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Zein film as a novel natural biopolymer membrane in electrochemical detections

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Abstract

Permselective modifier films are very important in preparing highly sensitive electrochemical sensors. In this work, for the first time, the behavior of gold and glassy carbon electrodes coated with biocompatible zein film as a permselective membrane for the electrochemical detection of various compounds has been investigated. For this purpose, several electroactive cationic (methylene blue, brilliant green, and thionine) and anionic (potassium ferricyanide, alizarin red S, and riboflavin-5’-phosphate) compounds have been used as model. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) showed that zein membranes prepared from casting solution containing 1% zein in ethanol/water have porous structures with high nanometric roughness. The capacitance values of electrical double layers of electrodes modified with zein film were very high for hydrophilic ions in comparison with hydrophobic ions. Point of zero charge pH (pHpzc) of zein membrane was 4.8. The results of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) as well as pHpzc study indicated that zein permselective membrane acts as ion exchanger film for selected cationic compounds with fast electrochemical kinetics responses in aqueous solution (pH=7). This behavior was confirmed by circulating solutions containing model compounds from homemade continuous cell equipped with polyamide membranes modified with zein film.

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References

  1. Brunetti B, Desimoni E (2014) Permselectivity and preconcentration properties of taurine/graphite oxide electrode coatings: Analytical perspectives. Electrochem Commun 43:51–54

    Article  CAS  Google Scholar 

  2. Perumal P, Christopher Selvin P (2020) Red algae-derived k-carrageenan-based proton-conducting electrolytes for the wearable electrical devices. J Solid State Electrochem 24(10):2249–2260

    Article  CAS  Google Scholar 

  3. Kiruthika S, Malathi M, Selvasekarapandian S, Tamilarasan K, Moniha V, Manjuladevi R (2019) Eco-friendly biopolymer electrolyte, pectin with magnesium nitrate salt, for application in electrochemical devices. J Solid State Electrochem 23(7):2181–2193

    Article  CAS  Google Scholar 

  4. Szentirmay MN, Martin C-R (1984) Ion-exchange selectivity of nafion films on electrode surfaces. Anal Chem 56(11):1898–1902

    Article  CAS  Google Scholar 

  5. Singh K, Shahi V-K (1998) Electrochemical studies on Nafion membrane. J Membr Sci 140(1):51–56

    Article  CAS  Google Scholar 

  6. Ciriello R, Cataldi T-R-I, Centonze D, Guerrieri A (2000) Permselective behavior of an electrosynthesized, nonconducting thin film of poly(2-naphthol) and its application to enzyme immobilization. Electroanal 12(11):825–830

    Article  CAS  Google Scholar 

  7. Ghita M, Arrigan D-W-M (2004) Electrochemical overoxidation of polyindole and its cation-permselective behavior. Electroanal 16(12):979–987

    Article  CAS  Google Scholar 

  8. Ozden M, Ekinci E, Karagozler A-E (1998) Synthesis and optimization of permselective polymer (polyindoline) film. J Solid State Electrochem 2:427–431

    Article  CAS  Google Scholar 

  9. Zhou L, Shang F, Pravda M, Glennon J-D, Luong J-H-T (2009) Selective detection of dopamine using glassy carbon electrode modified by a combined electropolymerized permselective film of polytyramine and polypyrrole-1-propionic acid. Electroanal 21:797–803

    CAS  Google Scholar 

  10. Michalska A, Gałuszkiewicz A, Ogonowska M, Ocypa M, Maksymiuk K (2004) PEDOT films: multifunctional membranes for electrochemical ion sensing. J Solid State Eletrochem 8(6):381–389

    Article  CAS  Google Scholar 

  11. Yano J, Shimoyama A, Ogura K (1992) Poly(o-phenylenediamine)-film-coated electrode having a permselective response to halogenide ions. J Electrochem Soc 139(5):L52–L53

    Article  CAS  Google Scholar 

  12. Guerrieri A, Ciriello R, Centonze D (2009) Permselective and enzyme-entrapping behaviours of an electropolymerized, non-conducting, poly(o-aminophenol) thin film-modified electrode: a critical study. Biosens Bioelectron 24(6):1550–1556

    Article  CAS  Google Scholar 

  13. Fattakhova-Rohlfing D, Wark M, Rathousky J (2007) Ion-permselective pH-switchable mesoporous silica thin layers. Chem Mater 19(7):1640–1647

    Article  CAS  Google Scholar 

  14. Elbert J, Krohm F, Rüttiger C, Kienle S, Didzoleit H, Balzer B-N, Hugel T, Stühn B, Gallei M, Brunsen A (2014) Polymer-modified mesoporous silica thin films for redox-mediated selective membrane gating. Adv Funct Mater 24(11):1591–1601

    Article  CAS  Google Scholar 

  15. Brunsen A, Micoureau S, Tagliazucchi M, Szleifer I, Azzaroni O, Soler-Illia G-J-A-A (2015) Mesoporous hybrid thin film membranes with PMETAC@silica architectures: controlling ionic gating through the tuning of polyelectrolyte density. Chem Mater 27(3):808–821

