Skip to main content

Advertisement

SpringerLink
Log in

Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori

  • Original Research
  • Published:
Journal of Gastrointestinal Cancer Aims and scope Submit manuscript

Abstract

Purpose

The aim of this study was to evaluate the expression of miR-125a-5p in patients with dyspeptic symptoms and gastric cancer, correlating them with the development of this cancer and H. pylori.

Methods

Patients were divided in groups according to histopathological analysis (control, gastritis, and cancer groups). Polymerase chain reaction was performed to detect H. pylori and real-time quantitative PCR to determine miR-125a-5p expression.

Results

H. pylori was detected in 44% of the patients, with prevalence in the gastritis and cancer groups. A statistically significant decrease of miR-125a-5p expression was found in the control positive (p = 0.0183*), gastritis positive (p = 0.0380*), and cancer positive (p = 0.0288*) groups when compared with the control negative group.

Conclusion

We suggest that decreased expression of the miRNA-125a-5p associated with the presence of the H. pylori is an important mechanism in gastric diseases and could be a possible marker for early diagnosis of gastric cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Strong VE. Progress in gastric cancer. Updat Surg. 2018;70:157–9. https://doi.org/10.1007/s13304-018-0543-3 [Internet]. Springer International Publishing.

    Article  Google Scholar 

  2. Cai M, Chen Q, Shen J, Lv C, Cai L. Epigenetic silenced miR-125a-5p could be self-activated through targeting Suv39h1 in gastric cancer. J Cell Mol Med. 2018;22:4721–31. https://doi.org/10.1111/jcmm.13716.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Pei Y, Tang Z, Cai M, Yao Q, Xie B, Zhang X. The E2F3/miR-125a/DKK3 regulatory axis promotes the development and progression of gastric cancer. Cancer Cell Int. 2019;19:1–12. https://doi.org/10.1186/s12935-019-0930-y [Internet]. BioMed Central.

    Article  CAS  Google Scholar 

  4. Zhang Z, Dong Y, Hua J, Xue H, Hu J, Jiang T, et al. A five-miRNA signature predicts survival in gastric cancer using bioinformatics analysis. Gene. 2019;699:125–34. https://doi.org/10.1016/j.gene.2019.02.058 [Internet]. Elsevier B.V.

    Article  CAS  PubMed  Google Scholar 

  5. Sabbagh P, Javanian M, Koppolu V, Vasigala VKR, Ebrahimpour S. Helicobacter pylori infection in children: an overview of diagnostic methods. Eur J Clin Microbiol Infect Dis. 2019;38:1035–45. https://doi.org/10.1007/s10096-019-03502-5.

    Article  CAS  PubMed  Google Scholar 

  6. Cao Y, Tan S, Tu Y, Zhang G, Liu Y, Li D, et al. MicroRNA-125a-5p inhibits invasion and metastasis of gastric cancer cells by targeting BRMS1 expression. Oncol Lett. 2018;15:5119–30. https://doi.org/10.3892/ol.2018.7983.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Sonkoly E, Pivarcsi A. Advances in microRNAs: implications for immunity and inflammatory diseases. J Cell Mol Med. 2009;13:24–38. https://doi.org/10.1111/j.1582-4934.2008.00534.x.

  8. Rossi AFT, Cadamuro ACT, Biselli-Périco JM, Leite KRM, Severino FE, Reis PP, et al. Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection. Cell Microbiol. 2016;18:1444-58. https://doi.org/10.1111/cmi.12587.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Noto JM, Peek RM. The role of microRNAs in Helicobacter pylori pathogenesis and gastric carcinogenesis. Front Cell Infect Microbiol. 2012;1:1-19. https://doi.org/10.3389/fcimb.2011.00021.

    Article  CAS  Google Scholar 

  10. Hashiguchi Y, Nishida N, Mimori K, Sudo T, Tanaka F, Shibata K, et al. Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance. Int J Oncol. 2012;40:1477-82. https://doi.org/10.3892/ijo.2012.1363.

