Skip to main content
SpringerLink
Log in

A label-free T4 polynucleotide kinase fluorescence sensor based on split dimeric G-quadruplex and ligation-induced dimeric G-quadruplex/thioflavin T conformation

  • Research Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

The phosphorylation process of DNA by T4 polynucleotide kinase (T4 PNK) plays a crucial role in DNA recombination, DNA replication, and DNA repair. Traditional monomeric G-quadruplex (G4) systems are always activated by single cation such as K+ or Na+. The conformation transformation caused by the coexistence of multiple cations may interfere with the signal readout and limit their applications in physiological system. In view of the stability of dimeric G4 in multiple cation solution, we reported a label-free T4 PNK fluorescence sensor based on split dimeric G4 and ligation-induced dimeric G4/thioflavin T (ThT) conformation. The dimeric G4 was divided into two independent pieces of one normal monomeric G4 and the other monomeric G4 fragment phosphorylated by T4 PNK in order to decrease the background signal. With the introduction of template DNA, DNA ligase, and invasive DNA, the dimeric G4 could be generated and liberated to combine with ThT to show obvious fluorescence signal. Using our strategy, the linear range from 0.005 to 0.5 U mL−1, and the detection limit of 0.0021 U mL−1 could be achieved without the consideration of interference caused by the coexistence of multiple cations. Additionally, research in real sample determination and inhibition effect investigations indicated its further potential application value in biochemical process research and clinic diagnostics.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Richardson CC. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965;54(1):158–65. https://doi.org/10.1073/pnas.54.1.158.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Novogrodsky A, Hurwitz J. The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5’-hydroxyl termini. J Biol Chem. 1966;241(12):2923–32. https://doi.org/10.1016/S0021-9258(18)96553-1.

    Article  CAS  PubMed  Google Scholar 

  3. Ma C, Yeung ES. Highly sensitive detection of DNA phosphorylation by counting single nanoparticles. Anal Bioanal Chem. 2010;397(6):2279–84. https://doi.org/10.1007/s00216-010-3801-x.

    Article  CAS  PubMed  Google Scholar 

  4. Karimi-Busheri F, Daly G, Robins P, Canas B, Pappin DJ, Sgouros J, Miller GG, Fakhrai H, Davis EM, Le Beau MM, Weinfeld M. Molecular characterization of a human DNA kinase. J Biol Chem. 1999;274(34):24187–94. https://doi.org/10.1074/jbc.274.34.24187.

    Article  CAS  PubMed  Google Scholar 

  5. Chalasani SL, Kawale AS, Akopiants K, Yu Y, Fanta M, Weinfeld M, Povirk LF. Persistent 3’-phosphate termini and increased cytotoxicity of radiomimetic DNA double-strand breaks in cells lacking polynucleotide kinase/phosphatase despite presence of an alternative 3’-phosphatase. DNA Repair (Amst). 2018;68:12–24. https://doi.org/10.1016/j.dnarep.2018.05.002.

    Article  CAS  Google Scholar 

  6. Tang Z, Wang K, Tan W, Ma C, Li J, Liu L, Guo Q, Meng X. Real-time investigation of nucleic acids phosphorylation process using molecular beacons. Nucleic Acids Res. 2005;33(11): e97. https://doi.org/10.1093/nar/gni096.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Sharma S, Doherty KM, Brosh RJ. Mechanisms of RecQ helicases in pathways of DNA metabolism and maintenance of genomic stability. Biochem J. 2006;398(3):319–37. https://doi.org/10.1042/BJ20060450.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Tahbaz N, Subedi S, Weinfeld M. Role of polynucleotide kinase/phosphatase in mitochondrial DNA repair. Nucleic Acids Res. 2012;40(8):3484–95. https://doi.org/10.1093/nar/gkr1245.

