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Oral bacterial decontamination using an innovative prototype for photocatalytic disinfection

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Abstract

Objectives

The aim of this study was to evaluate the effectiveness of a prototype photocatalytic device for bacterial decontaminations of the oral cavity.

Methods

Sixty-four subjects (18–65) were selected and randomly assigned to eight groups (n = 8), according to oral disinfection protocol: (G1): distilled water (control); (G2): 1.5% hydrogen peroxide (HP); (G3): 3.0% HP; (G4): 0.12% chlorhexidine (CHX); (G5): Germinator; (G6): 1.5% HP + Germinator; (G7): 3.0%HP + Germinator; (G8): 0.12% CHX + Germinator. Stimulated saliva was collected before and after a 3-min mouthwash and/or Germinator application. The patients were kept relaxed and retained saliva 5–10 min, spitting out into the tube for 3 min. The percentage bacterial reduction was checked by counting the colony-forming units (CFUs) after culturing on blood agar plates. Data were subjected to one-way ANOVA followed by Tukey’s post hoc test (α = 5%) for statistical significance.

Results

The highest bacterial reduction was observed in groups 3 (3.0% HP), 6 (1.5% HP + Germinator), and 7 (3.0% + Germinator), with no statistically significant difference between them (p > 0.05). Groups 6 (1.5% HP + Germinator) and 8 (0.12% CHX + Germinator) showed higher bacterial reduction than groups 2 (1.5% HP) and 4 (0.12% CHX) (p < 0.05). Finally, group 5 (Germinator) showed higher bacterial reduction than control group (DW) and group 4 (0.12% CHX) (p < 0.05).

Conclusions

The photocatalytic disinfection was effective against oral bacteria and improved the antimicrobial action of 1.5% HP and 0.12%.

Clinical significance.

The photocatalytic disinfection can be an alternative protocol to provide the oral decontamination.

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Authors and Affiliations

  1. School of Dentistry, University of Passo Fundo, Building A7, Suite 2, São JoséPasso Fundo, RS, 99052-900, Brazil

    Matheus Albino Souza, Daniela Jorge Corralo & Ezequiel Santin Gabrielli

  2. School of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil

    José Antônio Poli Figueiredo

  3. School of Dentistry, University of the Pacific, San Francisco, CA, USA

    Stephen Cohen

  4. School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Mark Wolff & Liviu Steier

  5. Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India

    Liviu Steier

Authors
  1. Matheus Albino Souza
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  2. Daniela Jorge Corralo
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  3. Ezequiel Santin Gabrielli
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  4. José Antônio Poli Figueiredo
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  5. Stephen Cohen
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  6. Mark Wolff
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  7. Liviu Steier
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Corresponding author

Correspondence to Matheus Albino Souza.

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Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed consent

Informed consent was obtained from all human participants included in the study.

Conflict of interest

Dr. Steier owns IP rights on the Germinator. The rest of the authors claim no conflicts of interest. The rest of the authors have no financial affiliation (e.g., employment, direct payment, stock holdings, retainers, consultantships, patent licensing arrangements, or honoraria) or involvement with any commercial organization with direct financial interest in the subject or materials discussed in this manuscript, nor have any such arrangements existed in the past 3 years. Any other potential conflict of interest is disclosed.

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Souza, M.A., Corralo, D.J., Gabrielli, E.S. et al. Oral bacterial decontamination using an innovative prototype for photocatalytic disinfection. Clin Oral Invest 26, 3005–3010 (2022). https://doi.org/10.1007/s00784-021-04282-z

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  • DOI: https://doi.org/10.1007/s00784-021-04282-z

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