Abstract
Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using two-photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.
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This work was supported by the Russian Science Foundation, grant 15-15-00132.
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Shpichka, A., Koroleva, A., Kuznetsova, D., Dmitriev, R.I., Timashev, P. (2017). Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues. In: Dmitriev, R. (eds) Multi-Parametric Live Cell Microscopy of 3D Tissue Models. Advances in Experimental Medicine and Biology, vol 1035. Springer, Cham. https://doi.org/10.1007/978-3-319-67358-5_5
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DOI: https://doi.org/10.1007/978-3-319-67358-5_5
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