    Article  CAS  PubMed  Google Scholar 

  9. Ahel I, Rass U, El-Khamisy SF, Katyal S, Clements PM, Mckinnon PJ, Caldecott KW, West SC. The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates. Nature. 2006;443(7112):713–6. https://doi.org/10.1038/nature05164.

    Article  CAS  PubMed  Google Scholar 

  10. Aggarwal M, Banerjee T, Sommers JA, Iannascoli C, Pichierri P, Shoemaker RH, Brosh RM. Werner syndrome helicase has a critical role in DNA damage responses in the absence of a functional Fanconi anemia pathway. Cancer Res. 2013;73(17):5497–507. https://doi.org/10.1158/0008-5472.CAN-12-2975.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Amitsur M, Levitz R, Kaufmann G. Bacteriophage T4 anticodon nuclease, polynucleotide kinase and RNA ligase reprocess the host lysine tRNA. Embo J. 1987;6(8):2499–503. https://doi.org/10.1002/j.1460-2075.1987.tb02532.x.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Chappell C, Hanakahi LA, Karimi-Busheri F, Weinfeld M, West SC. Involvement of human polynucleotide kinase in double-strand break repair by non-homologous end joining. Embo J. 2002;21(11):2827–32. https://doi.org/10.1093/emboj/21.11.2827.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Phillips DH, Arlt VM. The 32P-postlabeling assay for DNA adducts. Nat Protoc. 2007;2(11):2772–81. https://doi.org/10.1038/nprot.2007.394.

    Article  CAS  PubMed  Google Scholar 

  14. Wang LK, Shuman S. Domain structure and mutational analysis of T4 polynucleotide kinase. J Biol Chem. 2001;276(29):26868–74. https://doi.org/10.1074/jbc.M103663200.

    Article  CAS  PubMed  Google Scholar 

  15. Whitehouse CJ, Taylor RM, Thistlethwaite A, Zhang H, Karimi-Busheri F, Lasko DD, Weinfeld M, Caldecott KW. XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair. Cell. 2001;104(1):107–17. https://doi.org/10.1016/s0092-8674(01)00195-7.

    Article  CAS  PubMed  Google Scholar 

  16. Cen Y, Deng WJ, Yu RQ, Chu X. Sensitive fluorescence sensing of T4 polynucleotide kinase activity and inhibition based on DNA/polydopamine nanospheres platform. Talanta. 2018;180:271–6. https://doi.org/10.1016/j.talanta.2017.12.038.

    Article  CAS  PubMed  Google Scholar 

  17. Chen F, Zhao Y, Qi L, Fan C. One-step highly sensitive florescence detection of T4 polynucleotide kinase activity and biological small molecules by ligation-nicking coupled reaction-mediated signal amplification. Biosen Bioelectron. 2013;47:218–24. https://doi.org/10.1016/j.bios.2013.03.034.

    Article  CAS  Google Scholar 

  18. Gao M, Guo J, Song Y, Zhu Z, Yang CJ. Detection of T4 polynucleotide kinase via allosteric aptamer probe platform. ACS Appl Mater Interfaces. 2017;9(44):38356–63. https://doi.org/10.1021/acsami.7b14185.

    Article  CAS  PubMed  Google Scholar 

  19. Hou T, Wang X, Liu X, Lu T, Liu S, Li F. Amplified detection of T4 polynucleotide kinase activity by the coupled lambda exonuclease cleavage reaction and catalytic assembly of bimolecular beacons. Anal Chem. 2014;86(1):884–90. https://doi.org/10.1021/ac403458b.

    Article  CAS  PubMed  Google Scholar 

  20. Liu S, Ming J, Lin Y, Wang C, Liu T, Cheng C, Li F. Amplified detection of T4 polynucleotide kinase activity based on a λ-exonuclease cleavage-induced DNAzyme releasing strategy. Sensors Actuators B Chem. 2014;192:157–63. https://doi.org/10.1016/j.snb.2013.10.101.

