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Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

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Abstract

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Feng You  (尤锋), Wei Wang  (王伟), Dongdong Xu  (徐冬冬), Xiangping Zhu  (朱香萍), Jing Ni  (倪静), Zhihao Wu  (吴志昊), Yongli Xu  (徐永立), Xincheng Wang  (王新成) & Peijun Zhang  (张培军)

  2. Qingdao Agricultural University, Qingdao, 266109, China

    Xiangping Zhu  (朱香萍)

Authors
  1. Feng You  (尤锋)
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  2. Wei Wang  (王伟)
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  3. Dongdong Xu  (徐冬冬)
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  4. Xiangping Zhu  (朱香萍)
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  5. Jing Ni  (倪静)
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  6. Zhihao Wu  (吴志昊)
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  7. Yongli Xu  (徐永立)
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  8. Xincheng Wang  (王新成)
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  9. Peijun Zhang  (张培军)
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Correspondence to Feng You  (尤锋).

Additional information

Supported by National Natural Science Foundation of China (No. 30571445), National High-Technology Research and Development Program (863 Program, No. 2006AA10A404), and Project from the Ministry of Science and Technology of China (No. 2006DKA30470-017)

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You, F., Wang, W., Xu, D. et al. Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses. Chin. J. Ocean. Limnol. 27, 317–323 (2009). https://doi.org/10.1007/s00343-009-9134-y

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  • DOI: https://doi.org/10.1007/s00343-009-9134-y

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