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Preventive effect of chitosan gel containing CaneCPI-5 against enamel erosive wear in situ

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Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objective

This study evaluated the preventive effect of a chitosan gel containing CaneCPI-5 against enamel erosion and erosion + abrasion in situ.

Methods

Sixteen volunteers participated in a crossover, double-blind protocol, comprising 4 phases: (1) no treatment (Nt); (2) chitosan gel (Cg); (3) chitosan gel + 12,300 ppm NaF (Cg + NaF); and (4) chitosan gel + 0.1 mg/mL CaneCPI-5 (Cg + Cane). Volunteers wore an appliance containing 4 specimens. Once/day, they applied the gel (except for Nt) (4 min/specimen). Erosive challenges were performed extra-orally (0.1% citric acid, 90 s, 4 × /day; ERO). Specimens were also abraded (toothbrush, 15 s/specimen, 2 × /day; ERO + ABR). Enamel wear was assessed by profilometry and relative surface reflection intensity (%SRI). Two-way RM-ANOVA/Sidak’s tests and Spearman’s correlation were used (p < 0.05).

Results

For profilometry, ERO + ABR promoted significantly greater wear when compared with ERO. There was a significant difference among all treatments. The lowest enamel loss occurred for Cg + Cane, followed by Cg + NaF, Cg, and Nt (p < 0.05). The %SRI was significantly lower for ERO + ABR when compared to ERO, only for the Nt group. The greatest %SRI was found for the Cg + NaF and Cg + Cane groups, which did not differ significantly, regardless of the conditions. The lowest %SRI was found for the Nt and Cg groups, which did not differ from each other, regardless of the conditions. The Nt group did not differ significantly from the Cg + NaF (ERO). There was a significant correlation between both analyses.

Conclusion

The incorporation of CaneCPI-5 in the chitosan gel prevented erosive wear in situ.

Clinical relevance

These results open a new perspective for the use of CaneCPI-5 in other application vehicles, such as chitosan gel.

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References

  1. Schlueter N, Amaechi BT, Bartlett D, Buzalaf MAR, Carvalho TS, Ganss C, Hara AT, Huysmans M, Lussi A, Moazzez R, Vieira AR, West NX, Wiegand A, Young A, Lippert F (2020) Terminology of erosive tooth wear: consensus report of a workshop organized by the ORCA and the Cariology Research Group of the IADR. Caries Res 54:2–6. https://doi.org/10.1159/000503308

    Article  PubMed  Google Scholar 

  2. Buzalaf MAR, Magalhaes AC, Rios D (2018) Prevention of erosive tooth wear: targeting nutritional and patient-related risks factors. Br Dent J 224:371–378. https://doi.org/10.1038/sj.bdj.2018.173

    Article  PubMed  Google Scholar 

  3. Voronets J, Lussi A (2010) Thickness of softened human enamel removed by toothbrush abrasion: an in vitro study. Clin Oral Investig 14:251–256. https://doi.org/10.1007/s00784-009-0288-y

    Article  PubMed  Google Scholar 

  4. Lussi A, Schlueter N, Rakhmatullina E, Ganss C (2011) Dental erosion–an overview with emphasis on chemical and histopathological aspects. Caries Res 45:2–12. https://doi.org/10.1159/000325915

    Article  PubMed  Google Scholar 

  5. Bartlett DW, Lussi A, West NX, Bouchard P, Sanz M, Bourgeois D (2013) Prevalence of tooth wear on buccal and lingual surfaces and possible risk factors in young European adults. J Dent 41:1007–1013. https://doi.org/10.1016/j.jdent.2013.08.018

    Article  PubMed  Google Scholar 

  6. Salas MMS, Nascimento GG, Huysmans MC, Demarco FF (2015) Estimated prevalence of erosive tooth wear in permanent teeth of children and adolescents: an epidemiological systematic review and meta-regression analysis. J Dent 43:42–50. https://doi.org/10.1016/j.jdent.2014.10.012

