Abstract
Aleuroglyphus ovatus (Acari: Acaridae) is a major pest mite of stored grains that is distributed worldwide. Paeonol, a phenolic component of the essential oil extracted from the Chinese herb Paeonia moutan, possesses a range of biological activities, including antiviral, antifungal and acaricidal activity. This study investigated the bioactivity of paeonol against A. ovatus and its effect on the activity of detoxification enzymes. The bioactivity of paeonol against A. ovatus was determined by contact, fumigation and repellency bioassays, and the mechanism was preliminarily explored via morphological observation of the color changes of mite epidermis and determination of the changing trend of some important enzymes associated with acaricidal efficacy in the mites. The results showed that the median lethal concentration (LC50) in the contact and fumigation bioassays was 9.832 μg/cm2 and 14.827 μg/cm3, respectively, and the acaricidal activity of paeonol was higher under direct contact than under fumigation. Dynamic symptomatology studies registered typical neurotoxicity symptoms including excitation, convulsion and paralysis in A. ovatus treated with paeonol. The enzyme activity of catalase (CAT), nitric oxide synthase (NOS) and glutathione-S-transferase (GST) was higher, whereas the activity of superoxide dismutase (SOD) and acetylcholinesterase (AChE) was lower, compared to the control group. CAT, NOS and GST were activated, whereas SOD and AChE activities were inhibited after paeonol intervention. Our findings suggest paeonol has potent acaricidal activity against A. ovatus and thus may be used as an agent to control the stored-product mite A. ovatus.
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The datasets generated and/or analysed during the current study are available from the corresponding author on reasonable request.
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This work was funded by a grant from the Academic Aid Program for Top-notch Talents in Provincial Universities (gxbjZD2020071) and the Wuhu Key Research and Development Program of China (2021yf39).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by MZ, QX, QT, QZ, TL, YL and JZ. The first draft of the manuscript was written by MZ and all authors commented on previous versions of the manuscript. YL and JZ read and approved the final manuscript.
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This study was performed in accordance with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Wannan Medical College.
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Zou, M., Xue, Q., Teng, Q. et al. Acaricidal activities of paeonol from Moutan Cortex, dried bark of Paeonia × suffruticosa, against the grain pest mite Aleuroglyphus ovatus (Acari: Acaridae). Exp Appl Acarol 91, 615–628 (2023). https://doi.org/10.1007/s10493-023-00861-9
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DOI: https://doi.org/10.1007/s10493-023-00861-9