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miR-1286, a Tumor Suppressor of Gastric Cancer, Serves as a Promising Biomarker for Screening Gastric Cancer from Gastritis

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Abstract

Gastric cancer (GC) is one of the crucial causes of cancer-associated death worldwide. This study aimed to investigate the biological function of miR-1286 in GC progression in vitro, evaluate the clinical value of serum miR-1286 to screen GC patients and explore its relationship with helicobacter pylori (HP) infection and peritoneal metastasis in GC patients. Expression of miR-1286 was measured by RT-qPCR. Cell Counting Kit-8 assay was utilized for measuring GC cell proliferation ability. The migration and invasion abilities of GC cells were measured using Transwell assays. Serum samples were obtained from 108 GC patients, 62 gastritis cases and 62 healthy volunteers. The diagnostic performance of miR-1286 was assessed using ROC analysis, and the predictive value of miR-1286 for peritoneal metastasis onset was analyzed using logistic regression analysis. miR-1286 played as a tumor suppressor in GC progression by inhibiting GC cell proliferation, migration and invasion. In GC patients, significantly decreased miR-1286 was observed compared to gastritis and healthy controls, and had considerable diagnostic accuracy to distinguish GC from the controls. A significant association was found between miR-1286 expression and HP infection, peritoneal metastasis and TNM stage. Moreover, miR-1286 was lowly expressed in GC patients with peritoneal metastasis, and independently predicted the occurrence of peritoneal metastasis in GC. miR-1286 acts as a tumor suppressor and a biomarker in GC, and is closely associated with HP infection and peritoneal metastasis onset. The methods to regulate miR-1286 may be novel strategies to improve the treatment of GC.

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Data Availability

The data used to support the findings of this study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Laboratory, Huantai County People’s Hospital, Zibo, 256400, Shandong, China

    Min Tian

  2. Department of General Surgery, Peking University Care Luzhong Hospital, Zibo, 255499, Shandong, China

    Meiquan Jiang

  3. Department of Gastroenterology, Peking University Care Luzhong Hospital, Zibo, 255499, Shandong, China

    Yingjie Bi

  4. Department of Otolaryngology Head and Neck Surgery, Peking University Care Luzhong Hospital, No. 65, Linyi District, Zibo, 255499, Shandong, China

    Bing Wang

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  1. Min Tian
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  2. Meiquan Jiang
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  3. Yingjie Bi
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  4. Bing Wang
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Contributions

MT carried out the research design and conception; MJ and YB analyzed and interpreted the data regarding; MJ and BW performed the examination of sample; YB contributed essential reagents or tools; MT and BW authors wrote and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Bing Wang.

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The authors declare that they have no competing interests.

Ethics Approval and Consent to Participate

The experimental procedures were all in accordance with the guideline of the Ethics Committee of Huantai County People’s Hospital and has approved by the Ethics Committee of Huantai County People’s Hospital. A signed written informed consent was obtained from each patient.

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Written informed consent for publication was obtained from each participant.

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Tian, M., Jiang, M., Bi, Y. et al. miR-1286, a Tumor Suppressor of Gastric Cancer, Serves as a Promising Biomarker for Screening Gastric Cancer from Gastritis. Biochem Genet (2024). https://doi.org/10.1007/s10528-023-10618-z

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  • DOI: https://doi.org/10.1007/s10528-023-10618-z

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