    Article  PubMed  Google Scholar 

  7. Vieira Pedrosa BR, de Menezes VA (2020) Prevalence of erosive tooth wear and related risk factors in adolescents: an integrative review. J Dent Child 87:18–25

    Google Scholar 

  8. Bezerra SJC, Viana IEL, Aoki IV, Sobral MAP, Borges AB, Hara AT, Scaramucci T (2021) Erosive tooth wear inhibition by hybrid coatings with encapsulated fluoride and stannous ions. J Mater Sci Mater Med 32:83. https://doi.org/10.1007/s10856-021-06554-2

    Article  PubMed  PubMed Central  Google Scholar 

  9. Rios D, Boteon AP, Di Leone CCL, Castelluccio TT, Mendonca FL, Ionta FQ, Buzalaf MAR, Carvalho TS (2021) Vitamin E: a potential preventive approach against dental erosion-an in vitro short-term erosive study. J Dent 113:103781. https://doi.org/10.1016/j.jdent.2021.103781

    Article  PubMed  Google Scholar 

  10. Vivek HP, Prashant GM, Geetha S, Chandramohan S, Imranulla M, Srinidhi PB (2018) effect of mouthrinses containing olive oil, fluoride, and their combination on enamel erosion: an in vitro study. J Contemp Dent Pract 19:130–136

    Article  PubMed  Google Scholar 

  11. Niemeyer SH, Baumann T, Lussi A, Meyer-Lueckel H, Scaramucci T, Carvalho TS (2021) Salivary pellicle modification with polyphenol-rich teas and natural extracts to improve protection against dental erosion. J Dent 105:103567. https://doi.org/10.1016/j.jdent.2020.103567

    Article  PubMed  Google Scholar 

  12. Santiago AC, Khan ZN, Miguel MC, Gironda CC, Soares-Costa A, Pela VT, Leite AL, Edwardson JM, Buzalaf MAR, Henrique-Silva F (2017) A new sugarcane cystatin strongly binds to dental enamel and reduces erosion. J Dent Res 96:1051–1057. https://doi.org/10.1177/0022034517712981

    Article  PubMed  Google Scholar 

  13. Carvalho TS, Araújo TT, Ventura TMO, Dionizio A, Câmara JVF, Moraes SM, Pelá VY, Martini T, Leme JC, Derbotolli ALB, Grizzo LT, Crusca E, Shibao PYT, Marchetto R, Henrique-Silva F, Pessan JP, Buzalaf MAR (2020) Acquired pellicle protein-based engineering protects against erosive demineralization. J Dent 102:103478. https://doi.org/10.1016/j.jdent.2020.103478

    Article  PubMed  Google Scholar 

  14. Pela VT, Buzalaf MAR, Niemeyer SH, Baumann T, Henrique-Silva F, Toyama D, Crusca E, Marchetto R, Lussi A, Carvalho TS (2021) Acquired pellicle engineering with proteins/peptides: mechanism of action on native human enamel surface. J Dent 107:103612. https://doi.org/10.1016/j.jdent.2021.103612

    Article  PubMed  Google Scholar 

  15. Pela VT, Lunardelli JGQ, Tokuhara CK, Gironda CC, Silva NDG, Carvalho TS, Santiago AC, Souza BM, Moraes SM, Henrique-Silva F, Magalhaes AC, Oliveira RC, Buzalaf MAR (2021) Safety and in situ antierosive effect of CaneCPI-5 on dental enamel. J Dent Res 100:1344–1350. https://doi.org/10.1177/00220345211011590

    Article  PubMed  Google Scholar 

  16. Santos LA, Martini T, Camara JVF, Reis FN, Ortiz AC, Camiloti GD, Levy FM, Shibao P, Honorio HM, Henrique-Silva F, Pieretti JC, Seabra AB, Cardoso CAB, Buzalaf MAR (2021) Solutions and gels containing a sugarcane-derived cystatin (CaneCPI-5) reduce enamel and dentin erosion in vitro. Caries Res 55:594–602. https://doi.org/10.1159/000520261

