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Antagonistic Activity of Pseudomonas fluorescens Strain X1 Against Different Fusaria and it’s In Vivo Analysis Against Fusarium udum Infected Pigeon Pea

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Abstract

A plant growth-promoting rhizobacterial strain, Pseudomonas fluorescens X1 isolated from the garden soil was employed for antagonistic activity against different species of fusaria. Strain X1 inhibited four different fusaria (Fusarium moniliforme, Fusarium oxysporum, Fusarium semitectum and Fusarium udum) in dual culture plate assay, and in broth culture using cell-free culture filtrate. Scanning electron microscopic (SEM) analysis revealed deformation and shrinkage in mycelia of fusaria after treatment with strain X1. Confocal micrographs showed degeneration of nuclei inside the cells of fusaria for the same effect. Strain X1 exhibited maximum antifungal activity, when it was grown in nutrient broth yeast (NBY) medium amended with 1 mM NH4MoO4 and 1% glucose. The antifungal extracts eluted from thin-layer chromatography (TLC) followed by high performance liquid chromatography (HPLC) showed two fractions active against different fusaria. Liquid chromatography-mass spectrometry (LCMS) analysis of the two fractions 1 and 2 corresponded to molecular ions at m/z 177.16 and m/z 177.09, respectively. Infra-red (IR) analysis showed five similar absorption bands in both the fractions analysed. In vivo analysis of strain X1 alone and along with fungicide inhibited the growth of F. udum and improved the biomass and growth of pigeon pea. These results indicated that strain X1 could be possibly used as a biocontrol agent to inhibit the growth of soil-borne diseases of different fusaria including F. udum that causes wilting in pigeon pea.

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Funding

The financial assistance provided by the Department of Science & Technology, Govt. of India under the DST project SR/SO/PS-109/06 and R&D grant by the University of Delhi are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Botany, Dyal Singh College, University of Delhi, New Delhi, 110003, India

    Nellie Laisram

  2. Department of Botany, Zakir Husain Delhi College, University of Delhi, New Delhi, 110002, India

    Zeeshanur Rahman

  3. Department of Botany, University of Delhi, New Delhi, 110007, India

    Ved Pal Singh

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  1. Nellie Laisram
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  2. Zeeshanur Rahman
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  3. Ved Pal Singh
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NL: designed and carried out the experiments and wrote the manuscript, ZR: wrote and edited the manuscript, and VPS: conceptualized the idea.

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Correspondence to Zeeshanur Rahman.

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284_2023_3184_MOESM1_ESM.pptx

Supplementary file1 (PPTX 573 KB) Supplementary Fig 1. Antifungal activity of the purified compound (s), 1 and 2 against F. moniliforme (A), F. oxysporum (B), F. semitectum (C), and F. udum (D) and c in each plate refers to control.

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Laisram, N., Rahman, Z. & Singh, V.P. Antagonistic Activity of Pseudomonas fluorescens Strain X1 Against Different Fusaria and it’s In Vivo Analysis Against Fusarium udum Infected Pigeon Pea. Curr Microbiol 80, 98 (2023). https://doi.org/10.1007/s00284-023-03184-5

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