    Article  CAS  PubMed  Google Scholar 

  11. Hou L, Luo P, Ma Y, Jia C, Yu F, Lv Z, et al. MicroRNA-125a-3p downregulation correlates with tumorigenesis and poor prognosis in patients with non-small cell lung cancer. Oncol Lett. 2017;14:4441-8. https://doi.org/10.3892/ol.2017.6809.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Stolte M, Meining A. The updated Sydney system: classification and grading of gastritis as the basis of diagnosis and treatment. Can J Gastroenterol. 2001;15:591-8. https://doi.org/10.1155/2001/367832.

    Article  CAS  PubMed  Google Scholar 

  13. Scholte GH, van Doorn LJ, Quint WG, Lindeman J. Polymerase chain reaction for the detection of Helicobacter pylori in formaldehyde-sublimate fixed, paraffin-embedded gastric biopsies. Diagn Mol Pathol. 1997;6:238-43. https://doi.org/10.1097/00019606-199708000-00008.

    Article  CAS  PubMed  Google Scholar 

  14. Santos M, Sallas L, Zapparoli D, Orcini WA, Chen E, Smith M, et al. Lack of association between IL-6 polymorphisms and haplotypes with gastric cancer. 2018;120:9448-54. https://doi.org/10.1002/jcb.28220.

  15. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 2001;25:402-8. https://doi.org/10.1006/met.2001.1262.

    Article  CAS  PubMed  Google Scholar 

  16. Ishimoto T, Baba H, Izumi D, Sugihara H, Kurashige J, Iwatsuki M, et al. Current perspectives toward the identification of key players in gastric cancer microRNA dysregulation. Int J Cancer. 2016;138:1337-49. https://doi.org/10.1002/ijc.29627.

    Article  CAS  PubMed  Google Scholar 

  17. Sallas ML, Melchiades JL, Zabaglia LM, do Prado Moreno JR, Orcini WA, Chen ES, et al. Prevalence of Helicobacter pylori vacA, cagA, dupA and oipA genotypes in patients with gastric disease. Adv Microbiol. 2017;07:1-9. https://doi.org/10.4236/aim.2017.71001.

    Article  CAS  Google Scholar 

  18. Wang F, Meng W, Wang B, Qiao L. Helicobacter pylori-induced gastric inflammation and gastric cancer. Cancer Lett. 2014;345:196-202. https://doi.org/10.1016/j.canlet.2013.08.016 [Internet]. Elsevier Ireland Ltd.

    Article  CAS  PubMed  Google Scholar 

  19. Yamaoka Y. Roles of the plasticity regions of Helicobacter pylori in gastroduodenal pathogenesis. J Med Microbiol. 2008;57:545-53. https://doi.org/10.1099/jmm.0.2008/000570-0.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Zheng Q, Chen C, Guan H, Kang W, Yu C. Prognostic role of microRNAs in human gastrointestinal cancer: a systematic review and meta-analysis. Oncotarget. 2017;8:46611-23. https://doi.org/10.18632/oncotarget.16679.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Zhong L, Sun S, Shi J, Cao F, Han X, Chen Z. MicroRNA-125a-5p plays a role as a tumor suppressor in lung carcinoma cells by directly targeting STAT3. Tumor Biol. 2017;39:101042831769757. https://doi.org/10.1177/1010428317697579.

    Article  CAS  Google Scholar 

  22. Park Y, Kim J. Regulation of IL-6 signaling by miR-125a and let-7e in endothelial cells controls vasculogenic mimicry formation of breast cancer cells. BMB Rep. 2019;52:214-9. https://doi.org/10.5483/BMBRep.2019.52.3.308.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Wu WY, Xue XY, Chen ZJ, Han SL, Huang YP, Zhang LF, et al. Potentially predictive microRNAs of gastric cancer with metastasis to lymph node. World J Gastroenterol. 2011;17:3645-51. https://doi.org/10.3748/wjg.v17.i31.3645.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Wang JK, Wang Z, Li G. MicroRNA-125 in immunity and cancer. Cancer Lett. 2019;454:134-45. https://doi.org/10.1016/j.canlet.2019.04.015 [Internet]. Elsevier.

    Article  CAS  PubMed  Google Scholar 

  25. Dai J, Wang J, Yang L, Xiao Y, Ruan Q. MIR-125a regulates angiogenesis of gastric cancer by targeting vascular endothelial growth factor A. Int J Oncol. 2015;47:1801-10. https://doi.org/10.3892/ijo.2015.3171.

