Abstract
The purpose of the study was to which investigate whether dexamethasone, which has anti-inflammatory and immune response suppression roles, could treat noise-induced hearing loss caused by damage to hair cells in the cochlea. The experiment used 8-week-old CBA mice exposed to white noise at an intensity of 110 dB SPL for 2 h, with hearing loss confirmed by the auditory brainstem response test. Dexamethasone was administered by intraperitoneal injection for 5 days, and the therapeutic effect was investigated for 3 weeks. The experimental groups were 3 mg/kg of dexamethasone (3 mpk) and 10 mg/kg of dexamethasone (10 mpk), and the control group was a saline-administered group. The results showed that compared to the control group, the hearing threshold value was recovered by 10 dB SPL compared to the saline group from the 14th day in the 3 mpk group. In the 10 mpk group, thresholds were recovered from the 7th day compared to the saline group. This difference was similar at 4 kHz, and in the case of the 10 mpk group, the threshold was recovered by 20 dB SPL compared to the saline group. The study also confirmed the restoration of nerve cell activity and showed a recovery effect of about 20 µV in the amplitude value change in the 10 mpk group. In conclusion, the study suggests that dexamethasone has a therapeutic effect for noise-induced hearing loss by increasing the activity of nerve cells and showing a recovery effect from hair cells damaged by noise.
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Data availability
The datasets generated in the current study are available from the corresponding author upon reasonable request.
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Acknowledgments
This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIT; 2020R1A2C2003529 to K.Y.L., 2022R1A2C3003700 to U.K.K., and 2022R1F1A1068420 to B.L.). Korea Health industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HR22C1832 to K.Y.L.) and the Bio & Medical Technology Development Program of the NRF funded by MSIT (2022M3E5F2017487 to U.K.K.).
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Conceptualization: K-YL, U-KK, and BL; data curation was analysis: T-JK, BL, M-CK, and Y-RK; experiment analysis: BL, M-CK, Y-RK, and T-JK; data visualization: DJJ, M-CK, J-HK, and BL; writing—review and editing: M-CK and BL. All authors have read and approved the final manuscript.
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All animal procedures were conducted in accordance with the Institutional Animal Care guidelines issued by the Committee of Animal Research of Kyungpook National University (No. 2022-0147). The authors confirm that all methods were carried out in accordance with relevant guidelines and veterinary regulations, and reported in accordance with the ARRIVE guidelines as applicable.
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Communicated by Bill J Yates.
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Lee, B., Kim, MC., Kim, YR. et al. Therapeutic effect of intraperitoneal dexamethasone on noise-induced permanent threshold shift in mice model. Exp Brain Res 242, 257–265 (2024). https://doi.org/10.1007/s00221-023-06742-2
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DOI: https://doi.org/10.1007/s00221-023-06742-2