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UV-assisted one-pot synthesis of bimetallic Ag-Pt decorated reduced graphene oxide for colorimetric determination of hydrogen peroxide

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Abstract

Bimetallic Ag-Pt nanoparticles decorated on the surface of reduced graphene oxide (Ag-Pt/rGO) were designed and selected as a nanozyme for the assay of hydrogen peroxide. The nanocomposites were prepared through a one-pot reduction of potassium chloroplatinate, silver nitrate, and graphene oxide under ultraviolet irradiation without using any extra chemical reducing agents or surfactants. The successful formation of Ag-Pt/rGO nanocomposites was confirmed by transmission electron microscopy, energy disperse spectroscopy mapping, X-ray photoelectron spectroscopy, and X-ray diffraction analysis. Significantly, Ag-Pt/rGO nanocomposites possessed excellent peroxidase-like activity toward the catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine to form a blue product in the presence of hydrogen peroxide. Steady-state kinetics studies suggested that Ag-Pt/rGO nanocomposites had high affinity to hydrogen peroxide. Based on these properties, a convenient and sensitive method for the colorimetric determination of hydrogen peroxide was developed. Under optimal conditions, the absorbance at 652 nm increases linearly in the 10–100 μM and 100 μM–1 mM ranges of hydrogen peroxide concentration, and the detection limit is 0.9 μM (S/N = 3). The method was successfully applied to the determination of hydrogen peroxide in real water samples.

Ag-Pt/rGO nanocomposites were prepared by a one-pot UV irradiation method and used as a novel nanozyme for colorimetric determination of H2O2.

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Funding

This work was supported by the National Natural Science Foundation of China (21675139, 21705140, 21876144, 21974120, 21906142), the Natural Science Foundation of Jiangsu Province (BK20170474, BK20191479), and Qing Lan Project of Jiangsu universities.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China

    Lei Yao

  2. School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, 224051, China

    Fen-Ying Kong, Zhong-Xia Wang, Heng-Ye Li, Rui Zhang, Hai-Lin Fang & Wei Wang

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  1. Lei Yao
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  2. Fen-Ying Kong
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  7. Wei Wang
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Correspondence to Fen-Ying Kong or Wei Wang.

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Yao, L., Kong, FY., Wang, ZX. et al. UV-assisted one-pot synthesis of bimetallic Ag-Pt decorated reduced graphene oxide for colorimetric determination of hydrogen peroxide. Microchim Acta 187, 410 (2020). https://doi.org/10.1007/s00604-020-04350-3

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