Abstract
Both circular RNA eukaryotic translation initiation factor 6 (circEIF6) and microRNA (miR)-138-5p participate in thyroid cancer (TC) progression. Nevertheless, the relationship between them remains under-explored. Hence, this research ascertained the mechanism of circEIF6 in TC via miR-138-5p. After TC tissues and cells were harvested, circEIF6, miR-138-5p, and lipase H (LIPH) levels were assessed. The binding relationships among circEIF6, miR-138-5p, and LIPH were analyzed. The impacts of circEIF6, miR-138-5p, and LIPH on the invasive and proliferative abilities of TPC-1 cells were examined by Transwell and EdU assays. Tumor xenograft in nude mice was established for in vivo validation of the impact of circEIF6. CircEIF6 expression was high in TC cells and tissues. Additionally, miR-138-5p was poor and LIPH level was high in TC tissues. Mechanistically, circEIF6 competitively bound to miR-138-5p to elevate LIPH via a competitive endogenous RNA mechanism. Silencing of circEIF6 reduced TPC-1 cell proliferative and invasive properties, which was annulled by further inhibiting miR-138-5p or overexpressing LIPH. Likewise, circEIF6 silencing repressed the growth of transplanted tumors, augmented miR-138-5p expression, and diminished LIPH expression in nude mice. Conclusively, circEIF6 silencing reduced LIPH level by competitive binding to miR-138-5p, thus subduing the proliferation and invasion of TPC-1 cells.
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The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.
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Thanks for the grant from the Clinical Medical Technology Innovation Guidance Project (Grant No. 2020SK50906).
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YD and HJ conceived the ideas. YD, ZDX, ZZH, and ZS designed the experiments. YD, SBP, HCK, and LM performed the experiments. YD, ZDX, ZZH, and ZS analyzed the data. YD and HJ provided critical materials. YD, ZDX, and ZZH wrote the manuscript. HJ supervised the study. All the authors have read and approved the final version for publication.
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The experiments involving humans were ratified by the medical ethics committee of Hunan Provincial People’s Hospital (approval number: 2020120) and conformed to the Declaration of Helsinki. All operations in animals were performed after discussion and approval of the animal ethics committee in Hunan Provincial People’s Hospital (approval number: 2020120).
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Yi, D., Zhang, D., Zeng, Z. et al. Circular RNA eukaryotic translation initiation factor 6 facilitates TPC-1 cell proliferation and invasion through the microRNA-138-5p/lipase H axis. Funct Integr Genomics 23, 313 (2023). https://doi.org/10.1007/s10142-023-01240-8
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DOI: https://doi.org/10.1007/s10142-023-01240-8