling ZZ (1993) Construction of a molecular map of rice and gene mapping using a double haploid population of a cross between Indica and Japonica varieties. Rice Genet Newsl 10:132–134

    Google Scholar 

Download references

Acknowledgement

We are very grateful to Zhejiang Natural Science Foundation (Z304104) for their financial support.

Author information

Authors and Affiliations

  1. Agronomy Department, Zhejiang University, 310029, Hangzhou, China

    Dawei Xue & Guoping Zhang

  2. College of Agronomy, Henan University of Science and Technology, 471003, Luoyang, China

    Mingcan Chen

Authors
  1. Dawei Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mingcan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guoping Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guoping Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xue, D., Chen, M. & Zhang, G. Mapping of QTLs associated with cadmium tolerance and accumulation during seedling stage in rice (Oryza sativa L.). Euphytica 165, 587–596 (2009). https://doi.org/10.1007/s10681-008-9785-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-008-9785-3

Keywords

Search

Navigation