    Article  CAS  Google Scholar 

  21. Zhang X, Zheng C, Ding L, Wu Y, Xu H, Sun Y, Zeng Y, Liu X, Liu J. CRISPR-Cas12a coupled with terminal deoxynucleotidyl transferase mediated isothermal amplification for sensitive detection of polynucleotide kinase activity. Sensors Actuators B Chem. 2021;330: 129317. https://doi.org/10.1016/j.snb.2020.129317.

    Article  CAS  Google Scholar 

  22. Zhou H, Tong C, Zou W, Yang Y, Liu Y, Li B, Qin Y, Dang W, Liu B, Wang W. A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction. Analyst. 2019;144(4):1187–96. https://doi.org/10.1039/c8an02147c.

    Article  CAS  PubMed  Google Scholar 

  23. Zhu Z, Yu R, Chu X. Amplified fluorescence detection of T4 polynucleotide kinase activity and inhibition via a coupled λ exonuclease reaction and exonuclease III-aided trigger DNA recycling. Anal Methods. 2014;6(15):6009–14. https://doi.org/10.1039/C4AY01097C.

    Article  CAS  Google Scholar 

  24. Shen X, Ge J, Chen J, Shen Y, Meng H, Li Z, Qu L. A novel fluorescence method for the highly sensitive detection of T4 polynucleotide kinase based on polydopamine nanotubes. New J Chem. 2019;43:16753–8. https://doi.org/10.1039/C9NJ04381K.

    Article  CAS  Google Scholar 

  25. Luo R, Zhou H, Dang W, Long Y, Tong C, Xie Q, Daniyal M, Liu B, Wang W. A DNAzyme-rGO coupled fluorescence assay for T4PNK activity in vitro and intracellular imaging. Sensors Actuators B Chem. 2020;310: 127884. https://doi.org/10.1016/j.snb.2020.127884.

    Article  CAS  Google Scholar 

  26. Chen X, Cao G, Zhang J, Deng Y, Luo X, Yang M, Huo D, Hou C. An ultrasensitive and point-of-care strategy for enzymes activity detection based on enzyme extends activators to unlock the ssDNase activity of CRISPR/Cas12a (EdU-CRISPR/Cas12a). Sensors Actuators B Chem. 2021;333: 129553. https://doi.org/10.1016/j.snb.2021.129553.

    Article  CAS  Google Scholar 

  27. Liu J, Liu Y, Zhang L, Fu S, Su X. Ultra-specific fluorescence detection of DNA modifying enzymes by dissipation system. Biosens Bioelectron. 2022;215: 114561. https://doi.org/10.1016/j.bios.2022.114561.

    Article  CAS  PubMed  Google Scholar 

  28. Shang J, Yu S, Chen Y, Gao Y, Hong C, Li F, Wang F. Real-time investigation of intracellular polynucleotide kinase using a cascaded amplification circuit. Anal Chem. 2021;93(46):15559–66. https://doi.org/10.1021/acs.analchem.1c04033.

    Article  CAS  PubMed  Google Scholar 

  29. Jiang HX, Kong DM, Shen HX. Amplified detection of DNA ligase and polynucleotide kinase/phosphatase on the basis of enrichment of catalytic G-quadruplex DNAzyme by rolling circle amplification. Biosens Bioelectron. 2014;55:133–8. https://doi.org/10.1016/j.bios.2013.12.001.

    Article  CAS  PubMed  Google Scholar 

  30. Liu H, Ma C, Wang J, Chen H, Wang K. Label-free colorimetric assay for T4 polynucleotide kinase/phosphatase activity and its inhibitors based on G-quadruplex/hemin DNAzyme. Anal Biochem. 2017;517:18–21. https://doi.org/10.1016/j.ab.2016.10.022.

    Article  CAS  PubMed  Google Scholar 

  31. Jiang C, Yan C, Jiang J, Yu R. Colorimetric assay for T4 polynucleotide kinase activity based on the horseradish peroxidase-mimicking DNAzyme combined with λ exonuclease cleavage. Anal Chim Acta. 2013;766:88–93. https://doi.org/10.1016/j.aca.2012.12.034.

