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Portable electrochemical micro-workstation platform for simultaneous detection of multiple Alzheimer’s disease biomarkers

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Abstract

Alzheimer’s disease, as a most prevalent type of dementia, is quickly becoming one of the most expensive, lethal, and burdening diseases of this century. Though there are still no efficient therapies, early diagnosis and intervention are important directive significance to clinical works. Here, we develop a portable electrochemical micro-workstation platform consisting of an electrochemical micro-workstation and integrated electrochemical microarray for simultaneously detecting multiple AD biomarkers including Aβ40, Aβ42, T-tau, and P-tau181 in serum. The integrated electrochemical microarray is mainly used for droplet sample manipulation and signal generation. The micro-workstation can regulate signals and transfer the signals to a smartphone by Bluetooth embedded inside. This portable electrochemical micro-workstation platform exhibits excellent analysis performance. The LODs for Aβ40, Aβ42, T-tau, and P-tau181 are 0.125 pg/mL, 0.089 pg/mL, 0.142 pg/mL, and 0.176 pg/mL, respectively, which satisfies the needs of detecting AD biomarkers in serum. The combination of portable micro-workstation and integrated electrochemical microarray provides a promising strategy for the early diagnosis of Alzheimer’s disease and personal healthcare.

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Acknowledgements

We thank the Instrumental Analysis Center of Shenzhen University (Xili Campus) for providing access to the instruments used in the experiments.

Funding

The work was supported by Longgang District Medical and health science and technology project (LGKCYLWS2021000003), the National Natural Science Foundation of China (21804007, 21890742), SZU Top Ranking Project (86000000210), Shenzhen Key Laboratory for Nano-Biosensing Technology (ZDSYS20210112161400001), and Shenzhen Stability Support Plan (20200806163622001).

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Authors and Affiliations

  1. Longgang District Central Hospital of Shenzhen, Shenzhen, China

    Yibiao Liu, Zhen Huang & Lingyan Zhang

  2. Office of Shenzhen Clinical College, Guangzhou University of Chinese Medicine, Longggang District Central Hospital, Shenzhen, China

    Zhen Huang

  3. School of Biomedical Engineering, Health Science Center, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, Guangdong, China

    Qing Xu, Qiong Liu & Tailin Xu

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  1. Yibiao Liu
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  2. Zhen Huang
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  3. Qing Xu
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  4. Lingyan Zhang
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Correspondence to Qiong Liu or Tailin Xu.

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Yibiao Liu, Zhen Huang, and Qing Xu are co-first authors

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Liu, Y., Huang, Z., Xu, Q. et al. Portable electrochemical micro-workstation platform for simultaneous detection of multiple Alzheimer’s disease biomarkers. Microchim Acta 189, 91 (2022). https://doi.org/10.1007/s00604-022-05199-4

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