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Cobalt oxyhydroxide nanosheet–modulated ratiometric fluorescence platform for the selective detection of malachite green in fish

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Abstract

A ratiometric fluorescence platform was developed based on the cobalt oxyhydroxide (CoOOH) nanosheet–modulated fluorescence response of blue emissive copper nanoclusters (Cu NCs) and yellow emissive o-phenylenediamine (OPD). CoOOH nanosheets showed dual function of strong absorption and oxidation ability, which can effectively quench the blue fluorescence of Cu NCs, with an excitation and emission peak maximum at 390 and 450 nm, respectively , and transfer the OPD into yellow fluorescence products, with an excitation and emission peak maximum at 390 and 560 nm, respectively. Upon introducing butyrylcholinesterase (BChE) and its substrates, CoOOH nanosheets were decomposed into Co2+, and malachite green (MG) showed strong inhibition ability to this  process. This resulted in the obvious difference on the ratio of blue and yellow fluorescence recorded on the system in the presence and absence of MG, which was utilized for the quantitative detection of MG, with a limit of detection of 0.140 μM and a coefficient of variation of 3.5%. The fluorescence ratiometric assay showed excellent detection performances in practical sample analysis.

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Funding

This work was financially supported by the National Natural Science Foundation of China (22175052), Science Fund for Creative Research Groups of Nature Science Foundation of Hebei Province (B2021201038), Central Government Guided Local Science and Technology Development Fund (Hebei, 236Z0602G), and Research Innovation Team of College of Chemistry and Environmental Science of Hebei University (hxkytd-py2101).

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  1. State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Materials Science, Hebei University, Baoding, 071002, People’s Republic of China

    Yi Zhang, Yu-e Shi, Qian Song & Zhenguang Wang

  2. College of Pharmaceutical Science, Hebei University, Baoding, 071002, People’s Republic of China

    Shuaijing Wang & Wei Li

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  1. Yi Zhang
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Zhang, Y., Shi, Ye., Wang, S. et al. Cobalt oxyhydroxide nanosheet–modulated ratiometric fluorescence platform for the selective detection of malachite green in fish. Microchim Acta 191, 119 (2024). https://doi.org/10.1007/s00604-024-06200-y

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