    Article  PubMed  Google Scholar 

  17. Buzalaf MAR, Hannas AR, Kato MT (2012) Saliva and dental erosion. J Appl Oral Sci 20:493–502. https://doi.org/10.1590/S1678-77572012000500001

    Article  PubMed  PubMed Central  Google Scholar 

  18. Creeth JE, Burnett GR, Souverain A, Gomez-Pereira P, Zero DT, Lippert F, Hara AT (2020) In situ efficacy of an experimental toothpaste on enamel rehardening and prevention of demineralisation: a randomised, controlled trial. BMC Oral Health 20:118. https://doi.org/10.1186/s12903-020-01081-y

    Article  PubMed  PubMed Central  Google Scholar 

  19. Zanatta RF, Caneppele TMF, Scaramucci T, El Dib R, Maia LC, Ferreira D, Borges AB (2020) Protective effect of fluorides on erosion and erosion/abrasion in enamel: a systematic review and meta-analysis of randomized in situ trials. Arch Oral Biol 120:104945. https://doi.org/10.1016/j.archoralbio.2020.104945

    Article  PubMed  Google Scholar 

  20. Lippert F (2013) An introduction to toothpaste - its purpose, history and ingredients. Monogr Oral Sci 23:1–14. https://doi.org/10.1159/000350456

    Article  PubMed  Google Scholar 

  21. Kato MT, Leite AL, Hannas AR, Buzalaf MA (2010) Gels containing MMP inhibitors prevent dental erosion in situ. J Dent Res 89:468–472. https://doi.org/10.1177/0022034510363248

    Article  PubMed  Google Scholar 

  22. Ganss C, von Hinckeldey J, Tolle A, Schulze K, Klimek J, Schlueter N (2012) Efficacy of the stannous ion and a biopolymer in toothpastes on enamel erosion/abrasion. J Dent 40:1036–1043. https://doi.org/10.1016/j.jdent.2012.08.005

    Article  PubMed  Google Scholar 

  23. Schlueter N, Klimek J, Ganss C (2014) Effect of a chitosan additive to a Sn2+-containing toothpaste on its anti-erosive/anti-abrasive efficacy–a controlled randomised in situ trial. Clin Oral Investig 18:107–115. https://doi.org/10.1007/s00784-013-0941-3

    Article  PubMed  Google Scholar 

  24. Lee HS, Tsai S, Kuo CC, Bassani AW, Pepe-Mooney B, Miksa D, Masters J, Sullivan R, Composto RJ (2012) Chitosan adsorption on hydroxyapatite and its role in preventing acid erosion. J Colloid Interface Sci 385(1):235–243. https://doi.org/10.1016/j.jcis.2012.06.074

    Article  PubMed  Google Scholar 

  25. Bekale L, Agudelo D, Tajmir-Riahi HA (2015) Effect of polymer molecular weight on chitosan-protein interaction. J Colloid Interface Sci 125:309–317. https://doi.org/10.1016/j.colsurfb.2014.11.037

    Article  Google Scholar 

  26. de Souza BM, Santi LRP, de Souza SM, Buzalaf MAR, Magalhaes AC (2018) Effect of an experimental mouth rinse containing NaF and TiF4 on tooth erosion and abrasion in situ. J Dent 73:45–49. https://doi.org/10.1016/j.jdent.2018.04.001

    Article  PubMed  Google Scholar 

  27. Soares-Costa A, Beltramini LM, Thiemann OH, Henrique-Silva F (2002) A sugarcane cystatin: recombinant expression, purification, and antifungal activity. Biochem Biophys Res Commun 296:1194–1199. https://doi.org/10.1016/S0006-291X(02)02046-6

    Article  PubMed  Google Scholar 

  28. Carvalho TS, Baumann T, Lussi A (2016) A new hand-held optical reflectometer to measure enamel erosion: correlation with surface hardness and calcium release. Sci Rep 6:25259. https://doi.org/10.1038/srep25259

