Abstract
This study was aimed at assessing the ability of the antimicrobial protein (AMP) produced by Bacillus amyloliquefaciens MBL27 as a potent wound healant. Rat models were used to study the efficacy of AMP and AMP-incorporated chitosan sheet along with control groups. AMP and AMP-incorporated chitosan sheet significantly improved wound contraction when compared to controls. Rate of wound contraction (97.23%), decreased period of epithelialization (14 days), and the levels of biochemical markers such as hydroxyproline (collagen), total protein, uronic acid, and hexosamine in the granulation tissue on different days of wound healing revealed the wound healing efficacy of the AMP. The histological examinations also correlated well with the biochemical findings, confirming the wound healing efficacy of the AMP. The results indicate the beneficial effects of AMP from B. amyloliquefaciens MBL27 and it is prospective to be developed into novel therapeutic agent for dermal wound healing.
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Acknowledgements
The authors wish to thank Dr. Hilda, Microbiology Laboratory CLRI, for the support in carrying out this animal experiment.
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This work was supported by the Council of Scientific and Industrial Research (CSIR) (grant number A/6(276)/2008/EMR-I dated 15.03.2008).
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Dr. K. Vijayalakshmi - methodology, conceptualization, practical investigation, formal analysis, writing - original draft. Dr. G. Suseela Rajakumar - editing and review of the draft, supervision, validation.
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The Institutional Animal Ethics Committee (IAEC) constituted by Central Leather Research Institute, Chennai, India approved all the protocols of animal experiments of this study (IAEC Reg. No. 06/002/08).
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K, V., G, S.R. Experimental evaluation of an antimicrobial protein from Bacillus amyloliquefaciens MBL27 for wound healing potential in rats. Comp Clin Pathol 31, 627–647 (2022). https://doi.org/10.1007/s00580-022-03361-1
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DOI: https://doi.org/10.1007/s00580-022-03361-1