    Article  CAS  PubMed  Google Scholar 

  32. Jiang Y, Cui J, Zhang T, Wang M, Zhu G, Miao P. Electrochemical detection of T4 polynucleotide kinase based on target-assisted ligation reaction coupled with silver nanoparticles. Anal Chim Acta. 2019;1085:85–90. https://doi.org/10.1016/j.aca.2019.07.072.

    Article  CAS  PubMed  Google Scholar 

  33. Zhang G, Chai H, Tian M, Zhu S, Qu L, Zhang X. Zirconium-metalloporphyrin frameworks-luminol competitive electrochemiluminescence for ratiometric detection of polynucleotide kinase activity. Anal Chem. 2020;92(10):7354–62. https://doi.org/10.1021/acs.analchem.0c01262.

    Article  CAS  PubMed  Google Scholar 

  34. Song Z, Li Y, Teng H, Ding C, Xu G, Luo X. Designed zwitterionic peptide combined with sacrificial Fe-MOF for low fouling and highly sensitive electrochemical detection of T4 polynucleotide kinase. Sensors Actuators B Chem. 2020;305: 127329. https://doi.org/10.1016/j.snb.2019.127329.

    Article  CAS  Google Scholar 

  35. Zhang Q, Li Z, Zhou Y, Li X, Li B, Yin H, Ai S. Electrochemical biosensors for polynucleotide kinase activity assay and inhibition screening based on phosphorylation reaction triggered λ exonuclease and exonuclease I cleavage. Sensors Actuators B Chem. 2016;225:151–7. https://doi.org/10.1016/j.snb.2015.11.033.

    Article  CAS  Google Scholar 

  36. Hou T, Wang X, Liu X, Pan C, Li F. Sensitive electrochemical assay for T4 polynucleotide kinase activity based on dual-signaling amplification coupled with exonuclease reaction. Sensors Actuators B Chem. 2014;202:588–93. https://doi.org/10.1016/j.snb.2014.06.003.

    Article  CAS  Google Scholar 

  37. Zhang Y, Fang X, Zhu Z, Lai Y, Xu C, Pang P, Wang H, Yang C, Barrow CJ, Yang W. A sensitive electrochemical assay for T4 polynucleotide kinase activity based on titanium dioxide nanotubes and a rolling circle amplification strategy. RSC Adv. 2018;8(67):38436–44. https://doi.org/10.1039/C8RA07745B.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Mao J, Chen X, Xu H, Xu X. DNAzyme-driven DNA walker biosensor for amplified electrochemical detection of T4 polynucleotide kinase activity and inhibition. J Electroanal Chem. 2020;874: 114470. https://doi.org/10.1016/j.jelechem.2020.114470.

    Article  CAS  Google Scholar 

  39. Du J, Xu Q, Lu X, Zhang CY. A label-free bioluminescent sensor for real-time monitoring polynucleotide kinase activity. Anal Chem. 2014;86(16):8481–8. https://doi.org/10.1021/ac502240c.

    Article  CAS  PubMed  Google Scholar 

  40. Li P, Cao Y, Mao C, Jin B, Zhu J. TiO2/g-C3N4/CdS nanocomposite-based photoelectrochemical biosensor for ultrasensitive evaluation of T4 polynucleotide kinase activity. Anal Chem. 2019;91(2):1563–70. https://doi.org/10.1021/acs.analchem.8b04823.

    Article  CAS  PubMed  Google Scholar 

  41. Yan Z, Shen X, Zhou B, Pan R, Zhang B, Zhao C, Ren L, Ming J. Precise analysis of T4 polynucleotide kinase and inhibition by coupling personal glucose meter with split DNAzyme and ligation-triggered DNA walker. Sensors Actuators B Chem. 2021;326: 128831. https://doi.org/10.1016/j.snb.2020.128831.