    Article  PubMed  PubMed Central  Google Scholar 

  29. Alencar CR, Mendonça FL, Guerrini LB, Jordão MC, Oliveira GC, Honório HM, Magalhães AC, Rios D (2016) Effect of different salivary exposure times on the rehardening of acid-softened enamel. Braz Oral Res 30:e104. https://doi.org/10.1590/1807-3107BOR-2016.vol30.0104

    Article  PubMed  Google Scholar 

  30. Laurance-Young P, Bozec L, Gracia L, Rees G, Lippert F, Lynch RMJ, Knowles JC (2011) A review of the structure of human and bovine dental hard tissues and their physicochemical behaviour in relation to erosive challenge and remineralisation. J Dent 39:266–272. https://doi.org/10.1016/j.jdent.2011.01.008

    Article  PubMed  Google Scholar 

  31. Pelá VT, Cassiano LPS, Ventura TMDS, Souza-E-Silva CM, Gironda CC, Rios D, Buzalaf MAR (2018) Proteomic analysis of the acquired enamel pellicle formed on human and bovine tooth: a study using the Bauru in situ pellicle model (BISPM). J Appl Oral Sci 27:20180113. https://doi.org/10.1590/1678-7757-2018-0113

    Article  Google Scholar 

  32. Pelá VT, Lunardelli JGQ, Ventura TMO, Camiloti GD, Baumann T, Carvalho TS, Lussi A, Buzalaf MAR (2020) Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo. Eur J Oral Sci 128:487–494. https://doi.org/10.1111/eos.12744

    Article  PubMed  Google Scholar 

  33. Hawkins R, Locker D, Noble J, Kay EJ (2003) Prevention. Part 7: professionally applied topical fluorides for caries prevention. Br Dent J 195:313–317. https://doi.org/10.1038/sj.bdj.4810527

    Article  PubMed  Google Scholar 

  34. Hannig M, Hannig C (2014) The pellicle and erosion. Monogr Oral Sci 25:206–214. https://doi.org/10.1159/000360376

    Article  PubMed  Google Scholar 

  35. Lussi A, Buzalaf MAR, Duangthip D, Anttonen V, Ganss C, Joao-Souza SG, Baumann T, Carvalho TS (2019) The use of fluoride for the prevention of dental erosion and erosive tooth wear in children and adolescents. Eur Arch Paediatr Dent 20:517–527. https://doi.org/10.1007/s40368-019-00420-0

    Article  PubMed  Google Scholar 

  36. Ten Cate JM, Buzalaf MAR (2019) Fluoride mode of action: once there was an observant dentist. J Dent Res 98:725–730. https://doi.org/10.1177/0022034519831604

    Article  PubMed  Google Scholar 

  37. Huysmans MC, Young A, Ganss C (2014) The role of fluoride in erosion therapy. Monogr Oral Sci 25:230–243. https://doi.org/10.1159/000360555

    Article  PubMed  Google Scholar 

  38. Litonjua LA, Andreana S, Bush PJ, Cohen RE (2003) Toothbrushing and gingival recession. Int Dent J 53:67–72. https://doi.org/10.1111/j.1875-595X.2003.tb00661.x

    Article  PubMed  Google Scholar 

  39. Borysenko A, Dudnikova M (2021) Clinical rationale of choosing a tooth-bleaching agent. Georgian Med News 313:48–51

    Google Scholar 

  40. Lipei C, Xiangke C, Xiaoyan O (2017) Brushing abrasion of the enamel surface after erosion. Hua Xi Kou Qiang Yi Xue Za Zhi 35:379–383. http://www.hxkqyxzz.net/CN/10.7518/hxkq.2017.04.007

  41. Carvalho TS, Baumann T, Lussi A (2016) Does erosion progress differently on teeth already presenting clinical signs of erosive tooth wear than on sound teeth? An in vitro pilot trial. BMC Oral Health 17:14. https://doi.org/10.1186/s12903-016-0231-y

