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Single Particle Colorimetric Acid Phosphatase Activity Assay with CeO2-modified Gold Nanoparticles

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Chemical Research in Chinese Universities Aims and scope

Abstract

Acid phosphatase (ACP) is a ubiquitous phosphatase in living organisms. The abnormal variation of ACP is related to various diseases. Herein, we propose a colorimetric method based on CeO2-modified gold core shell nanoparticles (Au@CeO2 NPs) to analyze ACP activity with high sensitivity and specificity. In this design, 2-phospho-L-ascorbic acid trisodium salt (AAP) is dephosphorylated by ACP and produces reductive ascorbic acid (AA), which makes the CeO2 shell decomposition. A remarkable blue shift of localized surface plasmon resonance peak (LSPR, from yellow to green) along with the scattering intensity ratio changes from individual Au@CeO2 NPs are observed. ACP activity can be quantified by calculating the ratio changes of individual Au@CeO2 NPs. This assay reveals limit of detection (LOD) of 0.044 mU/mL and the linear range of 0.05–5.0 mU/mL, which are much lower than most of spectroscopic measurements in bulk solution. Furthermore, the recovery measurements in real samples are satisfactory and the capacity for practical application is demonstrated. As a consequence, Au@CeO2 NPs used in this assay will find new applications for the ultrasensitive detection of enzyme activity.

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Acknowledgements

This work was supported by the Natural Science Foundation of Hunan Province, China (No. 2022JJ40266) and the Open Research Fund of School of Chemistry and Chemical Engineering, Henan Normal University, China (No. 2022A04).

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, National and Local Joint Engineering Laboratory for New Petrochemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, P. R. China

    Tang Jiayao Zhu, Xiang Yuan & Lin Wei

  2. Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, P. R. China

    Guojiang Mao

  3. College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, P. R. China

    Lehui Xiao

Authors
  1. Tang Jiayao Zhu
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  2. Xiang Yuan
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  3. Guojiang Mao
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  4. Lin Wei
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Correspondence to Lin Wei.

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XIAO Lehui is a youth executive editorial board member for Chemical Research in Chinese Universities and was not involved in the editorial review or the decision to publish this article. The authors declare no conflicts of interest.

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Zhu, T.J., Yuan, X., Mao, G. et al. Single Particle Colorimetric Acid Phosphatase Activity Assay with CeO2-modified Gold Nanoparticles. Chem. Res. Chin. Univ. 40, 320–325 (2024). https://doi.org/10.1007/s40242-024-4024-6

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  • DOI: https://doi.org/10.1007/s40242-024-4024-6

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