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The effect of magnetic bead size on the isolation efficiency of lung cancer cells in a serpentine microchannel with added cavities

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Abstract

An investigation was conducted to examine the effect of magnetic bead (MB) size on the effectiveness of isolating lung cancer cells using the immunomagnetic separation (IMS) method in a serpentine microchannel with added cavities (SMAC) structure. Carboxylated magnetic beads were specifically conjugated to target cells through a modification procedure using aptamer materials. Cells immobilized with different sizes (in micrometers) of MBs were captured and isolated in the proposed device for comparison and analysis. The study yields significance regarding the clarification of device working principles by using a computational model. Furthermore, an accurate evaluation of the MB size impact on capture efficiency was achieved, including the issue of MB-cell accumulation at the inlet-channel interface, despite it being overlooked in many previous studies. As a result, our findings demonstrated an increasing trend in binding efficiency as the MB size decreased, evidenced by coverages of 50.5%, 60.1%, and 73.4% for sizes of 1.36 μm, 3.00 μm, and 4.50 μm, respectively. Additionally, the overall capture efficiency (without considering the inlet accumulation) was also higher for smaller MBs. However, when accounting for the actual number of cells entering the channel (i.e., the effective capture), larger MBs showed higher capture efficiency. The highest effective capture achieved was 88.4% for the size of 4.50 μm. This research provides an extensive insight into the impact of MB size on the performance of IMS-based devices and holds promise for the efficient separation of circulating cancer cells (CTCs) in practical applications.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This work is funded by the Ministry of Science and Technology of the Republic of China (Taiwan) through the grant number of MOST 109-2923-E-194-001-MY3 as well as well as Changhua Christian Hospital (110-CCH-MST-142) in Taiwan.

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Authors and Affiliations

  1. Department of Surgical Pathology, Changhua Christian Hospital, Changhua, 500, Taiwan, R.O.C.

    Tzu-Cheng Su & Shu-Hui Lin

  2. School of Medicine, Chung Shan Medical University, Taichung, 402, Taiwan, R.O.C.

    Tzu-Cheng Su

  3. Department of Mechanical Engineering and Advanced Institute of Manufacturing for High-Tech Innovations, National Chung Cheng University, Chia-Yi, 62102, Taiwan, R.O.C.

    Hien Vu-Dinh & Chun-Ping Jen

  4. Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, 402, Taiwan, R.O.C.

    Shu-Hui Lin

  5. Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung, 402, Taiwan, R.O.C.

    Shu-Hui Lin

  6. Faculty of Physics, University of Science, Vietnam National University, Hanoi, 100000, Vietnam

    Loc Do Quang

  7. Faculty of Electronics and Telecommunication, University of Engineering and Technology, Vietnam National University, Hanoi, 100000, Vietnam

    Trinh Chu Duc

  8. School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan, R.O.C.

    Chun-Ping Jen

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  1. Tzu-Cheng Su
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Contributions

T-C. S. and H. V.-D. wrote the main manuscript text; H. V.-D. and S.-H. L. curated the experimental data; L. D. Q. performed the numerical results; T. C. D. and C.P. J. reviewed the manuscript; C.-P. J. supervised and validated the design and concept of this work. All authors reviewed the manuscript.

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Correspondence to Chun-Ping Jen.

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Su, TC., Vu-Dinh, H., Lin, SH. et al. The effect of magnetic bead size on the isolation efficiency of lung cancer cells in a serpentine microchannel with added cavities. Biomed Microdevices 26, 7 (2024). https://doi.org/10.1007/s10544-023-00689-5

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