    Article  PubMed  PubMed Central  Google Scholar 

  42. Lussi A, Bossen A, Höschele C, Beyeler B, Megert B, Meier C, Rakhmatullina E (2012) Effects of enamel abrasion, salivary pellicle, and measurement angle on the optical assessment of dental erosion. J Biomed Opt 17:97009. https://doi.org/10.1117/1.JBO.17.9.097009

    Article  PubMed  Google Scholar 

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Funding

The authors thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) for concession of scholarship provided to Vinícius Taioqui Pelá (2017/04857–4), Leonardo Brito (2021/02366–9), Joana Claudio Pieretti (2020/03646–2) and regular research grants to Marília Afonso Rabelo Buzalaf (2018/12041–7) and to Amedea Barozzi Seabra (2018/08194–2). Flávio Henrique-Silva and Amedea Barozzi Seabra are the recipients of Research Productivity Scholarship from the National Council for Research and Development (CNPq 311746/2017–9, CNPq 313117/2019–5, respectively). M.A.R.B is the recipient of a Research Productivity Scholarship from the National Council for Research and Development (CNPq 302371/2018–4). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil, under finance code 001.

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Authors and Affiliations

  1. Department of Genetics and Evolution, São Carlos Federal University, São Carlos, São Paulo, Brazil

    Vinícius Taioqui Pelá, Flávio Henrique-Silva & Eduardo Pereira de Souza

  2. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil

    Leonardo Brito, Even Akemi Taira & Marília Afonso Rabelo Buzalaf

  3. Center for Natural and Human Sciences, Federal University of ABC, Santo André, São Paulo, Brazil

    Joana Claudio Pieretti & Amedea Barozzi Seabra

  4. Postgraduate Department, Cruzeiro do Sul University, São Paulo, São Paulo, Brazil

    Cristiane de Almeida Baldini Cardoso

  5. Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Sonia Groisman

  6. Municipal University of São Caetano Do Sul (USCS), São Caetano do Sul, São Paulo, Brazil

    Marcela Charantola Rodrigues

  7. Department of Operative Dentistry and Periodontology, University Medical Centre, Freiburg, Germany

    Adrian Lussi

  8. Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland

    Thiago Saads Carvalho

Authors
  1. Vinícius Taioqui Pelá
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  2. Leonardo Brito
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  7. Cristiane de Almeida Baldini Cardoso
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  8. Eduardo Pereira de Souza
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  10. Marcela Charantola Rodrigues
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  11. Adrian Lussi
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  12. Thiago Saads Carvalho
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  13. Marília Afonso Rabelo Buzalaf
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Contributions

Conceptualization: Marília Afonso Rabelo Buzalaf; methodology: Vinícius Taioqui Pelá, Leonardo Brito, Even Akemi Taira, Joana Claudio Pieretti, Eduardo Pereira de Souza; formal analysis and investigation: Vinícius Taioqui Pelá, Flávio Henrique-Silva, Amedea Barozzi Seabra, Cristiane de Almeida Baldini Cardoso, Sonia Groisman, Marcela Charantola Rodrigues, Adrian Lussi, Thiago Saads Carvalho; writing—original draft preparation: Vinícius Taioqui Pelá and Marília Afonso Rabelo Buzalaf. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Marília Afonso Rabelo Buzalaf.

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Ethics approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This work was approved by the Research Ethics Committee of Bauru School of Dentistry, University of São Paulo, SP, Brazil (CAAE: 86,783,418.8.0000.5417). The use of bovine teeth for this research was also approved by the Ethics Committee on Animal Use of the Bauru School of Dentistry, University of São Paulo (Protocol: 005/2018).

Consent to participate

Informed consent was obtained from all individual participants included in the study. Sixteen volunteers (eight women and eight men) took part in the study, after signing an informed consent document. The volunteers participated in 4 crossover and double-blind phases.

Conflict of interest

The authors declare no competing interests.

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Pelá, V.T., Brito, L., Taira, E.A. et al. Preventive effect of chitosan gel containing CaneCPI-5 against enamel erosive wear in situ. Clin Oral Invest 26, 6511–6519 (2022). https://doi.org/10.1007/s00784-022-04600-z

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