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Near-infrared fluorogenic receptor for selective detection of cysteine in blood serum and living cells

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Abstract

A novel near-infrared fluorescent probe, namely propane-2,2-diylbis(2-((E)-2-(benzo[d]thiazol-2-yl)-2-cyanovinyl)-4,1-phenylene) diacrylate (BTA), was synthesized for selective detection of cysteine over other biologically significant amino acids. Upon addition of cysteine, the probe BTA displays a dramatic increase in fluorescence intensity at 715 nm along with a fast response time (4 min). The limit of detection (LOD) was calculated as 0.12 μM. In addition, the synthesized probe BTA was effectively utilized for the recognition of cysteine in blood serum and living cells.

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Acknowledgments

One of the authors (A.G) thanks the Department of Science and Technology, Ministry of Science and Technology, Government of India, for a fellowship under the INSPIRE scheme (No. IF140098). The authors also express their sincere thanks to the Council of Scientific and Industrial Research (CSIR), New Delhi, India [Grant No. 01 (2907)/17/EMR-II] and University Grants Commission (UGC), India [SAP grant No. 540/20/DRS-I/2016(SAP-I)] for financial assistance.

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Authors and Affiliations

  1. Department of Chemistry, Periyar University, Salem, Tamil Nadu, 636011, India

    Gomathi Asaithambi & Viswanathamurthi Periasamy

  2. Department of Chemistry, National Institute of Technology, Tiruchirappalli, Tamil Nadu, 620015, India

    Haribabu Jebiti

Authors
  1. Gomathi Asaithambi
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  2. Viswanathamurthi Periasamy
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  3. Haribabu Jebiti
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Contributions

Conceptualization: Periasamy Viswanathamurthi; methodology: Asaithambi Gomathi; formal analysis and investigation: Asaithambi Gomathi and Jebiti Haribabu; writing—original draft preparation: Asaithambi Gomathi; writing—review and editing: Periasamy Viswanathamurthi; funding acquisition: Periasamy Viswanathamurthi; resources: Periasamy Viswanathamurthi; supervision: Periasamy Viswanathamurthi.

Corresponding author

Correspondence to Viswanathamurthi Periasamy.

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The biological studies have been approved by the Human Ethics Committee (Government Mohan Kumaramangalam Medical College and Hospital/4341/IEC/2019-306) and have been performed in accordance with the ethical standards.

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The authors declare that they have no conflict of interest.

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Asaithambi, G., Periasamy, V. & Jebiti, H. Near-infrared fluorogenic receptor for selective detection of cysteine in blood serum and living cells. Anal Bioanal Chem 413, 1817–1826 (2021). https://doi.org/10.1007/s00216-020-03149-8

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  • DOI: https://doi.org/10.1007/s00216-020-03149-8

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