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Constitutive expression of CaSRP1, a hot pepper small rubber particle protein homolog, resulted in fast growth and improved drought tolerance in transgenic Arabidopsis plants

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Abstract

Transient and long-term shortages of fresh water are major adverse environmental factors that cause dramatic reductions in crop production and distribution globally. In this study, we isolated a full-length CaSRP1 (Capsicum annuum stress-related protein 1) cDNA, which was rapidly induced by dehydration in hot pepper plants. The predicted CaSRP1 protein sequence exhibited significant amino acid identity to putative stress-related proteins and the small rubber particle protein (SRPP) found in rubber trees (Hevea brasiliensis). To study the cellular functions of CaSRP1, transgenic Arabidopsis plants (35S:CaSRP1) that constitutively expressed the CaSRP1 gene were constructed. Overexpression of CaSRP1 resulted in enhanced root and shoot growth and earlier bolting in the transgenic plants relative to wild-type plants. In addition, 35S:CaSRP1 overexpressors exhibited enhanced tolerance to drought stress as compared to the control plants. These results suggest that CaSRP1 plays dual functions as a positive factor for tissue growth and development and for drought-defensive responses. A possible cellular function of SRPP homologs in non-rubber-producing plants in relation to drought stress tolerance is discussed.

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Abbreviations

CaSRP1:

Capsicum annuum stress-related protein 1

DW:

Dry weight

GHS:

Guayule homolog of SRPP

PCR:

Polymerase chain reaction

SRPP:

Small rubber particle protein

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Acknowledgments

This work was supported by grants from the Technology Development Program for Agriculture and Forestry (Project No. 309017-5 funded by the Ministry for Agriculture, Forestry and Fisheries, Republic of Korea), the National Research Foundation (Project No. 2009-0078317 funded by the Ministry of Education, Science, and Technology, Republic of Korea), and the BioGreen 21 Program (funded by the Rural Development Administration).

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Authors and Affiliations

  1. Department of Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea

    Eun Yu Kim, Young Sam Seo, Hanna Lee & Woo Taek Kim

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  1. Eun Yu Kim
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  2. Young Sam Seo
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  3. Hanna Lee
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  4. Woo Taek Kim
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Corresponding author

Correspondence to Woo Taek Kim.

Additional information

The nucleotide sequence data reported here has been deposited in the GenBank database under accession number GU373985 (CaSRP1).

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Kim, E.Y., Seo, Y.S., Lee, H. et al. Constitutive expression of CaSRP1, a hot pepper small rubber particle protein homolog, resulted in fast growth and improved drought tolerance in transgenic Arabidopsis plants. Planta 232, 71–83 (2010). https://doi.org/10.1007/s00425-010-1149-2

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