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Up-regulation of miR-182 expression in colorectal cancer tissues and its prognostic value

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Purpose

Accumulating evidences indicate that dysregulated microRNAs (miRNA) are involved in cancer tumorigenesis and progression. In the present study, we evaluated the expression of miR-182 in colorectal cancer and adjacent noncancerous tissues and explored its associations with clinicopathological characteristics and prognosis.

Methods

Quantitative real-time PCR was used to analyze the expression of miR-182 in 148 pairs of colorectal cancer and adjacent noncancerous tissues. The relationship between miR-182 expression and clinicopathological characteristics in colorectal cancer tissues was estimated using Mann–Whitney U test or Kruskal–Wallis test, as appropriate. We calculated the survival curves and prognostic values of each variable by the Kaplan–Meier method and Cox proportional hazards regression analysis, respectively.

Results

The expression of miR-182 was found up-regulated in colorectal cancer tissues compared with adjacent noncancerous tissues (p < 0.001), and its up-regulation was significantly correlated with large tumor size (p = 0.016), positive regional lymph node metastasis (p = 0.008), and advanced tumor–node–metastasis stage (p = 0.020). Furthermore, Kaplan–Meier analysis demonstrated that high miR-182 expression predicted poor survival (p = 0.001), and Cox proportional hazards risk analysis indicated that miR-182 was an independent prognostic factor for colorectal cancer.

Conclusions

MiR-182 was up-regulated in colorectal cancer tissues and correlated with adverse clinical characteristics and poor prognosis, indicating that miR-182 might be involved in colorectal cancer progression and could be used as a potential prognostic biomarker and therapeutic target in the management of colorectal cancer.

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Acknowledgments

This study was supported by the Shandong Province Natural Science Foundation (no. ZR2010H004) and the National Key Clinical Medical Specialties Foundation. We thank Shuo Chen for providing colon cancer cells.

Conflict of interest

The authors have no conflict of interest.

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Authors and Affiliations

  1. Department of Clinical Laboratory, Qilu Hospital, Shandong University, 107 Wenhua West Road, Jinan, Shandong, 250012, People’s Republic of China

    Hui Liu, Lutao Du, Yongmei Yang, Juan Li, Lili Wang, Xin Zhang, Yimin Liu, Zhaogang Dong, Wei Li, Guixi Zheng & Chuanxin Wang

  2. Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, People’s Republic of China

    Zhihua Wen

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  1. Hui Liu
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  2. Lutao Du
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  5. Juan Li
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  10. Wei Li
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Corresponding author

Correspondence to Chuanxin Wang.

Additional information

Hui Liu and Lutao Du contributed equally to this article.

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Liu, H., Du, L., Wen, Z. et al. Up-regulation of miR-182 expression in colorectal cancer tissues and its prognostic value. Int J Colorectal Dis 28, 697–703 (2013). https://doi.org/10.1007/s00384-013-1674-0

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  • DOI: https://doi.org/10.1007/s00384-013-1674-0

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