Abstract
The aim of this study was to develop an efficient quantitative method for the determination of acetaldehyde (AA) and formaldehyde (FA) contents in solid and liquid food matrices. The determination of those compounds was validated and performed using gas chromatography-mass spectrometry combined by solid phase micro-extraction after derivatization with O-(2,3,4,5,6-pentafluoro-benzyl)-hydroxylamine hydrochloride. Validation was carried out in terms of limit of detection, limit of quantitation, linearity, precision, and recovery. Then their contents were analyzed in various food samples including 15 fruits, 22 milk products, 31 alcohol-free beverages, and 13 alcoholic beverages. The highest contents of AA and FA were determined in a white wine (40,607.02 ng/g) and an instant coffee (1,522.46 ng/g), respectively.
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References
International agency for research on cancer (IARC). (1985) Allyl compounds, aldehydes, epoxides and peroxides. IARC Monogr Eval Carcinog Risks Chem to Hum, 36, 101–132.
Miyake, T. and Shibamoto, T. (1993) Quantitative analysis of acetaldehyde in foods and beverages. J Agric. Food Chem., 41, 1968–1970.
Liu, S.Q. and Pilone, G.J. (2000) An overview of formation and roles of acetaldehyde in winemaking with emphasis on microbiological implications. Int. J. Food Sci. Technol., 35, 49–61.
Henschke, P.A. and Jiranek, V. (1993) Yeasts-metabolism of nitrogen compounds in Wine Microbiology and Biotechnology (Fleet, G.H. Ed.). Harwood Academic Publishers, Amsterdam, Germany, pp. 77–164.
Anli, R.E., Vural, N. and Gucer, Y. (2007) Determination of the principal volatile compounds of Turkish Raki. J. Inst. Brew., 113, 302–309.
Schönberg, A. and Moubacher, R. (1952) The Strecker degradation of α-amino acids. Chem. Rev., 50, 261–277.
Saijō, R. and Takeo, T. (1970) The formation of aldehydes from amino acids by tea leaves extracts. Agric. Biol. Chem., 34, 227–233.
Hsieh, R.J. and Kinsella, J.E. (1989) Lipoxygenase generation of specific volatile flavor carbonyl compounds in fish tissues. J. Agric. Food Chem., 37, 279–286.
Griffith, R. and Hammond, E.G. (1989) Generation of Swiss cheese flavor components by the reaction of amino acids with carbonyl compounds. J. Dairy Sci., 72, 604–613.
Hug, K., Lee, T.K., Park, J.M. and Shin, U.S. (1989) Preventive effect of ginseng butanol fraction against acetaldehydeinduced acute toxicity. Korean J. Ginseng Sci., 13, 5–7.
International agency for research on cancer (IARC). (2012) Consumption of alcoholic beverages: in personal habits and indoor combustions. IARC Monogr Eval Carcinog Risks to Hum, 100E, 373–499.
Norliana, S., Abdulamir, A.S., Abu Bakar, F. and Salech, A.B. (2009) The health risk of formaldehyde to human beings. Am. J. Parmacol. Toxicol., 4, 98–106.
Bianchi, F., Careri, M., Musci, M. and Mangia, A. (2007) Fish and food safety: determination of formaldehyde in 12 fish species by SPME extraction and GC-MS analysis. Food Chem., 100, 1049–1053.
Eur Food Safty Authority (EFSA). (2014) Endogenous formaldehyde turnover in humans compared with exogenous contribution from food sources. EFSA J., 12, 3550.
Velšek, J., Davidek, T., Davídek, J., Viden, I. and Trška, P. (1989) Some formaldehyde reaction products in non-enzymatic browning reactions. Z. Lebensm. Unters. Forsch., 188, 426–429.
Baan, R., Grosse, Y., Straif, K., Secretan, B., El Ghissassi, F., Bouvard, V., Benbrahim-Tallaa, L., Guha, N., Freeman, C., Galichet, L. and Cogliano, V. (2009) A review of human carcinogens-Part F: chemical agents and related occupations. Lancet Oncol., 10, 1143–1144.
International agency for research on cancer (IARC). (2006) Formaldehyde, 2-butaoxyethanol and 1-tert-butoxypropan-2-ol. IARC Monogr Eval Carcinog Risks to Hum, 88, 39–325.
