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Epidemiology, molecular characterization, and recombination analysis of chicken anemia virus in Guangdong province, China

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Abstract

Chicken anemia virus (CAV) causes severe anemia and immunosuppression in young chickens and a compromised immune response in older birds, resulting in great economic losses to the poultry industry worldwide. Here, we report the molecular epidemiology and characterization of CAV circulating in poultry in Guangdong province, China. Ninety-one of 277 chickens collected from 2016 to 2017 were CAV positive. Full-genome sequencing revealed the presence of eight separate strains. Phylogenetic analysis based on the genome sequences obtained in this study and related sequences available in the GenBank database showed that all of the CAV isolates exhibit a close relationship to each other and belong to the same genotypic group. Putative recombination events were also detected in the genomes of the newly isolated CAVs. Collectively, our findings underscore the importance of CAV surveillance and provide information that will lead to a better understanding of the evolution of CAV.

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Acknowledgements

This work was supported by the Natural Science Foundation of Guangdong Province (Grant no. 2018A030310206) and the Construction Project of Modern Agricultural Science and Technology Innovation Alliance in Guangdong Province (2018LM1112). We thank Dr. Yungfu Chang at Cornell University for critical reading of the manuscript.

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Author notes

  1. Chen Tan and Zhanxin Wang contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center, College of Animal Science, South China Agricultural University, Guangzhou, 510642, P.R. China

    Chen Tan, Xiaoya Lei, Junpeng Lu, Feng Chen, Qingmei Xie & Wencheng Lin

  2. Wen’s Group Academy, Wen’s Foodstuffs Group Co., Ltd., Xinxing, 527400, Guangdong, PR China

    Zhanxin Wang, Zhuanqiang Yan & Jianping Qin

  3. Key Laboratory of Healthy Animal Husbandry and Environmental Control of Guangdong Province, Guangzhou, 510642, Guangdong, P.R. China

    Zhanxin Wang, Junpeng Lu, Zhuanqiang Yan, Jianping Qin, Feng Chen, Qingmei Xie & Wencheng Lin

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  1. Chen Tan
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Correspondence to Qingmei Xie or Wencheng Lin.

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The authors declare no competing financial interests.

Ethical approval

All animal experiments were approved by the Committee of the Ethics on Animal Care and Experiments at South China Agricultural University (approval ID: SYXK-2014-0136). Permission was issued for the field studies, and all of the owners of the farms gave informed consent to conduct the study on this site. The animal experiments were performed in strict compliance with the Guideline for the Care and Use of Laboratory Animals of the Ministry of Science and Technology of the People’s Republic of China.

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Tan, C., Wang, Z., Lei, X. et al. Epidemiology, molecular characterization, and recombination analysis of chicken anemia virus in Guangdong province, China. Arch Virol 165, 1409–1417 (2020). https://doi.org/10.1007/s00705-020-04604-8

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  • DOI: https://doi.org/10.1007/s00705-020-04604-8

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