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In vitro and in vivo biocompatibility study on laser 3D microstructurable polymers

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Abstract

Films and microstructured scaffolds have been fabricated using direct laser writing out of different polymers: hybrid organic-inorganic ORMOCORE b59, acrylate-based AKRE23, novel organic-inorganic Zr containing hybrid SZ2080, and biodegradable PEG-DA-258. Adult myogenic stem cells were grown on these surfaces in vitro. Their adhesion, growth, and viability test results suggest good potential applicability of the materials in biomedical practice. Pieces of these polymers were implanted in rat’s paravertebral back tissue. Histological examination of the implants and surrounding tissue ex vivo after 3 weeks of implantation was conducted and results show the materials to be at least as biocompatible as surgical clips or sutures. The applied direct laser writing technique seems to offer good future prospects in a polymeric 3D scaffold design for artificial tissue engineering with autologous stem cells.

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Acknowledgements

This work was supported by the Lithuanian Science Council grant MIP-10344 (Creation of Artificial Tissues for Regenerative Medicine). Domas Paipulas and Gabija Bickauskaite (VU LRC) are acknowledged for image digital processing and proofreading.

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Authors and Affiliations

  1. Laser Research Center, Department of Quantum Electronics, Physics Faculty, Vilnius University, Sauletekio ave. 10, 10223, Vilnius, Lithuania

    Mangirdas Malinauskas, Paulius Danilevicius, Albertas Zukauskas, Evaldas Balciunas, Vytautas Purlys, Roaldas Gadonas & Algis Piskarskas

  2. Vivarium, Institute of Biochemistry, Vilnius University, Mokslininku str. 12, 08662, Vilnius, Lithuania

    Daiva Baltriukiene, Rasa Jarasiene & Virginija Bukelskiene

  3. Centre of Heart Surgery, Vilnius University Hospital Santariskiu Klinikos, Santariskiu str. 2, 08661, Vilnius, Lithuania

    Antanas Kraniauskas, Raimondas Sirmenis & Vytautas Sirvydis

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  1. Mangirdas Malinauskas
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Correspondence to Mangirdas Malinauskas.

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Malinauskas, M., Baltriukiene, D., Kraniauskas, A. et al. In vitro and in vivo biocompatibility study on laser 3D microstructurable polymers. Appl. Phys. A 108, 751–759 (2012). https://doi.org/10.1007/s00339-012-6965-8

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