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Endogenous ion channels expressed in human embryonic kidney (HEK-293) cells

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Abstract

Mammalian expression systems, particularly the human embryonic kidney (HEK-293) cells, combined with electrophysiological studies, have greatly benefited our understanding of the function, characteristic, and regulation of various ion channels. It was previously assumed that the existence of endogenous ion channels in native HEK-293 cells could be negligible. Still, more and more ion channels are gradually reported in native HEK-293 cells, which should draw our attention. In this regard, we summarize the different ion channels that are endogenously expressed in HEK-293 cells, including voltage-gated Na+ channels, Ca2+ channels, K+ channels, Cl− channels, nonselective cation channels, TRP channels, acid-sensitive ion channels, and Piezo channels, which may complicate the recording of the heterogeneously expressed ion channels to a certain degree. We noted that the expression patterns and channel profiles varied with different studies, which may be due to the distinct originality of the cells, cell culture conditions, passage numbers, and different recording protocols. Therefore, a better knowledge of endogenous ion channels may help minimize potential problems in characterizing heterologously expressed ion channels. Based on this, it is recommended that HEK-293 cells from unknown sources should be examined before transfection for the characterization of their functional profile, especially when the expression level of exogenous ion channels does not overwhelm the endogenous ion channels largely, or the current amplitude is not significantly higher than the native currents.

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Funding

This work was generously supported by grant from the National Natural Science Foundation of Heilongjiang Province of China (QC2018094).

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Authors and Affiliations

  1. School of Biomedical Sciences and Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, Hong Kong, China

    Jun Zhang & Xiaoqiang Yao

  2. Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China

    Huikai Yuan

  3. Department of Biopharmaceutical Sciences, School of Pharmacy, Harbin Medical University at Daqing, No. 39 Xinyang Rd, High-tech District, Daqing, 163319, Heilongjiang Province, China

    Shuo Chen

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  1. Jun Zhang
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Zhang, J., Yuan, H., Yao, X. et al. Endogenous ion channels expressed in human embryonic kidney (HEK-293) cells. Pflugers Arch - Eur J Physiol 474, 665–680 (2022). https://doi.org/10.1007/s00424-022-02700-z

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