    Article  CAS  Google Scholar 

  42. Jiang H, Xu Y, Dai L, Liu X, Kong D. Ultrasensitive, label-free detection of T4 ligase and T4 polynucleotide kinase based on target-triggered hyper-branched rolling circle amplification. Sensors Actuators B Chem. 2018;260:70–7. https://doi.org/10.1016/j.snb.2017.12.203.

    Article  CAS  Google Scholar 

  43. Wu X, He S, Zhao JX. Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity. Anal Methods. 2020;12(6):807–12. https://doi.org/10.1039/C9AY02283J.

    Article  CAS  Google Scholar 

  44. Chen H, Wang Z, Chen X, Lou K, Sheng A, Chen T, Chen G, Zhang J. New method for detection of T4 polynucleotide kinase phosphatase activity through isothermal EXPonential amplification reaction. Analyst. 2019;144(6):1955–9. https://doi.org/10.1039/c8an02368a.

    Article  CAS  PubMed  Google Scholar 

  45. Li X, Xu X, Song J, Xue Q, Li C, Jiang W. Sensitive detection of T4 polynucleotide kinase activity based on multifunctional magnetic probes and polymerization nicking reactions mediated hyperbranched rolling circle amplification. Biosens Bioelectron. 2017;91:631–6. https://doi.org/10.1016/j.bios.2017.01.022.

    Article  CAS  PubMed  Google Scholar 

  46. Wang M, Kong D, Su D, Liu Y, Su X. Ratio fluorescence analysis of T4 polynucleotide kinase activity based on the formation of a graphene quantum dot-copper nanocluster nanohybrid. Nanoscale. 2019;11(29):13903–8. https://doi.org/10.1039/c9nr02901j.

    Article  CAS  PubMed  Google Scholar 

  47. Wang M, Chen J, Jiang S, Nie Y, Su X. Rapid synthesis of dual proteins co-functionalized gold nanoclusters for ratiometric fluorescence sensing of polynucleotide kinase activity. Sensors Actuators B Chem. 2021;329: 129200. https://doi.org/10.1016/j.snb.2020.129200.

    Article  CAS  Google Scholar 

  48. Li J, Ma J, Zhang Y, Zhang Z, He G. A fluorometric method for determination of the activity of T4 polynucleotide kinase by using a DNA-templated silver nanocluster probe. Mikrochim Acta. 2019;186(1):48–54. https://doi.org/10.1007/s00604-018-3157-z.

    Article  CAS  PubMed  Google Scholar 

  49. Zhao H, Yan Y, Chen M, Hu T, Wu K, Liu H, Ma C. Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles. Analyst. 2019;144(22):6689–97. https://doi.org/10.1039/c9an01659g.

    Article  CAS  PubMed  Google Scholar 

  50. Zhu J, Chen L. Highly efficient incorporation of dATP in terminal transferase polymerization forming the ploy (A)n-DITO-1 fluorescent probe sensing terminal transferase and T4 polynucleotide kinase activity. Anal Chim Acta. 2022;1221: 340080. https://doi.org/10.1016/j.aca.2022.340080.

    Article  CAS  PubMed  Google Scholar 

  51. Huang Z, Zou J, Li X, He Q, Nie J. Research progress of fluorescent probe for G-quadruplex. Chin J Anal Chem 2021;49(8):1258–1269. https://doi.org/10.19756/j.issn.0253-3820.201797.

  52. Zhou F, Wang G, Shi D, Sun Y, Sha L, Qiu Y, Zhang X. One-strand oligonucleotide probe for fluorescent label-free “turn-on” detection of T4 polynucleotide kinase activity and its inhibition. Analyst. 2015;140(16):5650–5. https://doi.org/10.1039/c5an00862j.

