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Screening of Thiopeptide-Producing Streptomycetes Isolated From the Rhizosphere Soil of Juniperus excelsa

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Abstract

The identification of an increasing number of drug-resistant pathogens has stimulated the development of new therapeutic agents to combat them. Microbial natural products are among the most important elements when it comes to drug discovery. Today, thiopeptide antibiotics are receiving increasing research attention due to their potent activity against Gram-positive bacteria. In this study, we demonstrated the successful use of a whole-cell microbial biosensor (Streptomyces lividans TK24 pMO16) for the specific detection of thiopeptide antibiotics among the native actinomycete strains isolated from the rhizosphere soil of Juniperus excelsa (Bieb.). Among the native strains, two strains of Streptomyces, namely sp. Je 1–79 and Je 1–613, were identified that were capable of producing thiopeptide antibiotics. A multilocus sequence analysis of five housekeeping genes (gyrB, atpD, recA, rpoB, and trpB) classified them as representatives of two different species of the genus Streptomyces. The thiopeptide antibiotics berninamycin A and B were identified in the extracts of the two strains by means of a dereplication analysis. The berninamycin biosynthetic gene cluster was also detected in the genome of the Streptomyces sp. Je 1–79 strain and showed a high level of similarity (93%) with the ber cluster from S. bernensis. Thus, the use of this whole-cell biosensor during the first stage of the screening process could serve to accelerate the specific detection of thiopeptide antibiotics.

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Acknowledgements

This work was supported by Grant N/309-2003 from the Ministry of Education and Science of Ukraine to VF, and personal Grant 91657043 from the German Academic Exchange Service (DAAD) to ST. In addition, we would like to thank the scientific reviewers for their detailed and careful reviewing of the manuscript.

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Authors and Affiliations

  1. Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, 79005, Ukraine

    Stepan Tistechok, Victor Fedorenko & Oleksandr Gromyko

  2. Department of Pharmaceutical Biotechnology, Saarland University, 66123, Saarbruecken, Germany

    Maksym Myronovskyi & Andriy Luzhetskyy

  3. Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 66123, Saarbruecken, Germany

    Andriy Luzhetskyy

  4. Microbial Culture Collection of Antibiotic Producers, Ivan Franko National University of Lviv, Lviv, 79005, Ukraine

    Oleksandr Gromyko

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  1. Stepan Tistechok
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  5. Oleksandr Gromyko
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Contributions

OG conceived and planned the experiments. ST and OG performed the screening of the thiopeptides. MM performed the metabolite extraction and identified the berninamycins. ST performed the phylogenetic and multilocus sequence analyses, the genome sequencing, and the identification of the berninamycin gene cluster. ST and OG both processed the experimental data, performed the analysis, drafted the manuscript, and designed the figures. VF and AL aided in interpreting the results and also worked on the manuscript. All the authors read and approved the manuscript.

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Correspondence to Oleksandr Gromyko.

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Tistechok, S., Myronovskyi, M., Fedorenko, V. et al. Screening of Thiopeptide-Producing Streptomycetes Isolated From the Rhizosphere Soil of Juniperus excelsa. Curr Microbiol 79, 305 (2022). https://doi.org/10.1007/s00284-022-03004-2

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