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Target-responsive ratiometric fluorescent aptasensor for OTA based on energy transfer between [Ru(bpy)3]2+ and silica quantum dots

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Abstract

A ratiometric fluorescent aptasensor based on energy transfer between [Ru(bpy)3]2+ and silica quantum dots (silica QDs) for assaying OTA was fabricated. The aptamer for OTA was used as the gate to shield the fluorescent reagent [Ru(bpy)3]2+ into mesoporous silica nanoparticle (MSN). In the presence of OTA, the constrained [Ru(bpy)3]2+ was released from MSN due to a target-induced aptamer conformational change. The released [Ru(bpy)3]2+ adsorbed onto the negatively charged silica QDs through electrostatic interaction. This creates appearance of fluorescence from [Ru(bpy)3]2+ at 625 nm and decrease of the fluorescence from silica QDs at 442 nm owing to the energy transfer. The value of FL625nm/FL442nm was in proportion to the concentration of OTA in the range 0.5~100 ng mL−1 with a LOD of 0.08 ng mL−1. Practical applicability of this method was validated by the determination of OTA in flour samples.

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Acknowledgments

H. Xu and X. Zhu thank the Natural Science Funds of Fujian Province for Distinguished Young Scholar (2019J06021) and the University Distinguished Young Research Talent Training Program of Fujian Province, respectively.

Funding

This project was financially supported by NSFC (81773894, 21305014, and 21677033), Fujian Provincial Department of Science and Technology, China (grant number 2017Y0002), and FJ 2011 Program (grant number 2015-75).

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Authors and Affiliations

  1. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Xi Zhu, Wenjing Li, Liping Lin, Xiaojuan Huang & Guidi Yang

  2. Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, 35002, Fujian, People’s Republic of China

    Xi Zhu, Wenjing Li & Guidi Yang

  3. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, People’s Republic of China

    Huifeng Xu

  4. Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, People’s Republic of China

    Huifeng Xu

  5. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, People’s Republic of China

    Zhenyu Lin

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  1. Xi Zhu
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Correspondence to Huifeng Xu or Guidi Yang.

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Zhu, X., Li, W., Lin, L. et al. Target-responsive ratiometric fluorescent aptasensor for OTA based on energy transfer between [Ru(bpy)3]2+ and silica quantum dots. Microchim Acta 187, 270 (2020). https://doi.org/10.1007/s00604-020-04245-3

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