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Effect of Pb ions on superoxide dismutase and catalase activities in leaves of pea plants grown in high and low irradiance

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Biologia Plantarum

Abstract

The role of irradiance on the activity of antioxidant enzymes: superoxide dismutase (SOD) and catalase (CAT) was examined in the leaves of Pisum sativum L. plants grown under low (LL) or high (HL) irradiance (PPFD 50 or 600 µmol m−2 s−1) and exposed after detachment to 5 mM Pb (NO3)2 for 24 h. The activities of both enzymes increased in response to LL compared with HL and no effect of Pb ions was observed. Photosystem (PS) 1 and PS 2 activities were also investigated in chloroplasts isolated from these leaves. LL lowered PS 1 electron transport rate and changes in photochemical activity of PS 1 induced by Pb2+ were visible only in the chloroplasts isolated from leaves of LL grown plants. PS 2 activity was influenced similarly by Pb ions at both PPFD. This study demonstrates that leaves of HL grown plants were less sensitive to lead toxicity than those from LL grown plants. Changes in electron transport rates were the main factors responsible for the generation of reactive oxygen species in the chloroplasts and as a consequence, in induction of antioxidant enzymes.

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Abbreviations

CAT:

catalase

DBMIB:

2,5-dibromo-3-methyl-6-isopropyl-1,4-benzoquinone

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

HL:

high irradiance

LL:

low irradiance

MV:

methylviologen

NBT:

nitroblue tetrazolium

OEC:

oxygen evolving complex

PBQ:

phenyl-p-benzoquinone

PPFD:

photosynthetic photon flux density

PS 1 and PS 2:

photosystem 1 and 2

ROS:

reactive oxygen species

SOD:

superoxide dismutase

TEMED:

tetramethylethylenediamine

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Authors and Affiliations

  1. Department of Plant Physiology, Institute of Botany, University of Warsaw, Miecznikowa 1, PL-02096, Warsaw, Poland

    E. Romanowska, B. Wróblewska, A. Drożak, M. Zienkiewicz & M. Siedlecka

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  1. E. Romanowska
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  2. B. Wróblewska
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  3. A. Drożak
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  4. M. Zienkiewicz
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  5. M. Siedlecka
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Correspondence to E. Romanowska.

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Romanowska, E., Wróblewska, B., Drożak, A. et al. Effect of Pb ions on superoxide dismutase and catalase activities in leaves of pea plants grown in high and low irradiance. Biol Plant 52, 80–86 (2008). https://doi.org/10.1007/s10535-008-0012-9

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  • DOI: https://doi.org/10.1007/s10535-008-0012-9

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