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An APETALA2/ethylene responsive factor, OsEBP89 knockout enhances adaptation to direct-seeding on wet land and tolerance to drought stress in rice

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Abstract

Water stress is the most important adverse factor limiting rice production. Too much water leads to flood and too little leads to drought. Floods and droughts can severely damage crop at different times of the rice life cycle. So the research on submergence tolerance and drought resistance of rice is particularly urgent. In this study, we reported that OsEBP89 (Oryza sativa Ethylene-responsive element binding protein, clone 89), a member of the AP2/ERF subfamily, is involved in a novel signal transduction associated with the tolerance to drought and submergence stress. OsEBP89 was found to be strongly inhibited by drought stress and promoted by submergence. The OsEBP89 protein was located at the nucleus in the rice protoplast. Loss of OsEBP89 was found to improve the seed germination under submerged conditions and also enhanced the tolerance to drought stress throughout growth stage. Additionally, OsEBP89 knockout rice plants increased the accumulation of proline, improved the ability to scavenge ROS compared to overexpression lines and wild type after PEG treatment. Transcriptome data indicates that knockout of OsEBP89 improved the expression of specific genes in response to adverse factors, such as OsAPX1, OsHsfA3, and OsP5CS. Further results indicate that OsEBP89 can interact with and be phosphorylated by SnRK1α (sucrose non-fermenting-1-related protein kinase-1 gene). These findings provide insight into the mechanism of abiotic stress tolerance, and suggest OsEBP89 as a new genetic engineering resource to improve abiotic stress tolerance in rice.

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Abbreviations

AP2/ERF:

APETALA2/ethylene responsive factor

qRT-PCR:

Quantitative real-time polymerase chain reaction

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

DEGs:

Differentially expressed genes

GO:

Gene ontology

Y2H:

Yeast TWO hybrid

3AT:

3-Aminotriazol

LCI:

Luciferase complementation imaging

IP:

Immunoprecipitation

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Acknowledgements

We thank Dr. Hao, D., from College of Agriculture & Biotechnology, Zhejiang University, for substrate phosphorylation assay.

Funding

This study was supported by the Natural Science Foundation of Shanghai (17ZR1425400), the National Key Technologies R&D Program of China (2016ZX08001003), and the Youth Talent Development Project supported by the Shanghai Municipal Agriculture Commission 2017 (1–33).

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Authors and Affiliations

  1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Yu Zhang

  2. Shanghai Agrobiological Gene Center, Shanghai, 201106, China

    Yu Zhang, Jia Li, Shoujun Chen, Xiaosong Ma, Haibin Wei, Chen Chen, Ningning Gao, Yuqiao Zou, Deyan Kong, Tianfei Li, Zaochang Liu, Shunwu Yu & Lijun Luo

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  1. Yu Zhang
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Correspondence to Shunwu Yu or Lijun Luo.

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Zhang, Y., Li, J., Chen, S. et al. An APETALA2/ethylene responsive factor, OsEBP89 knockout enhances adaptation to direct-seeding on wet land and tolerance to drought stress in rice. Mol Genet Genomics 295, 941–956 (2020). https://doi.org/10.1007/s00438-020-01669-7

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