    Article  CAS  PubMed  Google Scholar 

  53. Mohanty J, Barooah N, Dhamodharan V, Harikrishna S, Pradeepkumar PI, Bhasikuttan AC. Thioflavin T as an efficient inducer and selective fluorescent sensor for the human telomeric G-quadruplex DNA. J Am Chem Soc. 2013;135(1):367–76. https://doi.org/10.1021/ja309588h.

    Article  CAS  PubMed  Google Scholar 

  54. Huang C, Shen G, Ding S, Kan A, Jiang D, Jiang W. Primer-template conversion-based cascade signal amplification strategy for sensitive and accurate detection of polynucleotide kinase activity. Anal Chim Acta. 2021;1187: 339139. https://doi.org/10.1016/j.aca.2021.339139.

    Article  CAS  PubMed  Google Scholar 

  55. Zhao H, Liu Q, Liu M, Jin Y, Li B. Label-free fluorescent assay of T4 polynucleotide kinase phosphatase activity based on G-quadruplex-thioflavin T complex. Talanta. 2017;165:653–8. https://doi.org/10.1016/j.talanta.2017.01.027.

    Article  CAS  PubMed  Google Scholar 

  56. Wang C, Li J. Fluorescence method for kanamycin detection based on the conversion of G-triplex and G-quadruplex. Anal Bioanal Chem. 2021;413(28):7073–80. https://doi.org/10.1007/s00216-021-03676-y.

    Article  CAS  PubMed  Google Scholar 

  57. Shi Z, Zhang X, Cheng R, Li B, Jin Y. A label-free cyclic assembly of G-quadruplex nanowires for cascade amplification detection of T4 polynucleotide kinase activity and inhibition. Analyst. 2015;140(17):6124–30. https://doi.org/10.1039/c5an00968e.

    Article  CAS  PubMed  Google Scholar 

  58. Cheng R, Tao M, Shi Z, Zhang X, Jin Y, Li B. Label-free and sensitive detection of T4 polynucleotide kinase activity via coupling DNA strand displacement reaction with enzymatic-aided amplification. Biosen Bioelectron. 2015;73:138–45. https://doi.org/10.1016/j.bios.2015.05.059.

    Article  CAS  Google Scholar 

  59. Guo Y, Wang Q, Wang Z, Chen X, Xu L, Hu J, Pei R. Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes. Sensors Actuators B Chem. 2015;214:50–5. https://doi.org/10.1016/j.snb.2015.03.013.

    Article  CAS  Google Scholar 

  60. Deng S, Zhou B, Li W, Li H, Zhang F, Ming J. Label-free fluorescence DNA walker for protein analysis based on terminal protection and dual enzyme assisted cleavage induced G-quadruplex/berberine conformation. Analyst. 2020;145(1):46–51. https://doi.org/10.1039/C9AN01853K.

    Article  CAS  Google Scholar 

  61. Cheng Y, Cheng M, Hao J, Miao W, Zhou W, Jia G, Li C. Highly selective detection of K+ based on a dimerized G-quadruplex DNAzyme. Anal Chem. 2021;93(18):6907–12. https://doi.org/10.1021/acs.analchem.1c00872.

    Article  CAS  PubMed  Google Scholar 

  62. Ma G, Yu Z, Zhou W, Li Y, Fan L, Li X. Investigation of Na+ and K+ competitively binding with a G-quadruplex and discovery of a stable K+-Na+-quadruplex. J Phys Chem B. 2019;123(26):5405–11. https://doi.org/10.1021/acs.jpcb.9b02823.

    Article  CAS  PubMed  Google Scholar 

  63. Wang Z, Li M, Hsu SD, Chang T. Structural basis of sodium-potassium exchange of a human telomeric DNA quadruplex without topological conversion. Nucleic Acids Res. 2014;42(7):4723–33. https://doi.org/10.1093/nar/gku083.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  64. Sun H, Xiang J, Gai W, Liu Y, Guan A, Yang Q, Li Q, Shang Q, Su H, Tang Y, Xu G. Quantification of the Na+/K+ ratio based on the different response of a newly identified G-quadruplex to Na+ and K+. Chem Commun. 2013;49(40):4510–2. https://doi.org/10.1039/c3cc39020a.

