Abstract
Main conclusion
Flavonoids are involved in axillary bud development in upland cotton. The phenylpropanoid and flavonoid biosynthesis pathways regulate axillary bud growth by promoting the transport of auxin in upland cotton.
Abstract
In cotton production, simplified cultivation and mechanical harvesting are emerging trends that depend on whether the cotton plant type meets production requirements. The axillary bud is an important index of cotton plant-type traits, and the molecular mechanism of axillary bud development in upland cotton has not yet been completely studied. Here, a combined investigation of transcriptome and metabolome analyses in G. hirsutum CCRI 117 at the fourth week (stage 1), fifth week (stage 2) and sixth week (stage 3) after seedling emergence was performed. The metabolome results showed that the total lipid, amino acid and organic acid contents in the first stalk node decreased during axillary bud development. The abundance of 71 metabolites was altered between stage 2 and stage 1, and 32 metabolites exhibited significantly altered abundance between stage 3 and stage 2. According to the correlation analysis of metabolome and transcriptome profiles, we found that phenylpropanoid and flavonoid biosynthesis pathways exhibit high enrichment degrees of both differential metabolites and differential genes in three stages. Based on the verification of hormone, soluble sugar and flavonoid detection, we propose a model for flavonoid-mediated regulation of axillary bud development in upland cotton, revealing that the decrease in secondary metabolites of phenylpropanoid and flavonoid biosynthesis is an essential factor to promote the transport of auxin and subsequently promote the growth of axillary buds. Our findings provide novel insights into the regulation of phenylpropanoid and flavonoid biosynthesis in axillary bud development and could prove useful for cultivating machine-harvested cotton varieties with low axillary buds.
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Abbreviations
- GO:
-
Gene ontology
- DEG:
-
Differentially expressed gene
- ZT:
-
Zeatin
- GA:
-
Gibberellin
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Acknowledgements
This work was supported by Natural Science Foundation of Henan Province of China (212300410308); Xinjiang Production and Construction Corps Science and Technology NOVA Program (2020CB029); Ministry of Finance and Ministry of Agriculture and Rural Affairs: National Modern Agricultural Industry Technology System of China; Program for Key Areas of Science and Technology of Xinjiang Production and Construction Corps Third Division and Tumsuk City (KY2021GG08).
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425_2021_3597_MOESM1_ESM.xlsx
Supplementary file1. Fig. S1 Principal component analysis (PCA) plot of metabolome differences between three stages of axillary bud development in CCRI 117. Fig. S2 GO classification of assembled unigenes. X-axis: the major categories of biological process, molecular function and cellular component. Y-axis: the − log10 (P value) of unigene annotated in the category. Fig. S3 GO function classification of DEGs. X-axis: the numbers of DEGs. Y-axis: GO classification of DEGs. (XLSX 19623 kb)
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Shi, J., Zhou, H., Liu, X. et al. Correlation analysis of the transcriptome and metabolome reveals the role of the flavonoid biosynthesis pathway in regulating axillary buds in upland cotton (Gossypium hirsutum L.). Planta 254, 7 (2021). https://doi.org/10.1007/s00425-021-03597-1
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DOI: https://doi.org/10.1007/s00425-021-03597-1