    Article  CAS  PubMed  Google Scholar 

  26. Jo HN, Kang H, Lee A, Choi J, Chang W, Lee MS, et al. Endothelial miR-26a regulates VEGF-Nogo-B receptor-mediated angiogenesis. BMB Rep. 2017;50:384-9. https://doi.org/10.5483/bmbrep.2017.50.7.085.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Lu G, Ma Y, Jia C, Yang H, Xie R, Luo P, et al. Reduced miR-125a levels associated with poor survival of patients with hepatocellular cancer. Oncol Lett. 2017;14:5952-8. https://doi.org/10.3892/ol.2017.6902.

  28. Sun M, Guo S, Yao J, Xiao Y, Sun R, Ma W, et al. MicroRNA-125a suppresses cell migration, invasion, and regulates hyaluronic acid synthase 1 expression by targeting signal transducers and activators of transcription 3 in renal cell carcinoma cells. J Cell Biochem. 2019;120:1894-902. https://doi.org/10.1002/jcb.27503.

    Article  CAS  PubMed  Google Scholar 

  29. Kiselev FL. MicroRNA and cancer. Mol Biol (Mosk). 2014;48:232-42. https://doi.org/10.1016/j.molonc.2012.09.006 [Internet]. Elsevier B.V.

    Article  CAS  Google Scholar 

Download references

Funding

The authors would like to thank the São Paulo Research Foundation (FAPESP) grant nos. 2018/08481-1 and 2018/02008-2 for the financial support.

Author information

Authors and Affiliations

  1. Genetics Laboratory, Marília Medical School (FAMEMA), Lourival Freire, 240, Bairro Fragata, Marília, São Paulo, CEP 17519-050, Brazil

    Mônica Pezenatto dos Santos, Jéssica Nunes Pereira, Roger Willian De Labio & Spencer Luíz Marques Payão

  2. Institute of Tropical Pathology and Public Health, Federal University of Goiás (UFG), Goiânia, Goiás, Brazil

    Lilian Carla Carneiro, Jaqueline Correia Pontes & Mônica Santiago Barbosa

  3. Morphology Department, Federal University of Sao Paulo (UNIFESP), São Paulo, Brazil

    Marília De Arruda Cardoso Smith

  4. Biochemistry Department, Marília Medical School (FAMEMA), Lourival Freire, 240, Bairro Fragata, Marília, São Paulo, CEP 17519-050, Brazil

    Lucas Trevizani Rasmussen

Authors
  1. Mônica Pezenatto dos Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jéssica Nunes Pereira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Roger Willian De Labio
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lilian Carla Carneiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jaqueline Correia Pontes
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mônica Santiago Barbosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marília De Arruda Cardoso Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Spencer Luíz Marques Payão
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Lucas Trevizani Rasmussen
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Conceptualization, Mônica Santos and Lucas Rasmussen; data curation, Lucas Rasmussen; formal analysis, Mônica Santos; funding acquisition, Mônica Santos and Lucas Rasmussen; investigation, Mônica Santos and Jéssica Pereira; methodology, Mônica Santos; project administration, Lucas Rasmussen; resources, Lilian Carneiro, Jaqueline Pontes, Mônica Santiago, Marília Smith, and Spencer Payao; software, Mônica Santos and Lucas Rasmussen; supervision, Roger De Labio; validation, Spencer Payao, and Lucas Rasmussen; visualization, Lucas Rasmussen; writing (review and editing), Mônica Santos and Lucas Rasmussen. All authors have read and agreed with the published version of the manuscript.

Corresponding author

Correspondence to Lucas Trevizani Rasmussen.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

Approved by the Ethics and Research Committee of Sagrado Coração University, located in Bauru, State of São Paulo, Brazil (case number 1.119.830), and all subjects signed the consent form.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

dos Santos, M.P., Pereira, J.N., De Labio, R.W. et al. Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori. J Gastrointest Canc 52, 569–574 (2021). https://doi.org/10.1007/s12029-020-00432-w

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12029-020-00432-w

Keywords

Search

Navigation