Abstract
Key message
Two peanut LEC1-type genes exhibit partial functional redundancy. AhNFYB10 could complement almost all the defective phenotypes of lec1-2 in terms of embryonic morphology, while AhNF-YB1 could partially affect these phenotypes.
Abstract
LEAFY COTYLEDON1 (LEC1) is a member of the nuclear factor Y (NF-Y) family of transcription factors and has been identified as a key regulator of embryonic development. In the present study, two LEC1-type genes from Arachis hypogeae were identified and designated as AhNF-YB1 and AhNF-YB10; these genes belong to subgenome A and subgenome B, respectively. The functions of AhNF-YB1 and AhNF-YB10 were investigated by complementation analysis of their defective phenotypes of the Arabidopsis lec1-2 mutant and by ectopic expression in wild-type Arabidopsis. The results indicated that both AhNF-YB1 and AhNF-YB10 participate in regulating embryogenesis, embryo development, and reserve deposition in cotyledons and that they have partial functional redundancy. In contrast, AhNF-YB10 complemented almost all the defective phenotypes of lec1-2 in terms of embryonic morphology and hypocotyl length, while AhNF-YB1 had only a partial effect. In addition, 30–40% of the seeds of the AhNF-YB1 transformants exhibited a decreasing germination ratio and longevity. Therefore, appropriate spatiotemporal expression of these genes is necessary for embryo morphogenesis at the early development stage and is responsible for seed maturation at the mid-late development stage. On the other hand, overexpression of AhNF-YB1 or AhNF-YB10 at the middle to late stages of Arabidopsis seed development improved the weight, oil content, and fatty acid composition of the transgenic seeds. Moreover, the expression levels of several genes associated with fatty acid synthesis and embryogenesis were significantly greater in developing AhNF-YB10-overexpressing seeds than in control seeds. This study provides a theoretical basis for breeding oilseed crops with high yields and high oil content.
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Acknowledgements
We thank Jianru Zuo (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for kindly providing us with the plasmid with 211-bp promoter of Napin A (211P) and the methods of vector construction.
Funding
This research was supported by grants from Natural Science Foundation of Shandong Province (ZR2021MC054), National Natural Science Foundation of China (30971546), Shandong Provincial Key Research and Development Program (2023LZGCQY019), Shandong Provincial Key Research and Development Program (2021LZGC025).
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GT conducted the experiments and drafted the manuscript, PX and CJ conducted the experiments, GL analyzed the data. LS and SW designed the study and revised the manuscript. All authors read and approved the final version.
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Tang, G., Xu, P., Jiang, C. et al. Peanut LEAFY COTYLEDON1-type genes participate in regulating the embryo development and the accumulation of storage lipids. Plant Cell Rep 43, 124 (2024). https://doi.org/10.1007/s00299-024-03209-8
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DOI: https://doi.org/10.1007/s00299-024-03209-8