Skip to main content

Advertisement

Log in

Thickness of softened human enamel removed by toothbrush abrasion: an in vitro study

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

The aim of the study was to assess the thickness of softened enamel removed by toothbrushing. Human enamel specimens were indented with a Knoop diamond. Softening was performed with citric acid or orange juice. The specimens were brushed in a brushing machine with a manual soft toothbrush in toothpaste slurry or in artificial saliva. Enamel loss was calculated from the change in indentation depth of the same indent before and after abrasion. Mean surface losses (95% confidence interval) were recorded in treatment groups (in nanometers): (1) citric acid, abrasion with slurry = 339 (280–398); (2) citric acid, abrasion with artificial saliva = 16 (5–27); (3) orange juice, abrasion with slurry = 268 (233–303); (4) orange juice, abrasion with artificial saliva = 14 (5–23); (5) no softening, abrasion with slurry = 28 (10–46). The calculated thickness of the softened enamel varied between 254 and 323 nm, depending on the acid used.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Ten Cate JM, Imfeld T (1996) Dental erosion, summary. Eur J Oral Sci 104:241–244

    Article  PubMed  Google Scholar 

  2. Featherstone JDB, Lussi A (2006) Understanding the chemistry of dental erosion. In: Lussi A (ed) Dental erosion. Karger, Basel, pp 66–76

    Chapter  Google Scholar 

  3. Ten Cate JM, Larsen MJ, Pearce EIF, Fejerskov O (2003) Chemical interactions between the tooth and oral fluids. In: Fejerskov O, Kidd E (eds) Dental caries: the disease and its clinical management. Blackwell Munksgaard, Oxford, pp 59–65

    Google Scholar 

  4. Dawes C (2003) What is the critical pH and why does a tooth dissolve in acid? J Can Dent Assoc 69:722–724

    PubMed  Google Scholar 

  5. Jaeggi T, Lussi A (1999) Toothbrush abrasion of erosively altered enamel after intraoral exposure to saliva: an in situ study. Caries Res 33:455–461

    Article  PubMed  Google Scholar 

  6. Wiegand A, Wegehaupt F, Werner C, Attin T (2007) Susceptibility of acid-softened enamel to mechanical wear-ultrasonication versus toothbrushing abrasion. Caries Res 41:56–60

    Article  PubMed  Google Scholar 

  7. Addy M (2005) Tooth brushing, tooth wear and dentine hypersensitivity—are they associated? Int Dent J 55:261–267

    PubMed  Google Scholar 

  8. Hemingway CA, Parker DM, Addy M, Barbour ME (2006) Erosion of enamel by non-carbonated soft drinks with and without toothbrushing abrasion. Br Dent J 201:447–450

    Article  PubMed  Google Scholar 

  9. Attin T (2006) Methods for assessment of dental erosion. In: Lussi A (ed) Dental erosion. Karger, Basel, pp 152–172

    Chapter  Google Scholar 

  10. Otis LL, Everett MJ, Sathyam US, Colston BW (2000) Optical coherence tomography: a new imaging technology for dentistry. J Am Dent Assoc 131:511–514

    PubMed  Google Scholar 

  11. Lussi A, Jaeggi T, Schaerer S (1993) The influence of different factors on in vitro enamel erosion. Caries Res 27:387–393

    Article  PubMed  Google Scholar 

  12. Zero DT, Rahbek I, Fu J, Proskin HM, Featherstone JDB (1990) Comparison of the iodide permeability test, the surface microhardeness test and mineral dissolution of bovine enamel following acid challenge. Caries Res 24:181–188

    Article  PubMed  Google Scholar 

  13. Chen PS, Toribara TY, Warner H (1956) Microdetermination of phosphorus. Anal Chem 28:1756–1758

    Article  Google Scholar 

  14. Lussi A, Hellwig E (2001) Erosive potential of oral care products. Caries Res 35:52–56

    Article  PubMed  Google Scholar 

  15. Newby CS, Creeth JE, Rees GD, Schemehorn BR (2006) Surface microhardness changes, enamel fluoride uptake, and fluoride availability from commercial toothpastes. J Clin Dent 17:94–99

    PubMed  Google Scholar 

  16. Brunner E, Domhof S, Langer F (2002) Nonparametric analysis of longitudinal data in factorial designs. Wiley, New York

    Google Scholar 

  17. British Standards Institution (1979) Precision of test methods. Guide for the determination and reproducibility for a standard test method (BS 5497, part 1). British Standards Institution, London

    Google Scholar 

  18. Bland JM, Altman DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1:307–310

    PubMed  Google Scholar 

  19. Larsen MJ, Nyvad B (1999) Enamel erosion by some soft drinks and orange juices relative to their pH, buffering effect and contents of calcium phosphate. Caries Res 33:81–87

    Article  PubMed  Google Scholar 

  20. Attin T, Buchalla W, Gollner M, Hellwig E (2000) Use of variable remineralization periods to improve the abrasion resistance of previously eroded enamel. Caries Res 34:48–52

    Article  PubMed  Google Scholar 

  21. Eisenburger M, Addy M (2003) Influence of liquid temperature and flow rate on enamel erosion and surface softening. J Oral Rehab 30:1076–1080

    Article  Google Scholar 

  22. Ganss C, Klimek J, Lussi A (2006) Accuracy and consistency of the visual diagnosis of exposed dentine on worn occlusal/incisal surfaces. Caries Res 40:208–212

    Article  PubMed  Google Scholar 

  23. Lippert F, Parker DM, Jandt KD (2004) Toothbrush abrasion of surface softened enamel studied with tapping mode AFM and AFM nanoindentation. Caries Res 38:464–472

    Article  PubMed  Google Scholar 

  24. Voronets J, Jaeggi T, Buergin W, Lussi A (2008) Controlled toothbrush abrasion of softened human enamel. Caries Res 42:286–290

    Article  PubMed  Google Scholar 

  25. Parry J, Harrington E, Rees GD, McNab R, Smith AJ (2008) Control of brushing variables for the in vitro assessment of toothpaste abrasivity using a novel laboratory model. J Dent 36:117–124

    Article  PubMed  Google Scholar 

  26. Joiner A, Schwarz A, Philpotts CJ, Cox TF, Huber K, Hannig M (2008) The protective nature of pellicle towards toothpaste abrasion on enamel and dentine. J Dent 36:360–368

    Article  PubMed  Google Scholar 

Download references

Acknowledgement

The authors wish to thank Stefanie Hayoz and Prof. J. Huesler for the statistical advice.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to J. Voronets.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Voronets, J., Lussi, A. Thickness of softened human enamel removed by toothbrush abrasion: an in vitro study. Clin Oral Invest 14, 251–256 (2010). https://doi.org/10.1007/s00784-009-0288-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-009-0288-y

Keywords

Navigation