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A comprehensive investigation of the regulatory roles of OsERF096, an AP2/ERF transcription factor, in rice cold stress response

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Abstract

Key message

OsERF096 negatively regulates rice cold tolerance and mediates IAA biosynthesis and signaling under cold stress.

Abstract

The APETALA2/ethylene-responsive factor (AP2/ERF) transcription factors play important roles in regulating plant tolerance to abiotic stress. OsERF096 was previously identified as a direct target of miR1320, and was suggested to negatively regulate rice cold tolerance. In this study, we performed RNA-sequencing and targeted metabolomics assays to reveal the regulatory roles of OsERF096 in cold stress response. GO and KEGG analysis of differentially expressed genes showed that the starch and sucrose metabolism, plant–pathogen interaction, and plant hormone signal transduction pathways were significantly enriched. Quantification analysis confirmed a significant difference in sugar contents among WT and OsERF096 transgenic lines under cold treatment. Targeted metabolomics analysis uncovered that IAA accumulation and signaling were modified by OsERF096 in response to cold stress. Expectedly, qRT-PCR assays confirmed significant OsIAAs and OsARFs expression changes in OsERF096 transgenic lines. Finally, we identified three targets of OsERF096 based on RNA-seq, qRT-PCR, and dual-LUC assays. In summary, these results revealed the multiple regulatory roles of OsERF096 in cold stress response.

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The data generated or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (32101672, U20A2025), and Key Research and Development Plan of Heilongjiang Province (GZ2022ZX02B05-2).

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  1. Xiaoxi Cai and Yue Chen have contributed equally to this work.

Authors and Affiliations

  1. Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, 163319, China

    Xiaoxi Cai, Yue Chen, Yan Wang, Yang Shen, Junkai Yang, Bowei Jia, Xiaoli Sun & Mingzhe Sun

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  1. Xiaoxi Cai
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  2. Yue Chen
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  7. Xiaoli Sun
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Contributions

XS and MS conceived and designed the experiment. XC and YC completed most of the experiments and wrote the main manuscript. YW prepared the materials for the experiment. YS and JY analyzed experimental data. BJ plotted the resulting images, Figs. 1, 2, 3, 4, 5, 6. All authors have read and approved the final manuscript.

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Correspondence to Xiaoli Sun or Mingzhe Sun.

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Cai, X., Chen, Y., Wang, Y. et al. A comprehensive investigation of the regulatory roles of OsERF096, an AP2/ERF transcription factor, in rice cold stress response. Plant Cell Rep 42, 2011–2022 (2023). https://doi.org/10.1007/s00299-023-03079-6

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