Skip to main content
SpringerLink
Log in

Characterization and pathogenicity of sweet potato (Ipomoea batatas) black rot caused by Ceratocystis fimbriata in Korea

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Black rot disease was observed in sweet potato in Korea during a disease monitoring survey in 2017. The symptoms were black, small, circular, and slightly sunken lesions in the initial stages, leading to enlarged black spots and perithecia with long necks that appeared as dark bristles in the later stages. The causative agent was isolated on potato dextrose agar (PDA) and stored at the Sweet Potato Research Laboratory, Bioenergy Crop Research Institute, RDA, Muan (SPL17100 and SPL17101). The cultured organism produced cylindrical single-celled conidia after 5 days of incubation at 25 °C with a conidial size of 8.5–65.2 × 2.6–7.3 μm. Brownish, globose, and thick-walled aleuroconidia were produced with an 11.9–17.0 × 8.3–12.2 μm size. The perithecia were globose and dark brown with long thin necks tapering from the base to the apex. Morphologically, the fungus was identified as Ceratocystis fimbriata, which was well supported by the combined phylogenetic analysis of the sequences of the internal transcribed spacer (ITS), large subunit (LSU), and elongation factor 1-α (EF-1α) genes. Pathogenicity tests were conducted, and Koch’s postulates were confirmed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Bakar, C. J. E., & Harrington, T. C. (2005). Intersterility, morphology and taxonomy of Ceratocystis fimbriata on sweet potato cacao and sycamore. Mycologia, 97, 57–69.

    Article  Google Scholar 

  • Chen, S., Wyk, M. V., Roux, J., Wingfield, M. J., Xie, Y. J., & Zhou, X. D. (2013). Taxonomy and pathogenicity of Ceratocystis species on Eucalyptus trees in South China, including C. chinaeucensis sp. nov. Fungal Diversity, 58, 267–279.

    Article  Google Scholar 

  • Cho, W. D., & Shin, H. D. (2004). List of plant diseases in Korea. Korean Society of Plant Pathology: Seoul, Republic of Korea.

    Google Scholar 

  • Clark, C. A., Ferrin, D. M., Smith, T. P., & Holmes, G. J. (2013). Compendium of sweet potato diseases, pests, and disorders. St. Paul: APS press.

    Book  Google Scholar 

  • Clark, C. A. (1980). End rot, surface rot, and stem lesions caused on sweet potato by Fusarium solani. Phytopathology, 70, 109–112.

    Article  Google Scholar 

  • Hall, T. A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–108.

    CAS  Google Scholar 

  • Halsted, B. D., & Fairchild, D. G. (1891). Sweet potato black rot. Journal of Mycology, 7, 1–11.

    Article  Google Scholar 

  • Lee, D. H., Roux, J., Wingfield, B. D., Barnes, I., & Wingfield, M. J. (2016). New host range and distribution of Ceratocystis pirilliformis in South Africa. European Journal of Plant Pathology, 146, 483–496.

    Article  CAS  Google Scholar 

  • Li, Q., Harrington, T. C., McNew, D., Li, J., Huang, Q., Somasekhara, Y. M., & Alfenas, A. C. (2016). Genetic bottlenecks for two populations of Ceratocystis fimbriata on sweet potato and pomegranate in China. Plant Disease, 100, 2266–2274.

    Article  PubMed  Google Scholar 

  • Loebenstein, G., & Thottappilly, G. (2009). The sweet potato. The Netherlands, Springer Science + Business Media BV.

  • Muramoto, N., Tanaka, T., Shimamura, T., Mitsukawa, N., Hori, E., Koda, K., Otani, M., Hirai, M., Nakamura, K., & Imaeda, T. (2012). Transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by Ceratocystis fimbriata in leaves and storage roots. Plant Cell Reports, 31, 987–997.

    Article  CAS  PubMed  Google Scholar 

  • Nkuekam, G. K., Wingfield, M. J., & Roux, J. (2013). Ceratocystis species, including two new taxa, from Eucalyptus trees in South Africa. Australasian Plant Pathology, 42, 283–311.

    Article  Google Scholar 

  • O’Donnel, K. (2000). Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia, 92, 919–938.

    Article  Google Scholar 

  • Paul, N. C., Lee, H. B., Lee, J. H., Shin, K. S., Ryu, T. H., Kwon, H. R., Kim, Y. K., Youn, Y. N., & Yu, S. H. (2014). Endophytic fungi from Lycium chinense Mill and characterization of two new Korean records of Colletotrichum. International Journal of Molecular Sciences, 15, 15272–15286.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Roux, J., Wingfield, M. J., Bouillet, J. P., Wingfield, B. D., & Alfenas, A. C. (2000). A serious new wilt disease of Eucalyptus caused by Ceratocystis fimbriata in Central Africa. Forest Pathology, 30, 175–184.

    Article  Google Scholar 

  • Surak, J. G. (1978). Phytoalexins and human health-a review. Proceedings of the Florida State Horticultural Society, 91, 256–258.

    CAS  Google Scholar 

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., & Higgins, D. G. (1997). ClustalX: Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 487–488.

    Article  Google Scholar 

  • Valdetaro, D. C. O. F., Oliveira, L. S. S., Guimaraes, L. M. S., Harrington, T. C., Ferreira, M. A., Freitas, R. G., & Alfenas, A. C. (2015). Genetic variation, morphology and pathogenicity of Ceratocystis fimbriata on Hevea brasiliensis in Brazil. Tropical Plant Pathology, 40, 184–192.

    Article  Google Scholar 

  • Vilgalys, R., & Hester, M. (1990). Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology, 172, 4238–4246.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wamalwa, L. N., Cheseto, X., Ouna, E., Kaplan, F., Maniania, N. K., Machuka, J., Torto, B., & Ghislain, M. (2015). Toxic ipomeamarone accumulation in healthy parts of sweetpotato (Ipomoea batatas L. Lam) storage roots upon infection by Rhizopus stolonifer. Journal of Agricultural and Food Chemistry, 63, 335–342.

    Article  CAS  PubMed  Google Scholar 

  • White, T. J., Bruns, T. D., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). New York: Academic Press.

    Google Scholar 

Download references

Acknowledgements

This research was supported by the Basic Research Program (Project No. PJ01207601) funded by the Rural Development Administration, Republic of Korea.

Author information

Authors and Affiliations

  1. Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan, 58545, Republic of Korea

    Narayan Chandra Paul, Sang-Sik Nam & Jung-Wook Yang

  2. Department of Plant Pathology, University of Kentucky, Lexington, KY, USA

    Aardra Kachroo

  3. Department of Biology Education, IALS, Gyeongsang National University, Jinju, South Korea

    Yun-Hee Kim

Authors
  1. Narayan Chandra Paul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sang-Sik Nam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aardra Kachroo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yun-Hee Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jung-Wook Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jung-Wook Yang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal studies

No human participants and/or animals were involved in this research content.

Informed consent

This manuscript has not been published or presented elsewhere in part or in entirety and is not under consideration by another journal. We have read and understood your journal policies, and we believe that neither the manuscript nor the study violates any of these. All the authors have been personally and actively involved in substantive work leading to the manuscript and will hold themselves jointly and individually responsible for its content. All co-authors agreed to submit the article to the ‘European Journal of Plant Pathology’.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Paul, N.C., Nam, SS., Kachroo, A. et al. Characterization and pathogenicity of sweet potato (Ipomoea batatas) black rot caused by Ceratocystis fimbriata in Korea. Eur J Plant Pathol 152, 833–840 (2018). https://doi.org/10.1007/s10658-018-1522-8

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-018-1522-8

Keywords

Search

Navigation