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Enzyme-targeted near-infrared fluorescent probe for organophosphorus pesticide residue detection

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A Correction to this article was published on 19 December 2023

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Abstract

Pesticide residues significantly affect food safety and harm human health. In this work, a series of near-infrared fluorescent probes were designed and developed by acylating the hydroxyl group of the hemicyanine skeleton with a quenching moiety for monitoring the presence of organophosphorus pesticides in food and live cells. The carboxylic ester bond on the probe was hydrolyzed catalytically in the presence of carboxylesterase and thereby the fluorophore was released with near-infrared emission. Notably, the proposed probe 1 exhibited excellent sensitivity against organophosphorus based on the carboxylesterase inhibition mechanism and the detection limit for isocarbophos achieved 0.1734 μg/L in the fresh vegetable sample. More importantly, probe 1 allowed for situ visualization of organophosphorus in live cells and bacteria, meaning great potential for tracking the organophosphorus in biological systems. Consequently, this study presents a promising strategy for tracking pesticide residues in food and biological systems.

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Funding

This work was financially supported by the Program for Science & Technology Innovation Platform of Hunan Province (2019TP1029) and Food Safety Monitoring and Early Warning open Project of Hunan Provincial Key Laboratory (2020KFJJ01).

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Authors and Affiliations

  1. Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, Hunan Key Laboratory of Forestry Edible Resources Safety and Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, People’s Republic of China

    Shan Luo, Ruichen Peng, Ying Wang, Jiali Ren, Wang Li, Ying Xiong & Qian Wen

  2. College of Chemistry and Materials Engineering, Huaihua University, Huaihua, 418000, People’s Republic of China

    Sili Yi

  3. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People’s Republic of China

    Xianjun Liu

  4. Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Hunan Institute Food Quality Supervision Inspection and Research, Changsha, 410004, People’s Republic of China

    Qian Wen

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  1. Shan Luo
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  2. Ruichen Peng
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Correspondence to Sili Yi or Qian Wen.

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Luo, S., Peng, R., Wang, Y. et al. Enzyme-targeted near-infrared fluorescent probe for organophosphorus pesticide residue detection. Anal Bioanal Chem 415, 4849–4859 (2023). https://doi.org/10.1007/s00216-023-04801-9

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  • DOI: https://doi.org/10.1007/s00216-023-04801-9

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