National Toxicology Program (NTP). (2013) Department of health and human services, Public Health Service, Formaldehyde. NTP, 13, 1–11.
Wang, Q., O’Reilly, J. and Pawliszyn, J. (2005) Determination of low-molecular mass aldehydes by automated headspace solid-phase microextraction with in-fibre derivatization. J. Chromatogr. A, 1071, 147–154.
Satio, T., Takashina, T., Yanagisawa, S. and Shirai, T. (1983) Determination of trace low molecular weight aliphatic carbonyl compounds in auto exhaust by gas chromatography with a glass capillary column. Bunseki Kagaku, 32, 33–38.
Kuwata, K., Uebori, M., Yamasaki, H., Kuge, Y. and Kiso, Y. (1983) Determination of aliphatic aldehydes in air by liquid chromatography. Anal. Chem., 55, 2013–2016.
Sugaya, N., Nakagawa, T., Sakurai, K., Morita, M. and Onodera, S. (2001) Analysis of aldehydes in water by head space-GC/MS. J. Health Sci., 47, 21–27.
Park, Y.S., Lee, Y.J. and Lee, K.T. (2006) Analysis of formaldehyde and acetaldehyde in alcoholic beverage. J. Korean Soc. Food Sci. Nutr., 35, 1412–1419.
Hayashi, T., Reece, C.A. and Shibamoto, T. (1986) Gas chromatographic determination of formaldehyde in coffee via thazolidine derivative. J. Assoc. Off. Anal. Chem., 69, 101–105.
Uebelacker, M. and Lachenmeier, D.W. (2011) Quantitative determination of acetaldehyde in foods using automated digestion with simulated gastric fluid followed by headspace gas chromatography. J. Autom. Methods Manage. Chem., 2011, 907317.
Kaseleht, K., Paalme, T. and Nisamedtinov, I. (2011) Quantitative analysis of acetaldehyde in foods consumed by children using SPME/GC-MS(Tof), on-fiber derivatization and deuterated acetaldehyde as an internal standard. Agron. Res., 9, 395–341.
Paiano, V., Bianchi, G., Davoli, E., Negri, E., Fanelli, R. and Fattore, E. (2014) Risk assessment for the Italian population of acetaldehyde in alcoholic and non-alcoholic beverages. Food Chem., 154, 26–31.
European Commision. (1989) Council regulation No 1576/89 laying down general rules on the definition, description and presentation of spirit drinks. Off. J. Eur. Communities, L160, 1–17.
Shin, H.S. and Lim, H.H. (2012) Simple determination of formaldehyde in fermented foods by HS-SPME-GC/MS. Int. J. Food Sci. Technol., 47, 350–356.
Chung, H., Yoon, M.K., Han, J. and Kim, Y.S. (2015) Evaluation of volatile organic compounds in alcoholic beverages consumed in Korea. J. Korean Soc. Appl. Biol. Chem., 58, 423–432.
Arthur YAU. (2007) Formaldehyde in Food. Risk Communication Section, Centre for Food Safety. Available from: https://doi.org/www.cfs.gov.hk/.
Davis, P.L., Roe, B. and Bruemmer, J.H. (1973) Biochemical changes in citrus fruits during controlled atmosphere storage. J. Food Sci., 38, 225–229.
WHO. (2002) Concise International Chemical Assessment Document Series Formaldehyde. WHO, 40.
O’Brien, J., Nursten, H.E., Crabbe, M.J. and Ames, J.M. (1998) The maillard reaction in foods and medicine. Royal Society of Chemistry, Cambridge, pp. 57–64.
Sowiński, P., Wardenki, W., Partyka, M. (2005) Development and evaluation of headspace gas chromatography method for the analysis of carbonyl compounds in spirits and vodkas. Anal. Chim. Acta, 539, 17–22.
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Jeong, HS., Chung, H., Song, SH. et al. Validation and Determination of the Contents of Acetaldehyde and Formaldehyde in Foods. Toxicol Res. 31, 273–278 (2015). https://doi.org/10.5487/TR.2015.31.3.273
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DOI: https://doi.org/10.5487/TR.2015.31.3.273