    Article  CAS  Google Scholar 

  65. Jing S, Liu Q, Jin Y, Li B. Dimeric G-quadruplex: an effective nucleic acid scaffold for lighting up thioflavin T. Anal Chem. 2021;93(3):1333–41. https://doi.org/10.1021/acs.analchem.0c02637.

    Article  CAS  PubMed  Google Scholar 

  66. Song X, Ding Q, Pu Y, Zhang J, Sun R, Yin L, Wei W, Liu S. Application of the dimeric G-quadruplex and toehold-mediated strand displacement reaction for fluorescence biosensing of ochratoxin A. Biosens Bioelectron. 2021;192: 113537. https://doi.org/10.1016/j.bios.2021.113537.

    Article  CAS  PubMed  Google Scholar 

  67. Ma X, Lv M, Du F, Wu C, Lou B, Zeid AM, Xu G. Dimeric G-quadruplex: an efficient probe for ultrasensitive fluorescence detection of mustard compounds. Anal Chem. 2022;94(9):4112–8. https://doi.org/10.1021/acs.analchem.2c00124.

    Article  CAS  PubMed  Google Scholar 

  68. Jin T, Zhang J, Zhao Y, Huang X, Tan C, Sun S, Tan Y. Magnetic bead-gold nanoparticle hybrids probe based on optically countable gold nanoparticles with dark-field microscope for T4 polynucleotide kinase activity assay. Biosens Bioelectron. 2020;150:11936. https://doi.org/10.1016/j.bios.2019.11936.

    Article  Google Scholar 

  69. Cui L, Li Y, Lu M, Tang B, Zhang C. An ultrasensitive electrochemical biosensor for polynucleotide kinase assay based on gold nanoparticle-mediated lambda exonuclease cleavage-induced signal amplification. Biosens Bioelectron. 2018;99:1–7. https://doi.org/10.1016/j.bios.2017.07.028.

    Article  CAS  PubMed  Google Scholar 

  70. Lin M, Wan H, Zhang J, Wang Q, Hu X, Xia F. Electrochemical DNA sensors based on MoS2-AuNPs for polynucleotide kinase activity and inhibition assay. ACS Appl Mater Interfaces. 2020;12(41):45814–21. https://doi.org/10.1021/acsami.0c13385.

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This work was supported by the PhD Foundation of Weifang Medical University and the Public Domestic Visiting Program of Weifang Medical University (20217–13).

Author information

Authors and Affiliations

  1. School of Pharmacy, Weifang Medical University, Weifang, 261053, People’s Republic of China

    Liuya Wei, Xianglong Kong, Mengran Wang, Ruiyan Pan, Yuanzheng Cheng, Jin Zhou & Jingjing Ming

  2. School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Zhihua Lv

  3. School of Clinical Medicine, Weifang Medical University, Weifang, 261053, People’s Republic of China

    Yixin Zhang

Authors
  1. Liuya Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xianglong Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mengran Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yixin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ruiyan Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuanzheng Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhihua Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jin Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jingjing Ming
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Zhihua Lv, Jin Zhou or Jingjing Ming.

Ethics declarations

Ethics approval

This work has been approved by the Ethics Committee of Weifang Medical University and has been performed in accordance with the ethical standards.

Source of biological material

Human serum samples were obtained from the Affiliated Hospital of Weifang Medical University (Weifang, China). All serum-providing participants provided written informed consent for this study protocol.

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 2.82 MB)

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wei, L., Kong, X., Wang, M. et al. A label-free T4 polynucleotide kinase fluorescence sensor based on split dimeric G-quadruplex and ligation-induced dimeric G-quadruplex/thioflavin T conformation. Anal Bioanal Chem 414, 7923–7933 (2022). https://doi.org/10.1007/s00216-022-04327-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-022-04327-6

Keywords

Search

Navigation