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“One suction and one extrusion” mode-based wash-free platform for determination of breast cancer cell-derived exosomes

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Abstract

A simple and wash-free POCT platform based on microcapillary was developed, using breast cancer cell-derived exosomes as a model. This method adopted the “one suction and one extrusion” mode. The hybridized complex of epithelial cell adhesion molecule (EpCAM) aptamer and complementary DNA-horseradish peroxidase conjugate (CDNA-HRP) was pre-modified on the microcapillary’s inner surface. “One suction” meant inhaling the sample into the functionalized microcapillary. The exosomes could specifically bind with the EpCAM aptamer on the microcapillary’s inner wall, and then the CDNA-HRP complex was released. “One extrusion” referred to squeezing the shedding CDNA-HRP into the 3,3′,5,5′-tetramethylbenzidine (TMB)/H2O2 solution, and then the enzyme-catalyzed reaction would occur to make the solution yellow using sulfuric acid as the terminator. Therefore, exosome detection could be realized. The limit of detection was 2.69 × 104 particles mL−1 and the signal value had excellent linearity in the concentration range from 2.75 × 104 to 2.75 × 108 particles⋅mL-1 exosomes. In addition, the wash-free POCT platform also displayed a favorable reproducibility (RSD = 2.9%) in exosome detection. This method could effectively differentiate breast cancer patients from healthy donors. This work provided an easy-to-operate method for detecting cancer-derived exosomes without complex cleaning steps, which is expected to be applied to breast cancer screening.

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Acknowledgements

This work was supported from the National Natural Science Foundation of China (22074032, 22074030), National Key Research and Development Program of China (2020YFA0907500), the Key Research and Development Program of Hunan Province of China (2022GK2017), and the Natural Science Foundation of Hunan Province (2022JJ40248).

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Authors and Affiliations

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, 410082, People’s Republic of China

    Ling Xia, Mingwan Zhang, Wenjing Mei, Hongqiang Wang, Liyuan Zou, Qing Wang, Xiaohai Yang & Kemin Wang

  2. Department of Cancer Prevention and Control, Hunan Cancer Hospital/the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China

    Yingyun Hu

  3. Translational Medicine Centre, Hunan Cancer Hospital/the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China

    Ying Long

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  1. Ling Xia
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Correspondence to Qing Wang or Xiaohai Yang.

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Xia, L., Zhang, M., Hu, Y. et al. “One suction and one extrusion” mode-based wash-free platform for determination of breast cancer cell-derived exosomes. Microchim Acta 190, 322 (2023). https://doi.org/10.1007/s00604-023-05898-6

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  • DOI: https://doi.org/10.1007/s00604-023-05898-6

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