Abstract
Purpose
Colorectal cancer (CRC) is the fourth-most common cancer worldwide and the second most common cancer cause of death in the world. The components of the TGFβ-signalling pathway, which are often affected by miRNAs, are involved in the regulation of apoptosis and cell cycle. Therefore, in the current study, the expression of BMP2 gene in CRC tissues at different clinical stages compared to the non-tumour tissues has been assessed. Moreover, the plasma BMP2 protein concentration in the same group of CRC patients has been validated. Due to the constant necessity to conduct further research of the correlation between specific miRNAs and mRNAs in CRC, in silico analysis has been performed to select miRNAs that regulate BMP2 mRNA.
Methods
The cDNA samples from tumor and non-tumor tissue were used in a qPCR reaction to determine the mRNA expression of the BMP2 gene and the expression of selected miRNAs. The concentration of BMP2 protein in plasma samples was also measured.
Results
It was indicated that BMP2 was downregulated in CRC tissue. Moreover, miR-370 and miR-138 expression showed an upward trend. Decreased BMP2 with accompanied increasing miR-370 and miR-138 expression was relevant to the malignant clinicopathological features of CRC and consequently poor patient prognosis.
Conclusion
Our data suggest that miR-370 with its clear expression in plasma samples may be a potential diagnostic marker to determine the severity of the disease in patients at a later stage of colorectal cancer.
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Data availability
The data that support the findings of this study areavailable from the corresponding author upon reasonable request.
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Project financed under the program of the Minister of Science and Higher Education called “Regional Initiative of Excellence” in the years 2019-2022, project no. 024/RID/2018/19.
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Conceptualization, SG and JG; methodology, AP and MK; data analysis and interpretation, MKD, CKR and AP; investigation, MKD, CKR and BSM; writing—original draft preparation, AP and MKD; writing—review and editing, SG and BSM; supervision, SG and JG; funding acquisition, SG. All authors discussed the results and contributed to the final manuscript.
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Piechowska, A., Kruszniewska-Rajs, C., Kimsa-Dudek, M. et al. The role of miR-370 and miR-138 in the regulation of BMP2 suppressor gene expression in colorectal cancer: preliminary studies. J Cancer Res Clin Oncol 148, 1569–1582 (2022). https://doi.org/10.1007/s00432-022-03977-4
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DOI: https://doi.org/10.1007/s00432-022-03977-4