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Degradation characteristics of crude oil by a consortium of bacteria in the existence of chlorophenol

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Abstract

In order to enhance the degradation effect of microorganisms on crude oil in the existence of chlorophenol compounds, oil-degrading bacteria C4 (Alcaligenes faecails), C5 (Bacillus sp.) and 2,4-dichlorophenol (2,4-DCP) degrading bacteria L3 (Bacillus marisflavi), L4 (Bacillus aquimaris) were isolated to construct a highly efficient consortium named (C4C5 + L3L4). When the compound bacteria agent combination by VC4: VC5: VL3: VL4 = 1:2:2:1, the crude oil degradation efficiency of 7 days was stable at 50.63% ~ 55.43% under different conditions. Degradation mechanism was analyzed by FTIR, GC–MS and IC technology and the following conclusions showed that in the system of adding consortium (C4C5 + L3L4), the heavy components were converted into saturated and unsaturated components. The bacterial consortium could first degrade medium and long chain alkanes into short chain hydrocarbons and then further degrade. And the dechlorination efficiency of 2,4-DCP in the degradation system reached 73.83%. The results suggested that the potential applicability and effectiveness of the selected bacteria consortium for the remediation of oil-contaminated water or soil with the existence of chlorophenol compound.

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The datasets and materials were used and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors sincerely thank the grant funded by the State Key Laboratory of Petroleum Pollution Control.

Funding

This study was supported by the Independent Project Program of State Key Laboratory of Petroleum Pollution Control (Grant No. PPC2018014), CNPC Research Institute of Safety and Environmental Technology.

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  1. Jing Li and Qiyou Liu have contributed equally to this work.

Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, China University of Petroleum (East China), No. 66, Changjiang West Road, Huangdao District, Qingdao, 266580, China

    Jing Li, Qiyou Liu, Shuo Sun, Xiuxia Zhang, Xiuying Zhao, Junlong Yu, Wu Cui & Yi Du

  2. State Key Laboratory of Petroleum Pollution Control, Qingdao, 266580, China

    Jing Li, Qiyou Liu, Shuo Sun & Xiuxia Zhang

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  1. Jing Li
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Contributions

All authors contributed to the design of the research. JL and QL carried out research methods, data analysis and first draft writing, as the co-first author. SS, XZ, XZ, JY, WC, YD participated in research methods, data analysis, revision of manuscripts.

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Correspondence to Qiyou Liu.

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Li, J., Liu, Q., Sun, S. et al. Degradation characteristics of crude oil by a consortium of bacteria in the existence of chlorophenol. Biodegradation 33, 461–476 (2022). https://doi.org/10.1007/s10532-022-09992-z

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