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Determination of β-glucosidase activity using single-particle enumeration with Au@CeO2 nanoparticles

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Abstract

A visible and sensitive assay for the quantitative detection of β-glucosidase (β-glu) activity based on Au@CeO2 core-shell nanoparticles (Au@CeO2 NPs) is described. As a hydrolytic enzyme, β-glu can promote the hydrolysis of β-arbutin to hydroquinone (HQ), which can trigger the decomposition of the CeO2 shell. With the single-particle enumeration (SPE) strategy coupled with dark field optical microscopy (DFM), an obvious color alteration of single Au@CeO2 NPs during the etching process can be observed in real-time. By statistically calculating the number of the etched nanoparticles, the β-glu activity level can be quantified accurately. This assay displays a broad linear range from 0.5 to 50 mU⋅mL−1 and low detection limit of 0.12 mU⋅mL−1. In addition, this method was successfully used to determine β-glu in real samples and acquires satisfactory recoveries in the range of 97.1-102.0%. This study provides a visualization analysis method for β-glu, which may be helpful for monitoring other targets in the future.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by the Scientific Research Foundation of Hunan Provincial Education Department (20A299), Open Research Fund of School of Chemistry and Chemical Engineering, Henan Normal University (2022A04), and Natural Science Foundation of Hunan Province (2022JJ40266).

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, National and Local Joint Engineering Laboratory for New Petrochemical Materials and Fine Utilization of Resources, College of Chemical Engineering, Hunan Normal University, Changsha, 410081, People’s Republic of China

    Xiang Yuan, Huiling Zhang, Huijuan Cao & Lin Wei

  2. Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Guojiang Mao

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  1. Xiang Yuan
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  2. Huiling Zhang
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Correspondence to Lin Wei.

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Yuan, X., Zhang, H., Cao, H. et al. Determination of β-glucosidase activity using single-particle enumeration with Au@CeO2 nanoparticles. Microchim Acta 189, 480 (2022). https://doi.org/10.1007/s00604-022-05580-3

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