    Article  Google Scholar 

  16. Li S, Luo Q, Zhang Z, Shen G, Wu H, Chen A, Liu X, Li M, Zhang A (2018) Electrochemistry study of permselectivity and interfacial electron transfers of a branch-tailed fluorosurfactant self-assembled monolayer on gold. Molecules 23(11):2998–3012

    Article  Google Scholar 

  17. Özden M, Ekinci E, Karagözler E (1999) Electrochemical preparation of poly(o-toluidine) film and its application as a permselective membrane. J Appl Polym Sci 71(13):2141–2146

    Article  Google Scholar 

  18. Dong F, Padua G-W, Wang Y (2013) Controlled formation of hydrophobic surfaces by self-assembly of an amphiphilic natural protein from aqueous solutions. Soft Matter 9(25):5933–5941

    Article  CAS  Google Scholar 

  19. Pereira T-S, Oliveira G-C-M, Santos F-A, Raymundo-Pereirab P-A, Oliveira O-N, Janegitz B-C (2019) Use of zein microspheres to anchor carbon black and hemoglobin in electrochemical biosensors to detect hydrogen peroxide in cosmetic products, food and biological fluids. Talanta 194:737–744

    Article  Google Scholar 

  20. Chen X, Li D, Li G, Luo L, Ullah N, Wei Q, Huang F (2015) Facile fabrication of gold nanoparticle on zein ultrafine fibers and their application for catechol biosensor. Appl Surf Sci 328:444–452

    Article  CAS  Google Scholar 

  21. Hajilari F, Farhadi K, Eskandari H (2019) Extraction and trace analysis of trihalomethanes in water samples using zein@Fe3O4 nanocomposite. Bull Environ Contam Toxicol 102(4):581–588

    Article  CAS  Google Scholar 

  22. Azizi B, Farhadi K, Samadi N (2019) Functionalized carbon dots from zein biopolymer as a sensitive and selective fluorescent probe for determination of sumatriptan. Microchem J 146:965–973

    Article  CAS  Google Scholar 

  23. Jangju A, Farhadi K, Mehdi Hatami M, Amani S, Esma-ali F, Moshkabadi A, Hajilari F (2017) Application of zein-modified magnetite nanoparticles in dispersive magnetic micro-solid-phase extraction of synthetic food dyes in foodstuffs. J Sep Sci 40(6):1343–1352

    Article  CAS  Google Scholar 

  24. Cheng Q, Brajter-Toth A (1995) Permselectivity and high sensitivity at ultrathin monolayers. Effect of film hydrophobicity. Anal Chem 67(17):2767–2775

    Article  CAS  Google Scholar 

  25. Sun T, Feng L, Gao X, Jiang L (2005) Bioinspired surfaces with special wettability. Acc Chem Res 38(8):644–652

    Article  CAS  Google Scholar 

  26. Huddleston J-G, Visser A-E, Reichert W-M, Willauer H-D, Broker G-A, Rogers R-D (2001) Characterization and comparison of hydrophilic and hydrophobic room temperature ionic liquids incorporating the imidazolium cation. Green Chem 3(4):156–164

    Article  CAS  Google Scholar 

  27. Valente N-I, Muteto P-V, Farinha A-S, Tomé A-C, Oliveira J-A, Gomes M-T-S (2011) An acoustic wave sensor for the hydrophilic fluoride. Sensors Actuators B Chem 157(2):594–599

    Article  CAS  Google Scholar 

  28. Azzaroni O, Moya S, Farhan T, Brown A-A, Huck W-T-S (2005) Switching the properties of polyelectrolyte brushes via “Hydrophobic Collapse”. Macromolecules 38(24):10192–10199

    Article  CAS  Google Scholar 

  29. Hu K, Xub B, Shao H-B (2015) Determination of hydrophobicity scale of tetraphenylborate and its derivatives by ferrocene based three-phase electrodes. Electrochem Commun 50:36–38

    Article  CAS  Google Scholar 

  30. Wang Q, Wang J-F, Geil P-H, Padua G-W (2004) Zein adsorption to hydrophilic and hydrophobic surfaces investigated by surface plasmon resonance. Biomacromolecules 5(4):1356–1361

    Article  CAS  Google Scholar 

  31. Wang Y, Padua G-W (2004) Water sorption properties of extruded zein films. J Agric Food Chem 52(10):3100–3105

    Article  CAS  Google Scholar 

  32. Porter M-D, Bright T-B, Allara D-L, Chidsey C-E-D (1987) Spontaneously organized molecular assemblies. 4. Structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared Spectroscopy, and electrochemistry. J Am Chem Soc 109(12):3559–3568

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, Urmia University, Urmia, Iran

    Mahsa Hasanzadeh & Khalil Farhadi

  2. Anistitue of Nanotechnology, Urmia University, Urmia, Iran

    Khalil Farhadi

  3. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

    Hossein Tajik

Authors
  1. Mahsa Hasanzadeh
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  2. Khalil Farhadi
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  3. Hossein Tajik
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Correspondence to Khalil Farhadi.

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Hasanzadeh, M., Farhadi, K. & Tajik, H. Zein film as a novel natural biopolymer membrane in electrochemical detections. J Solid State Electrochem 25, 1327–1337 (2021). https://doi.org/10.1007/s10008-021-04910-4

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  • DOI: https://doi.org/10.1007/s10008-021-04910-4

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