Abstract
Purpose
Erythritol is a valuable compound as sweetener and chemical material however cannot be fermented from the abundant substrate xylose.
Methods
The strain Trichosporonoides oedocephalis ATCC 16958 was employed to produce polyols including xylitol and erythritol by metabolic engineering approaches.
Results
The introduction of a substrate-specific ribose-5-phosphate isomerase endowed T. oedocephalis with xylose-assimilation activity to produce xylitol, and eliminated glycerol production simultaneously. A more value-added product, erythritol was produced by further introducing a homologous xylulose kinase. The carbon flux was redirected from xylitol to erythritol by adding high osmotic pressure. The production of erythritol was improved to 46.5 g/L in flasks by fermentation adjustment, and the process was scaled up in a 5-L fermentor, with a 40 g/L erythritol production after 120 h, and a time–space yield of 0.56 g/L/h.
Conclusion
This study demonstrated the potential of T. oedocephalis in the synthesis of multiple useful products from xylose.
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Acknowledgements
The authors are grateful for the support from the National Natural Science Foundation of China (Grant no. 21676173), the Agricultural Infrastructure Project of Suzhou Science and Technology Development Plan (Grant no. SNG2022057) and the Graduate Research and Innovation Projects of Jiangsu Province (Grant no. KYCX21_3042).
Supplementary data
Table S1—Strains and plasmids used in this study.
Table S2—Primers and sequences used in this study.
Table S3—Comparison of erythritol production by strains with different carbon sources.
Table S4—Comparison of xylitol production by strains with different carbon sources.
Table S5—Summary of fermentation with xylose and glucose.
Figure S1—Construction of expression vectors. A) agarose gel analysis of GAL1, B) OsRpiB, C) xylulose kinase, and D) xylitol dehydrogenase genes; E) plasmid diagram of three vectors that contain OsRpiB, OsRpiB+ xylulose kinase, and OsRpiB+ xylulose kinase+ xylitol dehydrogenase genes respectively.
Figure S2—HPLC diagram of product analysis. A) TOR (OsRpiB containing strain) fermentation with xylose; B) TORKD fermentation with xylose; C) TORKD xylose-glucose cofermentation; the retention time of glucose, xylose, erythritol and xylitol is 15.7 min, 17.5 min, 23.3 min and 26.9 min.
Figure S3—HPLC diagram of product purity under different fermentation conditions.
Figure S4—Time courses of parameters in xylose concentration comparison. A) erythritol production, B) xylitol production, and C) variation of pH.
Funding
National Natural Science Foundation (Grant no. 21676173).
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ZD and YM: Investigation, Writing—original draft. ZC and LY: Writing—review & editing. XJ: Methodology, Supervision. LL: Funding acquisition, Conceptualization, Methodology, Writing—review & editing, Supervision.
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Deng, Z., Mu, Y., Chen, Z. et al. Construction of a xylose metabolic pathway in Trichosporonoides oedocephalis ATCC 16958 for the production of erythritol and xylitol. Biotechnol Lett 45, 1529–1539 (2023). https://doi.org/10.1007/s10529-023-03428-1
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DOI: https://doi.org/10.1007/s10529-023-03428-1