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Serological methods for detection of infection with shrew-borne hantaviruses: Thottapalayam, Seewis, Altai, and Asama viruses

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Abstract

The infectivity of shrew-borne hantaviruses to humans is still unclear because of the lack of a serodiagnosis method for these viruses. In this study, we prepared recombinant nucleocapsid (rN) proteins of Seewis orthohantavirus, Altai orthohantavirus (ALTV), Thottapalayam thottimvirus (TPMV), and Asama orthohantavirus. Using monospecific rabbit sera, no antigenic cross-reactivity was observed. In a serosurvey of 104 samples from renal patients and 271 samples from heathy controls from Sri Lanka, one patient serum and two healthy control sera reacted with rN proteins of ALTV and TPMV, respectively. The novel assays should be applied to investigate potential infectivity of shrew-borne hantaviruses to humans.

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Funding

This work was partially supported by grants from the JSPS KAKENHI (19K10595) and by the Science and Technology Research Partnership for Sustainable Development (19jm0110019h0002) and by the Research Program on Emerging and Re-emerging Infectious Diseases, Japan Agency for Medical Research and Development (AMED) (JP18fk0108017 and JP20fk0108097). A grant provided by the National Science Foundation of Sri Lanka (RPHS/2016/CKDu/06) was received by Rohana Chandrajith (Study 03 PI—Chandika D. Gamage), Department of Geology, Faculty of Science, University of Peradeniya, Sri Lanka.

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Authors and Affiliations

  1. Graduate School of Infectious Diseases, Hokkaido University, Sapporo, 060-8638, Japan

    Zhouoxing Wei, Devinda S. Muthusinghe, Sithumini M. W. Lokupathirage & Kumiko Yoshimatsu

  2. Department of Microbiology and Immunology, Faculty of Medicine, Hokkaido University, Sapporo, 060-8638, Japan

    Kenta Shimizu, Kumpei Nishigami, Yoshimi Tsuda, Jiro Arikawa & Kumiko Yoshimatsu

  3. Graduate School of Medicine, Hokkaido University, Sapporo, Japan

    Kenta Shimizu, Yoshimi Tsuda & Jiro Arikawa

  4. Department of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka

    Yomani Sarathukumara & Chandika D. Gamage

  5. Girandrukotte District Hospital, Renal Clinic, District Hospital, Girandurukotte, Sri Lanka

    Lishanta Granathne

  6. Nephrology and Transplantation Unit, Teaching Hospital Kandy, Kandy, Sri Lanka

    Nishanta Nanayakkara

  7. National Institute of Infectious Diseases, Tokyo, Japan

    Fuka Kikuchi, Keiko Tanaka-Taya, Motoi Suzuki, Shigeru Morikawa & Satoru Arai

  8. Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, Fuchu, Japan

    Fuka Kikuchi

  9. Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-0815, Japan

    Kumiko Yoshimatsu

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  1. Zhouoxing Wei
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  2. Kenta Shimizu
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Contributions

ZW: preparation of antigens, performance of all experimental work, analysis of data, assembly of data, and writing of the manuscript. KS, YT, JA: providing expert knowledge on hantaviruses, design of experiments, and writing of the manuscript. KN: cloning and expression of Asama virus N protein. LG and NN: diagnosis of CKD and collection of patient and control sera. YS, DSM, CDG, and SMWL: collection of human sera from healthy patients. FK, KT-T, MS, and SM: preparation of Altai virus and Seewis virus cDNA and amplification of their N protein coding region. SA and KY: conception of this study, design of experiments, analysis of data, and editing of the manuscript.

Corresponding author

Correspondence to Kumiko Yoshimatsu.

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The authors declare that there is no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Written informed consent was obtained from all individual participants included in the study.

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Wei, Z., Shimizu, K., Nishigami, K. et al. Serological methods for detection of infection with shrew-borne hantaviruses: Thottapalayam, Seewis, Altai, and Asama viruses. Arch Virol 166, 275–280 (2021). https://doi.org/10.1007/s00705-020-04873-3

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