Abstract
In this study, an analytical method for the determination of alcohols by gas chromatography-flame ionization detection was applied to fermented foods. The limit of detection and limit of quantitation were 0.79 and 2.40 mg/kg for methanol, 0.55 and 1.66 mg/kg for ethanol, 0.51 and 1.56 mg/kg for n-propanol, 0.35 and 1.05 mg/kg for n-butanol, and 0.38 and 1.16 mg/kg for n-pentanol, respectively. The recoveries from the matrices of gochujang, soy sauce, and kimchi were 93.80–102.03%, 93.27–99.69% and 89.06–102.17%, respectively, and the corresponding intra- and inter-day relative standard deviations were below 5.33%, 5.35% and 4.10%. In 95 fermented foods, ethanol showed the highest mean, median and maximum values among the five alcohols. The detection rate of ethanol was 86.3% among all samples and 100% in gochujang and gochujang-based sauces. A total of 22 samples had an alcohol content above 0.5%, of which 16 were gochujang.
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References
AOAC International. AOAC Official method 983.13. Official method of analysis. Gaithersburg (1988a)
AOAC International. AOAC Official method 984.14. Official method of analysis. Gaithersburg (1988b)
Alzeer J, Abou Hadeed K. Ethanol and its Halal status in food industries. Trends in Food Science & Technology. 58: 14-20 (2016)
Choo J-J, Shin H-J. Sensory evaluation and changes in physiochemical properties, and microflora and enzyme acitivities of pumpkin - added kochujang. Food Science and Biotechnoloigy. 32: 851-859 (2000)
Ding X, Wu C, Huang J, Zhou R. Characterization of interphase volatile compounds in Chinese Luzhou-flavor liquor fermentation cellar analyzed by head space-solid phase micro extraction coupled with gas chromatography mass spectrometry (HS-SPME/GC/MS). LWT-Food Science and Technology. 66: 124-133 (2016)
Food and Drug Administration. Guidelines for the validation of chemical methods in food, feed, cosmetics, and veterinary products. Food and Drug Administration: Silver Spring, MD, USA: 1-39 (2019)
Gil N-Y, Kim SY, Choi HS, Park SY, Kim JH. Investigation of quality characteristics and alcohol content in commercial Korean fermented sources. The Korean Society of Food Preservation. 23: 341-346 (2016)
Gunduz S, Yilmaz H, Goren AC. Halal food and metrology: Ethyl alcohol contents of beverages. Journal of Chemical Metrology. 7: 7-9 (2013)
Jamaludin MA, Hashim D, Rahman RA, Ramli MA, Majid MZA, Othman R, Amin A. Determination of permissible alcohol and vinegar in Shariah and scientific perspectives. International Food Research Journal. 23 (2016)
Mat Hashim D, Che Man Y, Sazili A. 3rd IMT-GT International Symposium on Halal Science and Management, pp. 21-22 (2009).
Najiha AA, Tajul A, Norziah M, Wan Nadiah W. A preliminary study on halal limits for ethanol content in food products. Middle-East Journal of Scientific Research. 6: 45-50 (2010)
Ordukaya E, Karlik B. Fruit juice–alcohol mixture analysis using machine learning and electronic nose. IEEJ Transactions on Electrical and Electronic Engineering. 11: S171-S176 (2016)
Park S, Kim J-C, Lee HS, Jeong S-W, Shim Y-S. Determination of five alcohol compounds in fermented Korean foods via simple liquid extraction with dimethyl-sulfoxide followed by gas chromatography-mass spectrometry for Halal food certification. LWT. 74: 563-570 (2016)
Park SW, Lee SJ, Sim YS, Choi JY, Park EY, Noh BS. Analysis of ethanol in soy sauce using electronic nose for halal food certification. Food Science and Biotechnology. 26: 311-317 (2017)
Pauzi N, Man S, Nawawi MSAM, Abu-Hussin MF. Ethanol standard in halal dietary product among Southeast Asian halal governing bodies. Trends in Food Science & Technology 86: 375-380 (2019)
Regenstein JM, Chaudry MM, Regenstein CE. The kosher and halal food laws. Comprehensive Reviews in Food Science and Food Safety. 2: 111-127 (2003)
Wan Nadiah W, Anis Najiha A, Tajul A, Norziah M. Determination of Halal limits for alcohol content in foods by simulated fermentation. IMT-GT. 21: 61 (2009)
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This work was supported by the Korea Food Research Institute [project number E0156200-01].
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Kim, Y., Shim, YS. & Lee, KG. Determination of alcohols in various fermented food matrices using gas chromatography-flame ionization detector for halal certification. Food Sci Biotechnol 31, 1639–1646 (2022). https://doi.org/10.1007/s10068-022-01156-2
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DOI: https://doi.org/10.1007/s10068-022-01156-2